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[189050_at] DOM34 protein

Description R74.6 /REP_DB=WormBase Gene ID /WP=CE01059 /TR=SW:P50444 /GB=CAA85277.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=DOM34 protein
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 09      4grp_highest_8_8 > 43      4grp_high_8_8 > 48      6grp_highest_nonorm > 71      6grp_total > 03      4grp_total > 55      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 298
1 2 3 4 range
A 533.7
(3)
P 		235.7
(-3)
P 		352.6
(-0.6)
P 		358
(-0.5)
P 		298
B 425
(3)
P 		312.1
(-2.2)
P 		328.1
(-1.4)
P 		293.9
(-3)
P 		131
C 442
(3)
P 		401.3
(-0.7)
P 		433
(2.2)
P 		376.7
(-3)
P 		65
avr 466.9
(3) 		316.4
(-3) 		371.2
(-0.8) 		342.9
(-1.9) 		151

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 209
1 2 3 4 5 6 range
A 388
(1.8)
P 		349.3
(-0.1)
P 		289.4
(-3)
P 		394.7
(2.1)
P 		412.5
(3)
P 		296.1
(-2.7)
P 		123
B 461.2
(3)
P 		433.2
(2.2)
P 		252.6
(-3)
P 		328.8
(-0.8)
P 		365.7
(0.3)
P 		347.8
(-0.3)
P 		209
C 423.4
(2.8)
P 		427.6
(3)
P 		276.5
(-3)
P 		399.8
(1.9)
P 		336.4
(-0.6)
P 		281.4
(-2.8)
P 		151
avr 424.2
(3) 		403.4
(2.2) 		272.8
(-3) 		374.4
(1) 		371.5
(0.9) 		308.4
(-1.6) 		151
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 143
1 2 3 4 range
A 194.3
(3)
P 		51.2
(-3)
P 		92.2
(-1.3)
P 		52.4
(-2.9)
P 		143
B 135.5
(3)
P 		77.1
(-1.8)
P 		76
(-1.9)
P 		62.6
(-3)
P 		73
C 146.3
(2.7)
P 		115.4
(0.6)
P 		150.9
(3)
P 		63.5
(-3)
P 		87
avr 158.7
(3) 		81.2
(-1.7) 		106.4
(-0.2) 		59.5
(-3) 		99

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 71.5
(-2.2)
P 		124.2
(3)
P 		63
(-3)
P 		100.1
(0.6)
P 		124.5
(3)
P 		72.1
(-2.1)
P 		62
B 160.5
(3)
P 		144.2
(2)
P 		61.3
(-3)
P 		87.1
(-1.4)
P 		148.6
(2.3)
P 		129.7
(1.1)
P 		99
C 157
(3)
P 		130.5
(1.2)
P 		70.4
(-3)
P 		80.4
(-2.3)
P 		108.8
(-0.3)
P 		94.2
(-1.4)
P 		87
avr 129.7
(2.7) 		133
(3) 		64.9
(-3) 		89.2
(-0.9) 		127.3
(2.5) 		98.7
(-0) 		68
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189050_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7237
Sequence Type Exemplar sequence
Representative Public ID R74.6 NCBI
Target Description R74.6 /REP_DB=WormBase Gene ID /WP=CE01059 /TR=SW:P50444 /GB=CAA85277.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=DOM34 protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065441(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065441 NCBI Caenorhabditis elegans R74.6 (R74.6) mRNA, complete cds. 11/11 None
SNAP00000044751 ENSEMBL cdna:SNAP chromosome:CEL140:III:4202345:4206193:-1 11/11 None
GENEFINDER00000044755 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4202345:4204158:-1 11/11 None
R74.6 ENSEMBL cdna:known chromosome:CEL140:III:4202345:4204158:-1 gene:R74.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4202344-4204158(-) 100.0 100.0
Public Domain and Genome References
Gene Title DOM34 protein
Ensembl R74.6
Entrez Gene 187888 Entrez gene
SwissProt P50444 EMBL-EBI
WormBase CE01059 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:16551_S_AT pelota (PEL1) at
ATH1-121501:253882_AT pelota (PEL1) at
CANINE_2:CFAAFFX.28214.1.S1_AT similar to Pelota homolog cfa
DROSOPHILA_2:1631161_AT pelota dm
DROSGENOME1:154677_AT pelota dm
CHICKEN:GGAAFFX.12386.1.S1_AT pelota homolog (Drosophila) gga
CHICKEN:GGAAFFX.8764.1.S1_S_AT pelota homolog (Drosophila) gga
HG-U133A:218472_S_AT pelota homolog (Drosophila) hs
HG-U133_PLUS_2:218472_S_AT pelota homolog (Drosophila) hs
HG-U133A_2:218472_S_AT pelota homolog (Drosophila) hs
U133_X3P:G7705599_3P_S_AT pelota homolog (Drosophila) hs
U133_X3P:HS2.196972.1.A1_3P_AT pelota homolog (Drosophila) hs
HG-U133_PLUS_2:1553140_AT pelota homolog (Drosophila) hs
HG-U95C:57628_AT pelota homolog (Drosophila) hs
HG-U95E:69325_AT pelota homolog (Drosophila) hs
HU35KSUBA:RC_AA156873_AT Pelota homolog (Drosophila) hs
HU35KSUBB:RC_AA151210_AT Pelota homolog (Drosophila) hs
HU35KSUBD:RC_AA450324_AT Pelota homolog (Drosophila) hs
U133_X3P:HS2.420404.1.S1_3P_AT Pelota homolog (Drosophila) hs
U133_X3P:HS.184411.5.S1_3P_AT Pelota homolog (Drosophila) hs
U133_X3P:HS.116774.0.S1_3P_AT Pelota homolog (Drosophila) hs
HG-U133_PLUS_2:1560359_AT Pelota homolog (Drosophila) hs
HG-U133A_2:214660_AT Pelota homolog (Drosophila) hs
HG-U133_PLUS_2:214660_AT Pelota homolog (Drosophila) hs
HG-U133A:214660_AT Pelota homolog (Drosophila) hs
HG-U133_PLUS_2:226731_AT Pelota homolog (Drosophila) hs
HG-U133B:226731_AT Pelota homolog (Drosophila) hs
HG-U95B:44710_AT Pelota homolog (Drosophila) hs
HG-U95B:45200_AT Pelota homolog (Drosophila) hs
HG-U95C:61847_AT Pelota homolog (Drosophila) hs
HG-U95D:89701_AT Pelota homolog (Drosophila) hs
HU35KSUBA:AA436974_AT Pelota homolog (Drosophila) hs
HU35KSUBB:RC_Z39294_I_AT Pelota homolog (Drosophila) hs
HU35KSUBD:RC_AA620814_AT Pelota homolog (Drosophila) hs
MU11KSUBA:AA409897_RC_S_AT pelota homolog (Drosophila) mm
MG-U74AV2:98017_AT pelota homolog (Drosophila) mm
MG-U74AV2:161280_R_AT pelota homolog (Drosophila) mm
MOE430A:1434071_A_AT pelota homolog (Drosophila) mm
MOUSE430A_2:1434071_A_AT pelota homolog (Drosophila) mm
MOUSE430_2:1434071_A_AT pelota homolog (Drosophila) mm
MU19KSUBB:TC31389_AT Pelota homolog (Drosophila) (Pelo), mRNA mm
RG-U34A:RC_AA799330_AT pelota homolog rn
RAE230A:1371684_AT pelota homolog rn
RAT230_2:1371684_AT pelota homolog rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA85277 Hypothetical protein R74.6 [Caenorhabditis elegans] ref|NP_497842.1| R74.6 [Caenorhabditis elegans] sp|P50444|YNU6_CAEEL Hypothetical protein R74.6 in chromosome III 0.0
blast CAE72864 Hypothetical protein CBG20163 [Caenorhabditis briggsae] 0.0
blast CAE72864 Hypothetical protein CBG20163 [Caenorhabditis briggsae] 1.0E-177
blast EAA12313 ENSANGP00000006742 [Anopheles gambiae str. PEST] ref|XP_317201.2| ENSANGP00000006742 [Anopheles gambiae str. PEST] 1.0E-116
Protein Domains
Database ID Description E-Value
Pfam IPR005141 EMBL-EBI eRF1 domain 2 7.3E-50
Pfam IPR005142 EMBL-EBI eRF1 domain 3 2.6E-42
Pfam IPR005140 EMBL-EBI eRF1 domain 1 1.9E-48
Pfam IPR005141 EMBL-EBI eRF1 domain 2 7.3E-50
Pfam IPR005142 EMBL-EBI eRF1 domain 3 2.6E-42
Pfam IPR005140 EMBL-EBI eRF1 domain 1 1.4E-43
Sequence
Target Sequence 
>C. ELEGANS:189050_AT
gtcagtacggctttcatgcgtcatgttaatttacaggtagtaaaatgtgtaatcgtggct
agccgtggatttgtgaaagacgcatttatgcagcatttaatagcacatgcagatgcaaat
gggaagaaatttacaacggaacaaagggcaaaattcatgttaactcattctagtagtgga
ttcaaacatgcattgaaagaagttttagagactcctcaagttgcgttgaggttagctgat
acaaaggcgcaaggagaagtcaaagcgttgaatcaatttttggaattaatgtcaacagaa
cctgatcgtgcattttatggcttcaaccatgtgaatcgtgcaaatcaagagctcgccatc
gaaacactccttgtcgctgattctctattccgagcccaagatattgagacacgacgaaaa
tacgtaagacttgtggaaagtgttcgagaacagaacggaaaagttcacattttctcttca
atgcacgtttctggagaacaactcgctcagttgactggatgtgcagctattttgagattt
cc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCAGTACGGCTTTCATGCGTCATG 412 465 577 Antisense
AATGTGTAATCGTGGCTAGCCGTGG 21 163 620 Antisense
TTAGAGACTCCTCAAGTTGCGTTGA 585 679 781 Antisense
GATCGTGCATTTTATGGCTTCAACC 491 417 880 Antisense
GGCTTCAACCATGTGAATCGTGCAA 569 537 895 Antisense
CGTGCAAATCAAGAGCTCGCCATCG 693 243 913 Antisense
ATCGAAACACTCCTTGTCGCTGATT 228 37 934 Antisense
TTCTCTATTCCGAGCCCAAGATATT 452 691 957 Antisense
TTTTCTCTTCAATGCACGTTTCTGG 253 677 1046 Antisense
GTTTCTGGAGAACAACTCGCTCAGT 659 449 1063 Antisense
GATGTGCAGCTATTTTGAGATTTCC 345 409 1094 Antisense
Sequence Source Affymetrix Proprietary Database