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[189047_at] cytochrome B5

Description W02D3.1 /REP_DB=WormBase Gene ID /WP=CE14418 /TR=O01816 /GB=AAK21480.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=cytochrome B5
Cluster 6grp_highest_10_10 > 11      6grp_high1_10_10 > 01      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 05      6grp_total > 08      4grp_total > 04      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11152
1 2 3 4 range
A 7240.2
(-2)
P 		5873.9
(-2.8)
P 		5624.6
(-3)
P 		15399
(3)
P 		9774
B 7798.4
(-1.6)
P 		5513.3
(-3)
P 		6070.8
(-2.7)
P 		15467.3
(3)
P 		9954
C 7601
(-1.8)
P 		5410.9
(-3)
P 		5586.7
(-2.9)
P 		16562.7
(3)
P 		11152
avr 7546.5
(-1.9) 		5599.4
(-3) 		5760.7
(-2.9) 		15809.7
(3) 		10210

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3757
1 2 3 4 5 6 range
A 9342.7
(3)
P 		6793.7
(-1.2)
P 		7722.7
(0.3)
P 		6784.4
(-1.3)
P 		5732.3
(-3)
P 		5852.7
(-2.8)
P 		3610
B 7759.7
(3)
P 		6987.7
(0.9)
P 		7201.9
(1.5)
P 		6320.4
(-1)
P 		5585.7
(-3)
P 		5886.8
(-2.2)
P 		2174
C 8390.6
(3)
P 		6500.4
(-1.8)
P 		6557.1
(-1.7)
P 		7269.2
(0.1)
P 		6610.4
(-1.6)
P 		6049.3
(-3)
P 		2341
avr 8497.7
(3) 		6760.6
(-1.1) 		7160.6
(-0.1) 		6791.3
(-1) 		5976.1
(-2.9) 		5929.6
(-3) 		2568
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2021
1 2 3 4 range
A 2636.2
(3)
P 		1275.1
(-3)
P 		1470.4
(-2.1)
P 		2254.1
(1.3)
P 		1361
B 2486.9
(0.5)
P 		1361.7
(-3)
P 		1406.7
(-2.9)
P 		3296.1
(3)
P 		1934
C 2516.2
(1.7)
P 		1555.7
(-3)
P 		1947.1
(-1.1)
P 		2792.7
(3)
P 		1237
avr 2546.4
(2) 		1397.5
(-3) 		1608.1
(-2.1) 		2781
(3) 		1384

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1685
1 2 3 4 5 6 range
A 1722.2
(-1.2)
P 		2416.2
(3)
P 		1681.8
(-1.4)
P 		1720.7
(-1.2)
P 		1729.8
(-1.2)
P 		1425
(-3)
P 		991
B 2700
(3)
P 		2325.9
(0.8)
P 		1748.8
(-2.6)
P 		1674.1
(-3)
P 		2269.4
(0.5)
P 		2195.2
(0)
P 		1026
C 3110
(3)
P 		1983.5
(-1.1)
P 		1668.9
(-2.2)
P 		1461.9
(-3)
P 		2139
(-0.5)
P 		2025.1
(-0.9)
P 		1648
avr 2510.7
(3) 		2241.9
(1.2) 		1699.8
(-2.5) 		1618.9
(-3) 		2046.1
(-0.1) 		1881.8
(-1.2) 		892
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189047_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1922
Sequence Type Exemplar sequence
Representative Public ID W02D3.1 NCBI
Target Description W02D3.1 /REP_DB=WormBase Gene ID /WP=CE14418 /TR=O01816 /GB=AAK21480.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=cytochrome B5
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059530(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059530 NCBI Caenorhabditis elegans W02D3.1 (W02D3.1) mRNA, complete cds. 11/11 None
SNAP00000042721 ENSEMBL cdna:SNAP chromosome:CEL140:I:6749422:6749898:1 11/11 None
GENEFINDER00000042726 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6749422:6749898:1 11/11 None
W02D3.1.2 ENSEMBL cdna:known chromosome:CEL140:I:6749408:6750004:1 gene:W02D3.1 11/11 None
W02D3.1.1 ENSEMBL cdna:known chromosome:CEL140:I:6749410:6750110:1 gene:W02D3.1 11/11 None
W02D3.1.3 ENSEMBL cdna:known chromosome:CEL140:I:6749410:6750014:1 gene:W02D3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6749350-6749827(+) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome B5
Ensembl W02D3.1
Entrez Gene 172393 Entrez gene
SwissProt O01816 EMBL-EBI
WormBase CE14418 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:248217_AT cytochrome b5 isoform 1 at
CANINE:1601591_AT similar to Cytochrome b5 cfa
CANINE:1587541_AT similar to Cytochrome b5 cfa
CANINE_2:CFA.15282.1.S1_AT similar to Cytochrome b5 cfa
CANINE_2:CFAAFFX.11724.1.S1_S_AT similar to Cytochrome b5 cfa
DROSOPHILA_2:1634641_A_AT dm
DROSGENOME1:146746_AT dm
CHICKEN:GGA.4989.1.S1_S_AT cytochrome b-5 gga
CHICKEN:GGAAFFX.21828.1.S1_S_AT cytochrome b-5 gga
HG-U95AV2:38460_AT cytochrome b-5 hs
HG-U133_PLUS_2:207843_X_AT cytochrome b-5 hs
HG-U133A:207843_X_AT cytochrome b-5 hs
HG-U133A_2:207843_X_AT cytochrome b-5 hs
HUGENEFL:M22976_AT cytochrome b-5 hs
HG-U133A:209366_X_AT cytochrome b-5 hs
HG-U133_PLUS_2:209366_X_AT cytochrome b-5 hs
HG-U133A_2:209366_X_AT cytochrome b-5 hs
U133_X3P:G181226_3P_X_AT cytochrome b-5 hs
U133_X3P:G181226_3P_A_AT cytochrome b-5 hs
U133_X3P:HS.83834.2.A1_3P_X_AT cytochrome b-5 hs
U133_X3P:HS.83834.2.A1_3P_AT cytochrome b-5 hs
U133_X3P:HS.83834.2.A1_3P_A_AT cytochrome b-5 hs
U133_X3P:HS.302116.0.S1_3P_AT cytochrome b-5 hs
HG-U133A_2:215726_S_AT cytochrome b-5 hs
HG-FOCUS:215726_S_AT cytochrome b-5 hs
HG-U133_PLUS_2:215726_S_AT cytochrome b-5 hs
HG-U133A:215726_S_AT cytochrome b-5 hs
HG-U133A_2:217021_AT cytochrome b-5 hs
HG-U133_PLUS_2:217021_AT cytochrome b-5 hs
HG-U133A:217021_AT cytochrome b-5 hs
HG-U95AV2:38458_AT cytochrome b-5 hs
HG-U95AV2:38459_G_AT cytochrome b-5 hs
HG-U95C:66325_AT Cytochrome b-5 hs
HG-U95D:76175_AT Cytochrome b-5 hs
MG-U74AV2:98533_AT cytochrome b-5 mm
MOE430A:1416727_A_AT cytochrome b-5 mm
MOUSE430A_2:1416727_A_AT cytochrome b-5 mm
MOUSE430_2:1416727_A_AT cytochrome b-5 mm
MU19KSUBC:TC40672_AT cytochrome b-5 mm
MU19KSUBC:TC40672_G_AT cytochrome b-5 mm
MG-U74BV2:115979_AT Cytochrome b-5, mRNA (cDNA clone MGC:35713 IMAGE:4973059) mm
MU19KSUBB:TC26793_AT Cytochrome b-5, mRNA (cDNA clone MGC:35713 IMAGE:4973059) mm
MU19KSUBB:TC26793_G_AT Cytochrome b-5, mRNA (cDNA clone MGC:35713 IMAGE:4973059) mm
MU19KSUBC:TC40671_S_AT Cytochrome b-5, mRNA (cDNA clone MGC:35713 IMAGE:4973059) mm
RAE230A:1386904_A_AT cytochrome b-5 rn
RAT230_2:1386904_A_AT cytochrome b-5 rn
RT-U34:AF007107_S_AT cytochrome b-5 rn
RG-U34A:AF007107_S_AT cytochrome b-5 rn
RT-U34:RC_AA817685_AT cytochrome b-5 rn
RG-U34A:RC_AA817685_AT cytochrome b-5 rn
RT-U34:RC_AA945054_S_AT cytochrome b-5 rn
RG-U34A:RC_AA945054_S_AT cytochrome b-5 rn
RAE230B:1396399_AT Cytochrome b-5 rn
RAT230_2:1396399_AT Cytochrome b-5 rn
Protein Similarities
Method ID Description E-Value
blast AAK21480 Hypothetical protein W02D3.1 [Caenorhabditis elegans] ref|NP_491931.1| W02D3.1 [Caenorhabditis elegans] 3.0E-77
blast CAE60440 Hypothetical protein CBG04048 [Caenorhabditis briggsae] 9.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR001199 EMBL-EBI Cytochrome b5 1.1E-22
Sequence
Target Sequence 
>C. ELEGANS:189047_AT
tcggagctacgtgtcatttctctcgatgaggtctcaaagcacaactgggaagatgctgat
caatcgtgttggattgtcatctccggaaaggtctacgatgtgacaaagttcttgaatgag
catccaggaggtgaagaagtcatcactcaattagccggtaaagatgctacagttggattc
ctcgatgttgggcactcaaaagatgctattgaaatggccaatgaatatctcattggtcaa
ttgccagaatcagacgttccaaaagtcgagacagcggctgcgaaaccttcgaaaaacgaa
aaatcatcatctctcctcaatgatttcaccgaaattatgacttcaccaacatggaccaac
ttcctgattccaactacaatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGGAGCTACGTGTCATTTCTCTCG 280 607 16 Antisense
ATCGTGTTGGATTGTCATCTCCGGA 351 35 78 Antisense
GAAGTCATCACTCAATTAGCCGGTA 299 335 151 Antisense
AAGATGCTACAGTTGGATTCCTCGA 216 155 176 Antisense
TGGATTCCTCGATGTTGGGCACTCA 163 551 189 Antisense
CAATTGCCAGAATCAGACGTTCCAA 503 179 253 Antisense
CGAGACAGCGGCTGCGAAACCTTCG 565 249 282 Antisense
ACGAAAAATCATCATCTCTCCTCAA 202 93 311 Antisense
CTCTCCTCAATGATTTCACCGAAAT 297 231 326 Antisense
TTCACCAACATGGACCAACTTCCTG 203 687 357 Antisense
CAACTTCCTGATTCCAACTACAATG 188 189 372 Antisense
Sequence Source Affymetrix Proprietary Database