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[189039_at] insulinase

Description C28F5.4 /REP_DB=WormBase Gene ID /WP=CE01818 /TR=SW:Q10040 /GB=AAC46729.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=insulinase
Cluster 6grp_total > 10      4grp_total > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 41.2
(1.1)
A 		38.2
(0.5)
A 		23.3
(-3)
A 		49.2
(3)
A 		26
B 3.7
(-3)
A 		10.2
(-2.2)
A 		5
(-2.8)
A 		52.2
(3)
A 		49
C 43.4
(-0.7)
A 		6.7
(-3)
A 		30
(-1.5)
A 		102.3
(3)
A 		96
avr 29.4
(-1.7) 		18.4
(-3) 		19.4
(-2.9) 		67.9
(3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 17.3
(-1.4)
A 		4.7
(-3)
A 		50.8
(3)
A 		6
(-2.8)
A 		25.1
(-0.3)
A 		24.6
(-0.4)
A 		46
B 24.8
(-2.9)
A 		45.9
(3)
A 		30.5
(-1.3)
A 		24.3
(-3)
A 		40.6
(1.5)
A 		38
(0.8)
A 		22
C 2.5
(-3)
A 		51.1
(3)
A 		9.2
(-2.2)
A 		3.7
(-2.9)
A 		9.6
(-2.1)
A 		6.2
(-2.5)
A 		49
avr 14.9
(-2) 		33.9
(3) 		30.2
(2) 		11.3
(-3) 		25.1
(0.7) 		22.9
(0.1) 		23
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 15
(3)
A 		8.3
(-1.5)
A 		6.1
(-3)
A 		7.2
(-2.3)
A 		9
B 1.2
(-3)
A 		2.5
(-2.2)
A 		1.2
(-3)
A 		11.1
(3)
A 		10
C 14.4
(1.9)
A 		1.9
(-3)
A 		10.5
(0.4)
A 		17.2
(3)
A 		15
avr 10.2
(1.7) 		4.2
(-3) 		5.9
(-1.7) 		11.8
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 3.2
(-1.9)
A 		1.7
(-2.9)
A 		11.1
(3)
A 		1.5
(-3)
A 		7.6
(0.8)
A 		6
(-0.2)
A 		10
B 8.6
(-1.7)
A 		15.3
(2.3)
A 		7.4
(-2.4)
A 		6.4
(-3)
A 		16.5
(3)
A 		14.2
(1.6)
A 		10
C 0.9
(-2.9)
A 		15.6
(3)
A 		2.3
(-2.4)
A 		0.7
(-3)
A 		3.1
(-2)
A 		2.1
(-2.4)
A 		15
avr 4.2
(-2) 		10.9
(3) 		6.9
(0) 		2.9
(-3) 		9.1
(1.7) 		7.4
(0.4) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189039_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4154
Sequence Type Exemplar sequence
Representative Public ID C28F5.4 NCBI
Target Description C28F5.4 /REP_DB=WormBase Gene ID /WP=CE01818 /TR=SW:Q10040 /GB=AAC46729.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=insulinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063174(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063174 NCBI Caenorhabditis elegans C28F5.4 (insulinase) mRNA, complete cds. 11/11 None
SNAP00000012810 ENSEMBL cdna:SNAP chromosome:CEL140:II:7520043:7521731:-1 10/11 None
C28F5.4 ENSEMBL cdna:known chromosome:CEL140:II:7520104:7523292:-1 gene:C28F5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7520100-7523289(-) 100.0 100.0
Public Domain and Genome References
Gene Title insulinase
Ensembl C28F5.4
Entrez Gene 182987 Entrez gene
SwissProt Q10040 EMBL-EBI
WormBase CE01818 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4222 metalloendopeptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC46729 Hypothetical protein C28F5.4 [Caenorhabditis elegans] ref|NP_495575.1| C28F5.4 [Caenorhabditis elegans] sp|Q10040|YQA4_CAEEL Hypothetical zinc protease C28F5.4 0.0
blast AAM45374 Hypothetical protein F44E7.4b [Caenorhabditis elegans] ref|NP_741542.1| F44E7.4b [Caenorhabditis elegans] 1.0E-177
blast AAO12429 Hypothetical protein F44E7.4c [Caenorhabditis elegans] ref|NP_504514.2| F44E7.4c [Caenorhabditis elegans] 1.0E-174
blast AAT92074 Hypothetical protein F44E7.4d [Caenorhabditis elegans] ref|NP_001023928.1| F44E7.4d [Caenorhabditis elegans] 1.0E-172
blast AAO12429 Hypothetical protein F44E7.4c [Caenorhabditis elegans] ref|NP_504514.2| F44E7.4c [Caenorhabditis elegans] 1.0E-172
Protein Domains
Database ID Description E-Value
Pfam IPR007863 EMBL-EBI Peptidase M16, C-terminal 1.6E-19
Pfam IPR007863 EMBL-EBI Peptidase M16, C-terminal 1.0E-30
Pfam IPR007863 EMBL-EBI Peptidase M16, C-terminal 1.6E-19
Pfam IPR001431 EMBL-EBI Peptidase M16, insulinase-like 1.6E-62
Sequence
Target Sequence 
>C. ELEGANS:189039_AT
gttgcaccaaattcacgtccattgaagcgtgatgagcacaatccccatcgggagttgcag
ttgattaatggtcatgaacatgtctacagacacttccagaagactcatgatgttggatgc
gtggaagtcgcttttcaaattggagttcagagcacctacaacaattccgttaataaattg
ctcaatgaactaataaaaaatccagcgtacaccattctcaggactaatgaagctcttggt
tataatgtatccactgaatcacgtctaaacgatggaaatgtttacctccacgttattgtc
caagggccggagagtgcggaccacgttctcgagagaattgaggtattcttggagtctgct
cgagaagaaattgtcgcaatgccacaagaagatttcgattatcaagtttgggcgatgttt
aaagaaaatccgccgactttgagccaatgctttagcatgttctggagtgagattcattct
agacagtacaatttcggacgcaataaagaagtgcgtggcatttcaaaaagaatcacgaaa
gaggaagtcatcaatttttttgacaggtatcacacttgccaaatta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTGCACCAAATTCACGTCCATTGA 572 439 1633 Antisense
GAAGCGTGATGAGCACAATCCCCAT 349 343 1656 Antisense
ATCCCCATCGGGAGTTGCAGTTGAT 471 33 1673 Antisense
TCAGAGCACCTACAACAATTCCGTT 373 623 1779 Antisense
AAAAAATCCAGCGTACACCATTCTC 634 129 1827 Antisense
GTATCCACTGAATCACGTCTAAACG 535 453 1879 Antisense
GAAATGTTTACCTCCACGTTATTGT 191 359 1907 Antisense
CAAGGGCCGGAGAGTGCGGACCACG 698 159 1933 Antisense
GAGGTATTCTTGGAGTCTGCTCGAG 84 403 1972 Antisense
GCCGACTTTGAGCCAATGCTTTAGC 308 285 2064 Antisense
GACAGGTATCACACTTGCCAAATTA 438 335 2194 Antisense
Sequence Source Affymetrix Proprietary Database