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[189036_s_at]

Description ZK256.1A /REP_DB=WormBase Gene ID /WP=CE27556 /TR=Q9XTG4 /GB=CAB05000.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=E1-E2 ATPases
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 27      4grp_highest_8_8 > 42      4grp_high_8_8 > 48      6grp_highest_nonorm > 60      6grp_total > 27      4grp_total > 55      6grp_highest_sub > De      6grp_cgc > 13      6grp_high1_sub > Cu      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 220
1 2 3 4 range
A 486.1
(3)
P 		377.1
(-1)
P 		393.5
(-0.4)
P 		321.4
(-3)
P 		165
B 452.3
(3)
P 		266.5
(-3)
P 		378.3
(0.6)
P 		270
(-2.9)
P 		186
C 466.3
(3)
P 		312.2
(-2.7)
P 		328.7
(-2.1)
P 		304.3
(-3)
P 		162
avr 468.2
(3) 		318.6
(-2.3) 		366.8
(-0.6) 		298.6
(-3) 		170

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 425
1 2 3 4 5 6 range
A 481.4
(3)
P 		410.7
(1.2)
P 		248.7
(-3)
P 		345.4
(-0.5)
P 		335.4
(-0.8)
P 		395.4
(0.8)
P 		233
B 465.9
(3)
P 		413.2
(1.5)
P 		247.9
(-3)
P 		326.9
(-0.8)
P 		304
(-1.5)
P 		370.3
(0.4)
P 		218
C 672.6
(3)
P 		435.1
(-1)
P 		437.9
(-0.9)
P 		380.5
(-1.9)
P 		356.5
(-2.3)
P 		313.6
(-3)
P 		359
avr 540
(3) 		419.7
(-0.2) 		311.5
(-3) 		350.9
(-2) 		332
(-2.5) 		359.8
(-1.7) 		229
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 130
1 2 3 4 range
A 177
(3)
P 		81.9
(-1.4)
P 		102.9
(-0.4)
P 		47
(-3)
P 		130
B 144.2
(3)
P 		65.8
(-2.4)
P 		87.7
(-0.9)
P 		57.5
(-3)
P 		87
C 154.3
(3)
P 		89.8
(-0.8)
P 		114.6
(0.7)
P 		51.3
(-3)
P 		103
avr 158.5
(3) 		79.2
(-1.5) 		101.7
(-0.2) 		51.9
(-3) 		107

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 195
1 2 3 4 5 6 range
A 88.7
(-0.7)
P 		146.1
(3)
P 		54.2
(-3)
P 		87.6
(-0.8)
P 		101.2
(0.1)
P 		96.3
(-0.3)
P 		92
B 162.1
(3)
P 		137.5
(1.6)
P 		60.2
(-3)
P 		86.6
(-1.4)
P 		123.5
(0.7)
P 		138.1
(1.6)
P 		102
C 249.3
(3)
P 		132.7
(-1)
P 		111.5
(-1.8)
P 		76.5
(-3)
P 		115.3
(-1.7)
P 		105
(-2)
P 		173
avr 166.7
(3) 		138.8
(1.2) 		75.3
(-3) 		83.6
(-2.5) 		113.3
(-0.5) 		113.1
(-0.5) 		91
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189036_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.784
Sequence Type Exemplar sequence
Representative Public ID ZK256.1A NCBI
Target Description ZK256.1A /REP_DB=WormBase Gene ID /WP=CE27556 /TR=Q9XTG4 /GB=CAB05000.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=E1-E2 ATPases
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026689(10), NM_001026691(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026689 NCBI Caenorhabditis elegans PMR-type Golgi ATPase family member (pmr-1) (pmr-1) mRNA, complete cds. 10/11 None
NM_001026691 NCBI Caenorhabditis elegans PMR-type Golgi ATPase family member (pmr-1) (pmr-1) mRNA, complete cds. 10/11 None
SNAP00000015700 ENSEMBL cdna:SNAP chromosome:CEL140:I:13028616:13032350:1 10/11 None
GENEFINDER00000015701 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:13028688:13031412:1 10/11 None
ZK256.1a ENSEMBL cdna:known chromosome:CEL140:I:13012557:13031740:1 gene:ZK256.1 10/11 A 
ZK256.1c ENSEMBL cdna:known chromosome:CEL140:I:13019550:13031740:1 gene:ZK256.1 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173176 3 2 NM_001026689 189036_s_at (10)
NM_001026691 189036_s_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13020270-13031343(+) 99.06 100.0
Public Domain and Genome References
Ensembl ZK256.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAF32230 Hypothetical protein ZK256.1c [Caenorhabditis elegans] emb|CAF32231.1| Hypothetical protein ZK256.1c [Caenorhabditis elegans] ref|NP_001021862.1| PMR-type Golgi ATPase family member (pmr-1) [Caenorhabditis elegans] 0.0
blast CAB05000 Hypothetical protein ZK256.1a [Caenorhabditis elegans] emb|CAB04015.2| Hypothetical protein ZK256.1a [Caenorhabditis elegans] emb|CAC19896.1| PMR1 protein [Caenorhabditis elegans] emb|CAC19895.1| PMR1 protein [Caenorhabditis elegans] ref|NP_001021860.1| PMR-type Golgi ATPase family member (pmr-1) [Caenorhabditis elegans] 0.0
blast CAE63458 Hypothetical protein CBG07921 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A1A3_HUMAN (P13637) Sodium/potassium-transporting ATPase alpha-3 chain (EC 3.6.3.9) (Sodium pump 3) (Na+/K+ ATPase 3) (Alpha(III)) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 1.9E-50
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 7.7E-100
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 2.0E-25
Pfam IPR004014 EMBL-EBI Cation transporting ATPase, N-terminal 1.1E-26
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 1.9E-50
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 7.7E-100
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 2.0E-25
Pfam IPR004014 EMBL-EBI Cation transporting ATPase, N-terminal 1.1E-26
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 1.9E-50
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 1.9E-50
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021860.1 8 65-82,87-104,255-272,287-309,757-779,794-811,832-854,858-877
NP_001021862.1 7 163-180,332-349,364-386,834-856,871-888,909-931,935-954
ZK256.1a 8 65-82,87-104,255-272,287-309,757-779,794-811,832-854,858-877
ZK256.1c 7 163-180,332-349,364-386,834-856,871-888,909-931,935-954
SNAP00000015700 4 112-134,149-166,187-209,213-232
GENEFINDER00000015701 4 88-110,125-142,163-185,189-208
Sequence
Target Sequence 
>C. ELEGANS:189036_S_AT
accaccggcacaatcacttggagttgaaccagttgatgatgatattattaggcaacgacc
acgaaatacgaaacaaccaatgttaaccggaaaattgattgccgatattctcgcgtctgc
cgcgattattgttgtcggcacgttgagtgtgttttataaagagatgtcagccgacaataa
agtaacaccacgtgacacgacaatgacattcacctgttttgtcctattcgatatgtggaa
tgccctttcatgcagatcatcacgtaaaatgatatggcaaatcggactgcgacggaatcg
aatgttctcgttggctgtgtctgcctcacttatctgccagcttctcgtcatctactgggc
accacttcaacacatttttcagactgaagcactttctctttttggtacggaaattgttga
aaatgcactgatttttgagctaaatttgaaaaaagttctgaattctaatctcatcttcct
gacaacaatcacatcgtctgtgttcattttcaacgaaactcggaaatatttctcacttcg
ctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCACCGGCACAATCACTTGGAGTT 498 85 2196 Antisense
ATATTCTCGCGTCTGCCGCGATTAT 320 19 2300 Antisense
GATTATTGTTGTCGGCACGTTGAGT 398 429 2319 Antisense
ATTCACCTGTTTTGTCCTATTCGAT 56 13 2403 Antisense
GGAATGCCCTTTCATGCAGATCATC 87 533 2432 Antisense
GGCAAATCGGACTGCGACGGAATCG 701 531 2471 Antisense
TCGAATGTTCTCGTTGGCTGTGTCT 477 601 2493 Antisense
GACTGAAGCACTTTCTCTTTTTGGT 456 373 2577 Antisense
TCTCATCTTCCTGACAACAATCACA 450 613 2664 Antisense
AATCACATCGTCTGTGTTCATTTTC 47 171 2682 Antisense
TCGGAAATATTTCTCACTTCGCTCC 50 607 2715 Antisense
Sequence Source Affymetrix Proprietary Database