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[189033_at] 7TM chemoreceptor, srg family

Description T12A2.9 /REP_DB=WormBase Gene ID /WP=CE01402 /GEN=srg-8 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=seven-transmembrane receptor
Cluster 6grp_total > 81      4grp_total > 19      6grp_cgc > 84      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 104
1 2 3 4 range
A 36.1
(-2)
A 		22.9
(-2.9)
A 		21.9
(-3)
A 		107.9
(3)
A 		86
B 32.5
(-0.1)
A 		62.5
(3)
A 		4.4
(-3)
A 		62.4
(3)
P 		58
C 24.9
(-3)
P 		68.4
(2)
P 		27.1
(-2.7)
A 		76.6
(3)
A 		52
avr 31.2
(-1.8) 		51.3
(0.1) 		17.8
(-3) 		82.3
(3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 38.7
(1.6)
A 		32
(0.7)
A 		48.4
(3)
M 		6.3
(-3)
A 		40.9
(1.9)
A 		13.8
(-1.9)
A 		42
B 21.2
(-3)
A 		37.1
(0.5)
A 		34.2
(-0.1)
A 		48.2
(3)
A 		32.6
(-0.5)
A 		30.5
(-0.9)
A 		27
C 23.8
(-1.6)
A 		34.3
(-0.3)
P 		12.1
(-3)
A 		62.2
(3)
P 		27.4
(-1.2)
A 		13.1
(-2.9)
A 		50
avr 27.9
(-0.3) 		34.5
(1.7) 		31.6
(0.8) 		38.9
(3) 		33.6
(1.4) 		19.1
(-3) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 13.2
(1.6)
A 		5
(-3)
A 		5.7
(-2.6)
A 		15.8
(3)
A 		11
B 10.4
(0.9)
A 		15.4
(3)
A 		1
(-3)
A 		13.3
(2.1)
P 		14
C 8.2
(-3)
P 		19.7
(3)
P 		9.4
(-2.4)
A 		12.9
(-0.5)
A 		12
avr 10.6
(0.6) 		13.4
(2.6) 		5.4
(-3) 		14
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 7.1
(0.1)
A 		11.4
(2.5)
A 		10.5
(2)
M 		1.6
(-3)
A 		12.3
(3)
A 		3.4
(-2)
A 		11
B 7.4
(-3)
A 		12.3
(2)
A 		8.3
(-2.1)
A 		12.8
(2.5)
A 		13.3
(3)
A 		11.4
(1.1)
A 		6
C 8.8
(0.6)
A 		10.5
(1.7)
P 		3.1
(-3)
A 		12.5
(3)
P 		8.9
(0.7)
A 		4.4
(-2.2)
A 		9
avr 7.8
(-1.4) 		11.4
(2.9) 		7.3
(-1.9) 		9
(0.1) 		11.5
(3) 		6.4
(-3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189033_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6311
Sequence Type Exemplar sequence
Representative Public ID T12A2.9 NCBI
Target Description T12A2.9 /REP_DB=WormBase Gene ID /WP=CE01402 /GEN=srg-8 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=seven-transmembrane receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065963(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065963 NCBI Caenorhabditis elegans Serpentine Receptor, class G (gamma) family member (srg-8) (srg-8) mRNA, complete cds. 11/11 None
T12A2.9 ENSEMBL cdna:known chromosome:CEL140:III:6253492:6254776:-1 gene:T12A2.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000026129 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6253490-6254775(-) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, srg family
Gene Symbol srg-8
Ensembl T12A2.9
Entrez Gene 191834 Entrez gene
SwissProt P46565 EMBL-EBI
WormBase CE01402 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7165 signal transduction inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4888 transmembrane receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC24448 Serpentine receptor, class g (gamma) protein 8 [Caenorhabditis elegans] ref|NP_498364.1| Serpentine Receptor, class G (gamma) family member (srg-8) [Caenorhabditis elegans] sp|P46565|SRG8_CAEEL Serpentine receptor class gamma-8 (Protein srg-8) 1.0E-178
blast 2123261AH chemosensory receptor 1.0E-176
blast CAE70739 Hypothetical protein CBG17484 [Caenorhabditis briggsae] 1.0E-113
Protein Domains
Database ID Description E-Value
Pfam IPR000609 EMBL-EBI Integral membrane protein Srg, nematode type 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498364.1 7 27-49,61-83,103-125,145-167,187-209,230-252,267-289
T12A2.9 7 27-49,61-83,103-125,145-167,187-209,230-252,267-289
Sequence
Target Sequence 
>C. ELEGANS:189033_AT
tcaaatctttctcaccacaaatcgaatgtcatctgtattatggcctgtagatcacgagaa
attttggaagataaacttttcgagaatattaattctgaatttgattgctccgttcttctt
catttggaatacaataatatcgaagaaagttttaatattttactttggaggattctacat
caactacttaaaaataatcccatgggccagtatgtctctgtttctatttataattcggtc
agcagtagttatgattactgtagttactacatcaataacattttggaaaatgtcccatat
gaagaacagattgaaaaaatcagagggaaccttgtgtaaagcatgcgcagcaaattcgat
ttgttttttggttcctgctgtttttgaggctatgaaagttcttaatactgattggggaaa
acattggttggcttatcttgtgcagccatttgcatgggatgttctaaatgttggatcccc
gttggttatgatttttgcaagtggacaactcagaactcatgcatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAAATCTTTCTCACCACAAATCGA 463 625 375 Antisense
GAATGTCATCTGTATTATGGCCTGT 462 331 398 Antisense
TGAATTTGATTGCTCCGTTCTTCTT 3 577 470 Antisense
GCTCCGTTCTTCTTCATTTGGAATA 414 299 481 Antisense
TGGGCCAGTATGTCTCTGTTTCTAT 134 555 577 Antisense
GAACCTTGTGTAAAGCATGCGCAGC 292 345 701 Antisense
GATTTGTTTTTTGGTTCCTGCTGTT 459 427 732 Antisense
ACATTGGTTGGCTTATCTTGTGCAG 155 105 795 Antisense
ATGTTCTAAATGTTGGATCCCCGTT 439 47 833 Antisense
GATCCCCGTTGGTTATGATTTTTGC 28 417 848 Antisense
GTGGACAACTCAGAACTCATGCATT 430 491 875 Antisense
Sequence Source Affymetrix Proprietary Database