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[189017_at] glutaminase

Description F30F8.2 /REP_DB=WormBase Gene ID /WP=CE09814 /TR=SW:Q93650 /GB=CAB03030.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=glutaminase
Cluster 6grp_high1_10_10 > 58      6grp_total > 47      4grp_total > 22      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 233
1 2 3 4 range
A 229
(0.3)
P 		135.1
(-2.1)
A 		99.6
(-3)
A 		333
(3)
P 		233
B 246.7
(2.6)
P 		111.5
(-3)
A 		134.8
(-2)
A 		256.6
(3)
P 		145
C 319.4
(3)
P 		135.4
(-2.9)
A 		133.5
(-3)
P 		194.4
(-1)
P 		186
avr 265
(3) 		127.3
(-2.8) 		122.6
(-3) 		261.3
(2.8) 		142

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 205.3
(0.4)
P 		159.7
(-1.7)
P 		133.2
(-3)
P 		137.1
(-2.8)
A 		160.9
(-1.7)
P 		258.8
(3)
P 		126
B 202.6
(1.5)
P 		222.4
(3)
P 		179
(-0.2)
P 		140.5
(-3)
P 		162.1
(-1.4)
P 		169.2
(-0.9)
P 		82
C 163.1
(-1.5)
P 		226.2
(3)
P 		158.1
(-1.9)
P 		148.8
(-2.6)
P 		142.8
(-3)
P 		214.2
(2.1)
P 		83
avr 190.3
(1) 		202.8
(2.1) 		156.8
(-1.8) 		142.1
(-3) 		155.3
(-1.9) 		214.1
(3) 		72
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 83.4
(3)
P 		29.3
(-2.7)
A 		26
(-3)
A 		48.7
(-0.6)
P 		57
B 78.7
(3)
P 		27.5
(-3)
A 		31.2
(-2.6)
A 		54.7
(0.2)
P 		51
C 105.7
(3)
P 		38.9
(-2.5)
A 		46.5
(-1.9)
P 		32.8
(-3)
P 		73
avr 89.3
(3) 		31.9
(-3) 		34.6
(-2.7) 		45.4
(-1.6) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 37.8
(-1.4)
P 		56.8
(1.9)
P 		29
(-3)
P 		34.8
(-2)
A 		48.5
(0.4)
P 		63
(3)
P 		34
B 70.5
(2.4)
P 		74
(3)
P 		43.5
(-2)
P 		37.2
(-3)
P 		65.9
(1.7)
P 		63.1
(1.2)
P 		37
C 60.4
(1.4)
P 		69
(2.6)
P 		40.2
(-1.5)
P 		29.9
(-3)
P 		46.2
(-0.7)
P 		71.7
(3)
P 		42
avr 56.2
(1.1) 		66.6
(3) 		37.6
(-2.3) 		34
(-3) 		53.5
(0.6) 		65.9
(2.9) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189017_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1023
Sequence Type Exemplar sequence
Representative Public ID F30F8.2 NCBI
Target Description F30F8.2 /REP_DB=WormBase Gene ID /WP=CE09814 /TR=SW:Q93650 /GB=CAB03030.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=glutaminase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059761(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059761 NCBI Caenorhabditis elegans F30F8.2 (glutaminase) mRNA, complete cds. 9/11 None
GENEFINDER00000014216 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7835201:7839652:-1 11/11 None
SNAP00000014200 ENSEMBL cdna:SNAP chromosome:CEL140:I:7835402:7838011:-1 9/11 None
F30F8.2 ENSEMBL cdna:known chromosome:CEL140:I:7834573:7839755:-1 gene:F30F8.2 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7835131-7839583(-) 91.64 100.0
Public Domain and Genome References
Gene Title glutaminase
Ensembl F30F8.2
Entrez Gene 172547 Entrez gene
SwissProt Q93650 EMBL-EBI
WormBase CE28553 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6541 glutamine metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4359 glutaminase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAC70084 Hypothetical protein F30F8.2 [Caenorhabditis elegans] emb|CAB03030.2| Hypothetical protein F30F8.2 [Caenorhabditis elegans] ref|NP_492162.1| F30F8.2 [Caenorhabditis elegans] sp|Q93650|GLS2_CAEEL Putative glutaminase F30F8.2 (GLS) (L-glutamine amidohydrolase) 0.0
blast CAE56117 Hypothetical protein CBG23724 [Caenorhabditis briggsae] 0.0
blast T21575 hypothetical protein F30F8.2 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002110 EMBL-EBI Ankyrin 0.19
Pfam IPR002110 EMBL-EBI Ankyrin 0.043
Pfam IPR002110 EMBL-EBI Ankyrin 3.9
Pfam IPR007043 EMBL-EBI Glutaminase 1.0E-126
Pfam IPR007043 EMBL-EBI Glutaminase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:189017_AT
aagacactcttgatctctattttcaaatttgttctattgaaacaaactgtgattcattgg
ctgtgatggctgcaacattggcaaatggaggtgtcaatccgatgaatggagaacgaattg
tgaataatcgtgcttgccgtgatactctctcattgatgtactcttgtggaatgtacgatt
ggtcggggcagttcgctttccacgtaggtcttccagccaaatcaggagtatctggagata
tgattattgtgattccgaatgtgatgggtattgccttgtacagtccacgacttgataaac
ttggaaacacggttcgaggtgtcaagtttgcagaacagctcgtacaaaagtataacttcc
ataattatgatagtctggtaagttatctcaaaatagaaaattctaaagttaatttatttg
agttttctttcgaaatatcttatggtgggggcgcagagagaatctcctttagaaatcgac
ctgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGACACTCTTGATCTCTATTTTCA 505 153 1025 Antisense
GTGATTCATTGGCTGTGATGGCTGC 101 485 1073 Antisense
ATAATCGTGCTTGCCGTGATACTCT 688 25 1148 Antisense
GATACTCTCTCATTGATGTACTCTT 113 425 1165 Antisense
GTACGATTGGTCGGGGCAGTTCGCT 127 441 1197 Antisense
GTAGGTCTTCCAGCCAAATCAGGAG 215 457 1228 Antisense
GATGGGTATTGCCTTGTACAGTCCA 594 407 1287 Antisense
TGCCTTGTACAGTCCACGACTTGAT 303 585 1296 Antisense
GTCAAGTTTGCAGAACAGCTCGTAC 60 465 1345 Antisense
GGTGGGGGCGCAGAGAGAATCTCCT 373 499 1468 Antisense
GAATCTCCTTTAGAAATCGACCTGG 142 325 1484 Antisense
Sequence Source Affymetrix Proprietary Database