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[189016_at] alpha-adaptin

Description T20B5.1 /REP_DB=WormBase Gene ID /WP=CE02879 /TR=Q22601 /GB=AAA68332.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=alpha-adaptin
Cluster 6grp_highest_10_10 > 20      6grp_high1_10_10 > 05      4grp_highest_8_8 > 32      4grp_high_8_8 > 51      6grp_highest_nonorm > 40      6grp_total > 04      4grp_total > 52      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 686
1 2 3 4 range
A 892.3
(3)
P 		623.9
(0.2)
P 		623.6
(0.2)
P 		319.7
(-3)
P 		573
B 777
(3)
P 		774.4
(3)
P 		575.2
(0.6)
P 		263.1
(-3)
P 		514
C 890.4
(2.6)
P 		935.8
(3)
P 		887.5
(2.6)
P 		249.5
(-3)
P 		686
avr 853.2
(3) 		778
(2.2) 		695.4
(1.4) 		277.4
(-3) 		576

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 770
1 2 3 4 5 6 range
A 979.2
(3)
P 		935.2
(2.6)
P 		471.1
(-2)
P 		600.4
(-0.8)
P 		485.5
(-1.9)
P 		374.1
(-3)
P 		605
B 946.4
(3)
P 		760.6
(1.2)
P 		505.6
(-1.2)
P 		616.5
(-0.1)
P 		398.4
(-2.2)
P 		311.7
(-3)
P 		635
C 1081.8
(3)
P 		626.1
(-0.6)
P 		774.4
(0.6)
P 		589.6
(-0.9)
P 		682.8
(-0.1)
P 		319.6
(-3)
P 		762
avr 1002.5
(3) 		774
(0.9) 		583.7
(-0.8) 		602.2
(-0.6) 		522.2
(-1.3) 		335.1
(-3) 		667
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 283
1 2 3 4 range
A 324.9
(3)
P 		135.4
(-1.1)
P 		163
(-0.5)
P 		46.8
(-3)
P 		278
B 247.8
(3)
P 		191.3
(1.2)
P 		133.3
(-0.6)
P 		56.1
(-3)
P 		192
C 294.8
(2.7)
P 		269.1
(2.1)
P 		309.3
(3)
P 		42.1
(-3)
P 		267
avr 289.2
(3) 		198.6
(0.7) 		201.9
(0.8) 		48.3
(-3) 		241

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 310
1 2 3 4 5 6 range
A 180.5
(-0.8)
P 		332.6
(3)
P 		102.6
(-2.7)
P 		152.3
(-1.5)
P 		146.5
(-1.6)
P 		91.1
(-3)
P 		242
B 329.3
(3)
P 		253.2
(0.9)
P 		122.8
(-2.8)
P 		163.3
(-1.7)
P 		161.8
(-1.7)
P 		116.2
(-3)
P 		213
C 401
(3)
P 		191
(-1.3)
P 		197.1
(-1.2)
P 		118.6
(-2.8)
P 		220.9
(-0.7)
P 		107
(-3)
P 		294
avr 303.6
(3) 		258.9
(1.7) 		140.8
(-1.9) 		144.7
(-1.8) 		176.4
(-0.8) 		104.8
(-3) 		199
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189016_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16806
Sequence Type Exemplar sequence
Representative Public ID T20B5.1 NCBI
Target Description T20B5.1 /REP_DB=WormBase Gene ID /WP=CE02879 /TR=Q22601 /GB=AAA68332.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=alpha-adaptin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077171(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077171 NCBI Caenorhabditis elegans T20B5.1 (alpha-adaptin) mRNA, complete cds. 11/11 None
SNAP00000028853 ENSEMBL cdna:SNAP chromosome:CEL140:X:9338608:9342365:1 11/11 None
GENEFINDER00000028856 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9338608:9342365:1 11/11 None
T20B5.1.1 ENSEMBL cdna:known chromosome:CEL140:X:9336749:9342881:1 gene:T20B5.1 11/11 A 
T20B5.1.2 ENSEMBL cdna:known chromosome:CEL140:X:9338605:9342881:1 gene:T20B5.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9338607-9342365(+) 100.0 100.0
Public Domain and Genome References
Gene Title alpha-adaptin
Gene Symbol apa-2
Ensembl T20B5.1
Entrez Gene 181163 Entrez gene
SwissProt Q22601 EMBL-EBI
WormBase CE28680 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:249909_S_AT adaptin family protein at
CANINE:1583521_AT similar to Adapter-related protein complex 2 alpha 2 subunit (Alpha-adaptin C) (Adaptor protein complex AP-2 alpha-2 subunit) (Clathrin assembly protein complex 2 alpha-C large chain) (100 kDa coated vesicle protein C) (Plasma membrane adaptor HA2/... cfa
CANINE_2:CFA.17599.1.S1_S_AT similar to Adapter-related protein complex 2 alpha 2 subunit (Alpha-adaptin C) (Adaptor protein complex AP-2 alpha-2 subunit) (Clathrin assembly protein complex 2 alpha-C large chain) (100 kDa coated vesicle protein C) (Plasma membrane adaptor HA2/... cfa
CANINE_2:CFA.4099.1.A1_AT similar to Adapter-related protein complex 2 alpha 2 subunit (Alpha-adaptin C) (Adaptor protein complex AP-2 alpha-2 subunit) (Clathrin assembly protein complex 2 alpha-C large chain) (100 kDa coated vesicle protein C) (Plasma membrane adaptor HA2/... cfa
CANINE_2:CFA.4099.1.A1_S_AT similar to Adapter-related protein complex 2 alpha 2 subunit (Alpha-adaptin C) (Adaptor protein complex AP-2 alpha-2 subunit) (Clathrin assembly protein complex 2 alpha-C large chain) (100 kDa coated vesicle protein C) (Plasma membrane adaptor HA2/... cfa
DROSOPHILA_2:1634403_AT Adaptin dm
DROSGENOME1:143820_AT Adaptin dm
CHICKEN:GGA.1263.1.S1_AT adaptor-related protein complex 2, alpha 2 subunit gga
CHICKEN:GGA.13041.1.S1_AT adaptor-related protein complex 2, alpha 2 subunit gga
HG-U133_PLUS_2:215764_X_AT adaptor-related protein complex 2, alpha 2 subunit hs
HG-U133A_2:215764_X_AT adaptor-related protein complex 2, alpha 2 subunit hs
HG-U133A:215764_X_AT adaptor-related protein complex 2, alpha 2 subunit hs
HU35KSUBA:RC_AA281617_AT adaptor-related protein complex 2, alpha 2 subunit hs
HU35KSUBB:RC_H28956_S_AT adaptor-related protein complex 2, alpha 2 subunit hs
HG-U133A:211779_X_AT adaptor-related protein complex 2, alpha 2 subunit hs
HG-U133_PLUS_2:211779_X_AT adaptor-related protein complex 2, alpha 2 subunit hs
HG-U133A_2:211779_X_AT adaptor-related protein complex 2, alpha 2 subunit hs
U133_X3P:G13544040_3P_S_AT adaptor-related protein complex 2, alpha 2 subunit hs
U133_X3P:HS.19121.1.S1_3P_AT adaptor-related protein complex 2, alpha 2 subunit hs
U133_X3P:HS.19121.1.S1_3P_A_AT adaptor-related protein complex 2, alpha 2 subunit hs
U133_X3P:HS.19121.0.S2_3P_X_AT adaptor-related protein complex 2, alpha 2 subunit hs
U133_X3P:HS.19121.0.S2_3P_AT adaptor-related protein complex 2, alpha 2 subunit hs
U133_X3P:HS.19121.0.S2_3P_A_AT adaptor-related protein complex 2, alpha 2 subunit hs
U133_X3P:212161_3P_AT adaptor-related protein complex 2, alpha 2 subunit hs
U133_X3P:215764_3P_X_AT adaptor-related protein complex 2, alpha 2 subunit hs
HG-U133A_2:212159_X_AT adaptor-related protein complex 2, alpha 2 subunit hs
HG-U133_PLUS_2:212159_X_AT adaptor-related protein complex 2, alpha 2 subunit hs
HG-U133A:212159_X_AT adaptor-related protein complex 2, alpha 2 subunit hs
HG-U133A_2:212161_AT adaptor-related protein complex 2, alpha 2 subunit hs
HG-U133_PLUS_2:212161_AT adaptor-related protein complex 2, alpha 2 subunit hs
HG-U133A:212161_AT adaptor-related protein complex 2, alpha 2 subunit hs
HG-U95AV2:32228_AT adaptor-related protein complex 2, alpha 2 subunit hs
HU35KSUBB:RC_T93279_AT Adaptor-related protein complex 2, alpha 2 subunit hs
MOE430A:1432007_S_AT adaptor protein complex AP-2, alpha 2 subunit mm
MOUSE430_2:1432007_S_AT adaptor protein complex AP-2, alpha 2 subunit mm
MOUSE430A_2:1432007_S_AT adaptor protein complex AP-2, alpha 2 subunit mm
MOE430A:1432006_AT adaptor protein complex AP-2, alpha 2 subunit mm
MOUSE430_2:1432006_AT adaptor protein complex AP-2, alpha 2 subunit mm
MOUSE430A_2:1432006_AT adaptor protein complex AP-2, alpha 2 subunit mm
MOUSE430_2:1452490_A_AT adaptor protein complex AP-2, alpha 2 subunit mm
MOE430A:1452490_A_AT adaptor protein complex AP-2, alpha 2 subunit mm
MOUSE430A_2:1452490_A_AT adaptor protein complex AP-2, alpha 2 subunit mm
MU11KSUBA:AA000469_G_AT adaptor protein complex AP-2, alpha 2 subunit mm
MU11KSUBA:AA000469_AT adaptor protein complex AP-2, alpha 2 subunit mm
MU11KSUBB:X14972_S_AT adaptor protein complex AP-2, alpha 2 subunit mm
MU11KSUBB:MSA.11835.0_S_AT adaptor protein complex AP-2, alpha 2 subunit mm
MG-U74AV2:100492_AT adaptor protein complex AP-2, alpha 2 subunit mm
MG-U74AV2:102835_AT adaptor protein complex AP-2, alpha 2 subunit mm
MG-U74CV2:141027_AT adaptor protein complex AP-2, alpha 2 subunit mm
MOE430A:1435869_S_AT adaptor protein complex AP-2, alpha 2 subunit mm
MOUSE430A_2:1435869_S_AT adaptor protein complex AP-2, alpha 2 subunit mm
MOUSE430_2:1435869_S_AT adaptor protein complex AP-2, alpha 2 subunit mm
MOE430A:1455482_AT adaptor protein complex AP-2, alpha 2 subunit mm
MOUSE430A_2:1455482_AT adaptor protein complex AP-2, alpha 2 subunit mm
MOUSE430_2:1455482_AT adaptor protein complex AP-2, alpha 2 subunit mm
MG-U74CV2:168206_R_AT Adaptor protein complex AP-2, alpha 2 subunit, mRNA (cDNA clone IMAGE:3709070) mm
MU19KSUBA:TC14342_AT Adaptor protein complex AP-2, alpha 2 subunit, mRNA (cDNA clone IMAGE:3709070) mm
MU19KSUBA:TC23944_AT Adaptor protein complex AP-2, alpha 2 subunit, mRNA (cDNA clone IMAGE:3709070) mm
MU19KSUBA:TC23944_G_AT Adaptor protein complex AP-2, alpha 2 subunit, mRNA (cDNA clone IMAGE:3709070) mm
MU19KSUBC:TC40888_AT Adaptor protein complex AP-2, alpha 2 subunit, mRNA (cDNA clone IMAGE:3709070) mm
RG-U34A:X53773_AT adaptor protein complex AP-2, alpha 2 subunit rn
RAE230A:1369982_AT adaptor protein complex AP-2, alpha 2 subunit rn
RAT230_2:1369982_AT adaptor protein complex AP-2, alpha 2 subunit rn
RG-U34A:RC_AA800186_AT Adaptor protein complex AP-2, alpha 2 subunit rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6461 protein complex assembly inferred from electronic annotation QuickGO AmiGO
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5905 coated pit inferred from electronic annotation QuickGO AmiGO
30130 clathrin coat of trans-Golgi network vesicle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA68332 Adaptin, alpha chain (clathrin associated complex) protein 2 [Caenorhabditis elegans] ref|NP_509572.1| T20B5.1 [Caenorhabditis elegans] 0.0
blast CAE69834 Hypothetical protein CBG16158 [Caenorhabditis briggsae] 0.0
blast EAA05923 ENSANGP00000019991 [Anopheles gambiae str. PEST] ref|XP_310153.2| ENSANGP00000019991 [Anopheles gambiae str. PEST] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003164 EMBL-EBI Alpha adaptin, C-terminal 3.5E-108
Pfam IPR002553 EMBL-EBI Adaptin, N-terminal 1.0E-126
Pfam IPR008152 EMBL-EBI Alpha/gamma adaptin, C-terminal 2.6E-45
Pfam IPR003164 EMBL-EBI Alpha adaptin, C-terminal 3.5E-108
Pfam IPR002553 EMBL-EBI Adaptin, N-terminal 1.0E-126
Pfam IPR008152 EMBL-EBI Alpha/gamma adaptin, C-terminal 2.6E-45
Sequence
Target Sequence 
>C. ELEGANS:189016_AT
taataagttcaccccaatcatcacgtgccctggagcactagcagttcaactacaagctca
ggcaaaaccagtggagccggtggttgcagctggaactcaagttcagcaattgattaattt
tgtctgtgttcaagagttccagaagatgccaattatgaacataaagtttacattcacaga
tcgtgcgggtgctgttcaaaactttgataaaaacttctaccttccactcttcatcagcaa
attcttcgagcccacaaacatgacatccgagcaatttttcactagatggaagtcacttgg
tgcagcgtcacaggaagcacagaagattttcaacgcactgtcaccgattgagcacgcaac
tattgaatcaagactgaaaggatttggagcaaacttactgactgacgtggatccaaatcc
cgacaattatgtgtgcgctggaatcattcatacccagacgcaacagattggaactcttat
tcgtttggagccaaacaagcaagcaaagatgtaccgccttacaatccgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAATAAGTTCACCCCAATCATCACG 130 635 2151 Antisense
TCATCACGTGCCCTGGAGCACTAGC 273 619 2168 Antisense
GATCGTGCGGGTGCTGTTCAAAACT 545 417 2329 Antisense
TGATAAAAACTTCTACCTTCCACTC 680 515 2355 Antisense
CTTCCACTCTTCATCAGCAAATTCT 500 219 2371 Antisense
AATTCTTCGAGCCCACAAACATGAC 678 175 2390 Antisense
GGAAGTCACTTGGTGCAGCGTCACA 501 531 2438 Antisense
GAAGATTTTCAACGCACTGTCACCG 530 337 2472 Antisense
GACTGACGTGGATCCAAATCCCGAC 32 375 2550 Antisense
TCATTCATACCCAGACGCAACAGAT 333 619 2594 Antisense
AAGATGTACCGCCTTACAATCCGTT 283 153 2656 Antisense
Sequence Source Affymetrix Proprietary Database