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[189003_s_at] phosphorylase

Description K02D7.1 /REP_DB=WormBase Gene ID /WP=CE17994 /TR=O61217 /GB=AAC02605.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=phosphorylase
Cluster 6grp_highest_10_10 > 55      6grp_high1_10_10 > 55      4grp_highest_8_8 > 46      4grp_high_8_8 > 11      6grp_highest_nonorm > 37      6grp_total > 45      4grp_total > 13      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8361
1 2 3 4 range
A 8642.2
(0.3)
P 		5202.1
(-2.3)
P 		4203
(-3)
P 		12310.4
(3)
P 		8107
B 9757.6
(1.9)
P 		5118.1
(-2.2)
P 		4168.7
(-3)
P 		10985.6
(3)
P 		6817
C 9065.4
(0.9)
P 		4889.4
(-2.3)
P 		3949.8
(-3)
P 		11768.9
(3)
P 		7819
avr 9155.1
(1) 		5069.9
(-2.2) 		4107.2
(-3) 		11688.3
(3) 		7581

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1589
1 2 3 4 5 6 range
A 5497.4
(3)
P 		5381.6
(2.4)
P 		5114.9
(1)
P 		5190.9
(1.4)
P 		4335.6
(-3)
P 		5418.5
(2.6)
P 		1162
B 5052.5
(0.7)
P 		4612.2
(-0.9)
P 		5648.9
(3)
P 		4641.1
(-0.8)
P 		4059.5
(-3)
P 		4610.5
(-0.9)
P 		1589
C 4840.1
(0.2)
P 		5073.3
(1.2)
P 		5478.1
(3)
P 		5142.2
(1.5)
P 		4091.5
(-3)
P 		5248.5
(2)
P 		1387
avr 5130
(1.6) 		5022.4
(1.1) 		5414
(3) 		4991.4
(1) 		4162.2
(-3) 		5092.5
(1.5) 		1252
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2181
1 2 3 4 range
A 3146.6
(3)
P 		1129.2
(-2.9)
P 		1098.7
(-3)
P 		1802
(-0.9)
P 		2048
B 3111.6
(3)
P 		1264.1
(-2.2)
P 		966
(-3)
P 		2341
(0.8)
P 		2146
C 3000.9
(3)
P 		1405.7
(-2.9)
P 		1376.6
(-3)
P 		1984.4
(-0.8)
P 		1624
avr 3086.4
(3) 		1266.3
(-2.6) 		1147.1
(-3) 		2042.5
(-0.2) 		1939

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 901
1 2 3 4 5 6 range
A 1013.4
(-3)
P 		1914
(3)
P 		1113.9
(-2.3)
P 		1316.5
(-1)
P 		1308.3
(-1)
P 		1319.2
(-1)
P 		901
B 1758
(3)
P 		1535.2
(0.5)
P 		1371.7
(-1.4)
P 		1229.3
(-3)
P 		1649.3
(1.8)
P 		1719.2
(2.6)
P 		529
C 1794
(3)
P 		1548
(1.1)
P 		1394.3
(-0.2)
P 		1034.1
(-3)
P 		1323.9
(-0.7)
P 		1757
(2.7)
P 		760
avr 1521.8
(1.2) 		1665.7
(3) 		1293.3
(-1.7) 		1193.3
(-3) 		1427.2
(-0) 		1598.5
(2.1) 		472
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189003_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10766
Sequence Type Exemplar sequence
Representative Public ID K02D7.1 NCBI
Target Description K02D7.1 /REP_DB=WormBase Gene ID /WP=CE17994 /TR=O61217 /GB=AAC02605.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=phosphorylase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067499(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067499 NCBI Caenorhabditis elegans K02D7.1 (phosphorylase) mRNA, complete cds. 11/11 None
SNAP00000015226 ENSEMBL cdna:SNAP chromosome:CEL140:IV:292646:294155:-1 11/11 None
GENEFINDER00000015238 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:292646:298535:-1 11/11 None
K02D7.1 ENSEMBL cdna:known chromosome:CEL140:IV:292438:294676:-1 gene:K02D7.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:292645-294645(-) 100.0 100.0
Public Domain and Genome References
Gene Title phosphorylase
Ensembl K02D7.1
Entrez Gene 176851 Entrez gene
SwissProt O61217 EMBL-EBI
WormBase CE17994 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1583154_AT similar to Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP) cfa
CANINE_2:CFA.3232.1.A1_AT similar to Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP) cfa
CANINE_2:CFAAFFX.9062.1.S1_S_AT similar to Purine nucleoside phosphorylase (Inosine phosphorylase) (PNP) cfa
DROSOPHILA_2:1626799_A_AT dm
DROSGENOME1:142161_S_AT dm
CHICKEN:GGAAFFX.1566.1.S1_AT similar to Hypothetical protein zgc:77182 gga
HG-FOCUS:201695_S_AT nucleoside phosphorylase hs
HG-U133A:201695_S_AT nucleoside phosphorylase hs
HG-U133_PLUS_2:201695_S_AT nucleoside phosphorylase hs
HG-U133A_2:201695_S_AT nucleoside phosphorylase hs
HG-U95AV2:430_AT nucleoside phosphorylase hs
HC-G110:430_AT nucleoside phosphorylase hs
HU35KSUBD:RC_H72853_S_AT nucleoside phosphorylase hs
HUGENEFL:K02574_AT nucleoside phosphorylase hs
U133_X3P:G4557800_3P_A_AT nucleoside phosphorylase hs
RG-U34C:RC_AI102495_AT nucleoside phosphorylase rn
RAE230A:1388337_AT nucleoside phosphorylase rn
RAT230_2:1388337_AT nucleoside phosphorylase rn
RAE230B:1396245_AT nucleoside phosphorylase rn
RAT230_2:1396245_AT nucleoside phosphorylase rn
YEAST_2:1775347_AT Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
4645 phosphorylase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC02605 Hypothetical protein K02D7.1 [Caenorhabditis elegans] ref|NP_499900.1| K02D7.1 [Caenorhabditis elegans] 1.0E-171
blast CAE67904 Hypothetical protein CBG13501 [Caenorhabditis briggsae] 1.0E-160
blast AAC02605 Hypothetical protein K02D7.1 [Caenorhabditis elegans] ref|NP_499900.1| K02D7.1 [Caenorhabditis elegans] 1.0E-160
blast CAE67904 Hypothetical protein CBG13501 [Caenorhabditis briggsae] 1.0E-150
blast CAE70406 Hypothetical protein CBG16977 [Caenorhabditis briggsae] 3.0E-66
Protein Domains
Database ID Description E-Value
Pfam IPR001369 EMBL-EBI Purine (and other) phosphorylase, family 2 2.0E-77
Pfam IPR001369 EMBL-EBI Purine (and other) phosphorylase, family 2 2.0E-77
Pfam IPR001369 EMBL-EBI Purine (and other) phosphorylase, family 2 2.0E-77
Sequence
Target Sequence 
>C. ELEGANS:189003_S_AT
atatggatttggctttgtgcaccctcccagtccgtgtaatgcaccagcttggtatcaaga
tcatgattgtctccaacgcagcgggaggtatcaatgccgtgttgagacacggagacttga
tgctcatcaaggatcatattttccttccggcccttgccggattctctccacttgtcggat
gcaacgatcccagatttggagcccgatttgtatcggttcatgatgcctatgataagcagc
ttagacaactcgccattgatgttggccgtcgtagtgatatgacgttgtacgagggtgtct
atgtgatgtccggtggaccacaatacgagtcaccagctgaggtatcactcttcaaaacag
tcggagccgacgcactcggaatgtcaacttgccacgaggttaccgtagctcgtcagtgtg
gaattaaggtccttggtttctctcttattaccaatattgcaaacttggatgcagatgcat
cagtcgaagtgtcacatgaagaagttatggatatcgcgcaacaagccggtgaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATATGGATTTGGCTTTGTGCACCCT 543 21 338 Antisense
CCAGTCCGTGTAATGCACCAGCTTG 336 271 364 Antisense
AGATCATGATTGTCTCCAACGCAGC 296 67 395 Antisense
AACGATCCCAGATTTGGAGCCCGAT 298 145 520 Antisense
TAAGCAGCTTAGACAACTCGCCATT 642 635 570 Antisense
GAGGGTGTCTATGTGATGTCCGGTG 567 401 628 Antisense
GTCACCAGCTGAGGTATCACTCTTC 583 465 666 Antisense
GTCAACTTGCCACGAGGTTACCGTA 535 299 720 Antisense
GTTACCGTAGCTCGTCAGTGTGGAA 594 445 736 Antisense
TTAAGGTCCTTGGTTTCTCTCTTAT 395 685 761 Antisense
GATATCGCGCAACAAGCCGGTGAAC 241 425 847 Antisense
Sequence Source Affymetrix Proprietary Database