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[189002_s_at]

Description F53G12.5 /REP_DB=WormBase Gene ID /WP=CE25000 /CHR=1 /FEA=Sanger Annotation /DEF=locus:mex-3 (ST.LOUIS) TR:O01797 protein_id:AAB54161.1
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 77      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_highest_nonorm > 94      6grp_total > 78      4grp_total > 14      6grp_highest_sub > Da      6grp_cgc > 49      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 169
1 2 3 4 range
A 371.8
(-3)
P 		377
(-2.2)
P 		412.7
(3)
P 		406.5
(2.1)
P 		41
B 424.8
(2)
P 		313.8
(-1.9)
P 		284.1
(-3)
P 		453.4
(3)
P 		169
C 397.8
(2.8)
P 		371.2
(-3)
P 		373.1
(-2.6)
P 		398.7
(3)
P 		28
avr 398.1
(1) 		354
(-3) 		356.6
(-2.8) 		419.5
(3) 		66

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1321
1 2 3 4 5 6 range
A 331.2
(-2.6)
P 		245.7
(-3)
P 		265
(-2.9)
P 		273.2
(-2.9)
P 		680.8
(-1)
P 		1542.3
(3)
P 		1297
B 350
(-2.4)
P 		267.5
(-2.8)
P 		221.1
(-3)
P 		248.7
(-2.9)
P 		696.1
(-0.8)
P 		1527.7
(3)
P 		1307
C 303
(-2.6)
P 		241.7
(-2.9)
P 		224.4
(-3)
P 		357.2
(-2.4)
P 		616.2
(-1.1)
P 		1464.3
(3)
P 		1240
avr 328.1
(-2.6) 		251.6
(-2.9) 		236.8
(-3) 		293
(-2.7) 		664.4
(-1) 		1511.4
(3) 		1275
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 135.4
(3)
P 		81.8
(-1.2)
P 		107.9
(0.8)
P 		59.5
(-3)
P 		76
B 135.5
(3)
P 		77.5
(-2)
P 		65.8
(-3)
P 		96.6
(-0.3)
P 		70
C 131.7
(3)
P 		106.7
(0.7)
P 		130
(2.8)
P 		67.2
(-3)
P 		65
avr 134.2
(3) 		88.7
(-1.6) 		101.2
(-0.3) 		74.4
(-3) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 516
1 2 3 4 5 6 range
A 61
(-2.9)
P 		87.4
(-2.4)
P 		57.7
(-3)
P 		69.3
(-2.8)
P 		205.4
(-0.2)
P 		375.5
(3)
P 		318
B 121.8
(-2.2)
P 		89.1
(-2.6)
P 		53.7
(-3)
P 		65.9
(-2.9)
P 		282.8
(-0.3)
P 		569.7
(3)
P 		516
C 112.3
(-2.2)
P 		73.8
(-2.8)
P 		57.1
(-3)
P 		71.8
(-2.8)
P 		199.4
(-1)
P 		490.2
(3)
P 		433
avr 98.4
(-2.4) 		83.4
(-2.6) 		56.2
(-3) 		69
(-2.8) 		229.2
(-0.5) 		478.5
(3) 		422
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189002_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2587
Sequence Type Exemplar sequence
Representative Public ID F53G12.5 NCBI
Target Description F53G12.5 /REP_DB=WormBase Gene ID /WP=CE25000 /CHR=1 /FEA=Sanger Annotation /DEF=locus:mex-3 (ST.LOUIS) TR:O01797 protein_id:AAB54161.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058280(11), NM_001026318(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058280 NCBI Caenorhabditis elegans Muscle EXcess family member (mex-3) (mex-3) mRNA, complete cds. 11/11 A 
NM_001026318 NCBI Caenorhabditis elegans Muscle EXcess family member (mex-3) (mex-3) mRNA, complete cds. 11/11 None
GENEFINDER00000020936 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:127297:133582:1 11/11 None
SNAP00000020925 ENSEMBL cdna:SNAP chromosome:CEL140:I:131471:133582:1 11/11 None
F53G12.5b ENSEMBL cdna:known chromosome:CEL140:I:127297:134242:1 gene:F53G12.5 11/11 A 
F53G12.5a.2 ENSEMBL cdna:known chromosome:CEL140:I:128695:133809:1 gene:F53G12.5 11/11 None
F53G12.5a.1 ENSEMBL cdna:known chromosome:CEL140:I:128697:133582:1 gene:F53G12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:128697-133582(+) 100.0 100.0
Public Domain and Genome References
Ensembl F53G12.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK73873 Muscle excess protein 3, isoform b [Caenorhabditis elegans] ref|NP_490681.2| Muscle EXcess family member (mex-3) [Caenorhabditis elegans] 0.0
blast AAK73872 Muscle excess protein 3, isoform a [Caenorhabditis elegans] ref|NP_001021489.1| Muscle EXcess family member (mex-3) [Caenorhabditis elegans] 0.0
blast AAC47313 MEX-3 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004087 EMBL-EBI KH 5.9E-6
Pfam IPR004087 EMBL-EBI KH 5.9E-6
Sequence
Target Sequence 
>C. ELEGANS:189002_S_AT
ttgccggacagttggccggtgtctcgttgatggtgcagaagcagcaacaggctcaacaac
aaatgcaggaggctcagcaacaatcgatgttctatcgaagagcattcggcaacagtaatc
cgttcaatcagaaggagatgtcgtcgtcgccattcggaatggagagctcgttgggattgg
acgcgttgctccgcagtttcccatcgatgcgtagttcgttgactccggaatctctttccg
gtactggactgtcttctcgtccatcgttgggaggaggacaatcggcgaaacaagatctgc
caacctacgactactggggaaccaacaactcgcttaatgatattatggagaacgaaattc
tcagccgcaagtacgatgccctgtccgcatggtcgtcgatgggattggagaaacgcgagg
aatccccaaccaatgggctcatgtcctccctcaaaggaacatccgccggcttcggtcttc
tcagcacaatctggtctggcggaaacatgaatctgagcccaggatcgttggcttcagcat
ctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGCCGGACAGTTGGCCGGTGTCTC 472 695 656 Antisense
GCAGTTTCCCATCGATGCGTAGTTC 144 313 848 Antisense
GTAGTTCGTTGACTCCGGAATCTCT 578 451 866 Antisense
TCTTCTCGTCCATCGTTGGGAGGAG 664 615 907 Antisense
TCTGCCAACCTACGACTACTGGGGA 307 599 951 Antisense
ATTCTCAGCCGCAAGTACGATGCCC 189 11 1012 Antisense
TGTCCGCATGGTCGTCGATGGGATT 77 557 1037 Antisense
GAGGAATCCCCAACCAATGGGCTCA 278 405 1072 Antisense
TGTCCTCCCTCAAAGGAACATCCGC 443 557 1097 Antisense
TTCTCAGCACAATCTGGTCTGGCGG 338 689 1133 Antisense
CCAGGATCGTTGGCTTCAGCATCTG 275 263 1174 Antisense
Sequence Source Affymetrix Proprietary Database