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[188988_at]

Description F36A2.9 /REP_DB=WormBase Gene ID /WP=CE17779 /TR=P90862 /GB=CAB03072.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=phospholipase
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 77      4grp_highest_8_8 > 45      4grp_high_8_8 > 08      6grp_highest_nonorm > 73      6grp_total > 78      4grp_total > 15      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 288
1 2 3 4 range
A 563.3
(3)
P 		349.3
(-3)
P 		436.1
(-0.6)
P 		541.4
(2.4)
P 		214
B 631.8
(3)
P 		402.7
(-3)
P 		413.1
(-2.7)
P 		500.5
(-0.4)
P 		229
C 573.8
(3)
P 		343.9
(-3)
P 		485.4
(0.7)
P 		575.4
(3)
P 		232
avr 589.6
(3) 		365.3
(-3) 		444.9
(-0.9) 		539.1
(1.6) 		224

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 373
1 2 3 4 5 6 range
A 491.4
(-2.9)
P 		499
(-2.6)
P 		488.7
(-3)
P 		516.5
(-2)
P 		642.8
(2.7)
P 		650.3
(3)
P 		162
B 484.2
(-2.2)
P 		531.3
(-0.5)
P 		460.5
(-3)
P 		528.3
(-0.6)
P 		535.1
(-0.4)
P 		632.5
(3)
P 		172
C 413.3
(-3)
P 		532.7
(-1.1)
P 		435.9
(-2.6)
P 		420.8
(-2.9)
P 		453.2
(-2.4)
P 		786.4
(3)
P 		373
avr 463
(-3) 		521
(-1.4) 		461.7
(-3) 		488.5
(-2.3) 		543.7
(-0.8) 		689.7
(3) 		228
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 129
1 2 3 4 range
A 205.1
(3)
P 		75.8
(-3)
P 		114
(-1.2)
P 		79.3
(-2.8)
P 		129
B 201.5
(3)
P 		99.5
(-2.8)
P 		95.7
(-3)
P 		106.7
(-2.4)
P 		106
C 189.9
(3)
P 		98.9
(-2.9)
P 		169.2
(1.7)
P 		97
(-3)
P 		93
avr 198.8
(3) 		91.4
(-3) 		126.3
(-1.1) 		94.3
(-2.8) 		107

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 179
1 2 3 4 5 6 range
A 90.6
(-3)
P 		177.5
(2)
P 		106.4
(-2.1)
P 		131
(-0.7)
P 		194
(3)
P 		158.3
(0.9)
P 		103
B 168.5
(-0.3)
P 		176.8
(0.1)
P 		111.8
(-3)
P 		139.9
(-1.6)
P 		217.4
(2.1)
P 		235.8
(3)
P 		124
C 153.2
(-0.7)
P 		162.6
(-0.4)
P 		110.9
(-2.1)
P 		84.6
(-3)
P 		146.6
(-0.9)
P 		263.2
(3)
P 		179
avr 137.4
(-1.5) 		172.3
(0.4) 		109.7
(-3) 		118.5
(-2.5) 		186
(1.2) 		219.1
(3) 		109
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188988_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2394
Sequence Type Exemplar sequence
Representative Public ID F36A2.9 NCBI
Target Description F36A2.9 /REP_DB=WormBase Gene ID /WP=CE17779 /TR=P90862 /GB=CAB03072.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=phospholipase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059984(11), NM_182068(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059984 NCBI Caenorhabditis elegans F36A2.9a (phospholipase) mRNA, complete cds. 11/11 None
NM_182068 NCBI Caenorhabditis elegans F36A2.9b (phospholipase) mRNA, complete cds. 11/11 None
SNAP00000004925 ENSEMBL cdna:SNAP chromosome:CEL140:I:8825106:8829103:1 11/11 None
GENEFINDER00000004945 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8825106:8827560:1 11/11 None
F36A2.9b ENSEMBL cdna:known chromosome:CEL140:I:8825106:8829463:1 gene:F36A2.9 11/11 A 
F36A2.9a ENSEMBL cdna:known chromosome:CEL140:I:8825106:8829464:1 gene:F36A2.9 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8825036-8827491(+) 100.0 100.0
Public Domain and Genome References
Ensembl F36A2.9
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03072 Hypothetical protein F36A2.9a [Caenorhabditis elegans] ref|NP_492385.2| F36A2.9a [Caenorhabditis elegans] 0.0
blast CAD56582 Hypothetical protein F36A2.9b [Caenorhabditis elegans] ref|NP_871868.1| F36A2.9b [Caenorhabditis elegans] 0.0
blast CAE66981 Hypothetical protein CBG12377 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001087 EMBL-EBI Lipolytic enzyme, G-D-S-L 3.1E-10
Pfam IPR001087 EMBL-EBI Lipolytic enzyme, G-D-S-L 2.5E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_871868.1 1 468-490
F36A2.9b 1 468-490
SNAP00000004925 1 468-490
Sequence
Target Sequence 
>C. ELEGANS:188988_AT
gaatcatcagcttaacgtggcggttaccggagcaacgaccgaagatcttccagatcaagc
aagacgattaacacgcagactgaaaaaattatcagagctagattatcataatgaatggat
tatgataattattactattgggacagaagaactttgctctcgatgtgaaggaccttcgta
tgataacattagaaaagctattgaacatctgcaaatagaaattccaaaggctttggttgt
tcttttgggacctgttcatgtatcatccttccacgagcagaaatcaaatcttttgaaagc
ccgttgtgcatgttctcgagaccaaaccgaagggttcatgtatgatgtgtctcgaaaatg
gtccaaagtttggcgagatgttcaaaaatttgtggagaatggagttacagccagaccaac
attcggaatgatcagttatccaatggtaactataacctcaagatatccaagtggattgtt
cattcgagataagccattattgaatcgaagaggtcataattacgcgacgaagtggttatg
gaataggctgattggcggagatttatataatctttcctcggctacact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCATCAGCTTAACGTGGCGGTT 446 327 663 Antisense
TGGCGGTTACCGGAGCAACGACCGA 309 543 680 Antisense
GCAACGACCGAAGATCTTCCAGATC 321 317 694 Antisense
GGGACAGAAGAACTTTGCTCTCGAT 269 495 802 Antisense
TTTGCTCTCGATGTGAAGGACCTTC 618 667 815 Antisense
GTTGTTCTTTTGGGACCTGTTCATG 403 437 898 Antisense
TGTATCATCCTTCCACGAGCAGAAA 341 561 921 Antisense
TTGAAAGCCCGTTGTGCATGTTCTC 501 703 955 Antisense
TGTGCATGTTCTCGAGACCAAACCG 226 557 967 Antisense
GAGTTACAGCCAGACCAACATTCGG 530 397 1064 Antisense
TATATAATCTTTCCTCGGCTACACT 510 661 1226 Antisense
Sequence Source Affymetrix Proprietary Database