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[188985_at] hyaluronidase

Description T22C8.2 /REP_DB=WormBase Gene ID /WP=CE02350 /TR=Q22675 /GB=CAA88874.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=hyaluronidase
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 91      4grp_high_8_8 > 33      6grp_highest_nonorm > 91      6grp_total > 94      4grp_total > 46      6grp_high1_sub > 05      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 249
1 2 3 4 range
A 319.4
(3)
P 		152.6
(-2.3)
P 		132.2
(-3)
P 		165.5
(-1.9)
A 		187
B 269.8
(3)
P 		152.8
(-1.5)
P 		112.3
(-3)
A 		130.2
(-2.3)
P 		158
C 361.3
(3)
P 		180.7
(-1.8)
P 		136.5
(-3)
P 		172.4
(-2)
M 		225
avr 316.8
(3) 		162
(-1.9) 		127
(-3) 		156
(-2.1) 		190

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 250
1 2 3 4 5 6 range
A 203.7
(-1.9)
P 		177.6
(-2.6)
P 		161.4
(-3)
P 		278.5
(0)
P 		394
(3)
P 		253.4
(-0.6)
P 		233
B 297.3
(1.7)
P 		206.7
(-1.4)
P 		160.6
(-3)
P 		189.9
(-2)
P 		333.8
(3)
P 		209.1
(-1.3)
P 		173
C 213.2
(-1.1)
P 		195.9
(-1.6)
P 		143.8
(-3)
P 		363.3
(3)
P 		316.8
(1.7)
P 		232.3
(-0.6)
P 		220
avr 238.1
(-0.4) 		193.4
(-1.8) 		155.3
(-3) 		277.2
(0.8) 		348.2
(3) 		231.6
(-0.6) 		193
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 116.3
(3)
P 		33.1
(-2.4)
P 		34.5
(-2.3)
P 		24.2
(-3)
A 		92
B 86.1
(3)
P 		37.7
(-1.8)
P 		26
(-3)
A 		27.7
(-2.8)
P 		60
C 119.6
(3)
P 		51.9
(-1.5)
P 		47.6
(-1.8)
P 		29.1
(-3)
M 		91
avr 107.3
(3) 		40.9
(-2) 		36
(-2.3) 		27
(-3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 37.5
(-2.8)
P 		63.2
(-1)
P 		35.1
(-3)
P 		70.6
(-0.5)
P 		118.9
(3)
P 		61.7
(-1.1)
P 		84
B 103.4
(1)
P 		68.8
(-1.1)
P 		39
(-3)
P 		50.3
(-2.3)
P 		135.6
(3)
P 		78
(-0.6)
P 		97
C 79
(0.9)
P 		59.8
(-0.9)
P 		36.6
(-3)
P 		73.1
(0.3)
P 		102.5
(3)
P 		77.8
(0.8)
P 		66
avr 73.3
(-0.3) 		63.9
(-1) 		36.9
(-3) 		64.7
(-1) 		119
(3) 		72.5
(-0.4) 		82
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188985_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3782
Sequence Type Exemplar sequence
Representative Public ID T22C8.2 NCBI
Target Description T22C8.2 /REP_DB=WormBase Gene ID /WP=CE02350 /TR=Q22675 /GB=CAA88874.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=hyaluronidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063429(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063429 NCBI Caenorhabditis elegans T22C8.2 (hyaluronidase) mRNA, complete cds. 11/11 None
SNAP00000014908 ENSEMBL cdna:SNAP chromosome:CEL140:II:8614552:8617147:-1 11/11 None
GENEFINDER00000014915 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8614552:8617147:-1 11/11 None
T22C8.2 ENSEMBL cdna:known chromosome:CEL140:II:8614468:8617525:-1 gene:T22C8.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8614548-8617144(-) 100.0 100.0
Public Domain and Genome References
Gene Title hyaluronidase
Ensembl T22C8.2
Entrez Gene 174383 Entrez gene
SwissProt Q22675 EMBL-EBI
WormBase CE02350 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1599595_AT similar to hyaluronoglucosaminidase 1 isoform 1 cfa
CANINE_2:CFA.14812.1.A1_AT similar to hyaluronoglucosaminidase 1 isoform 1 cfa
CANINE_2:CFAAFFX.16568.1.S1_S_AT similar to hyaluronoglucosaminidase 1 isoform 1 cfa
CANINE_2:CFAAFFX.16579.1.S1_AT similar to hyaluronoglucosaminidase 1 isoform 1 cfa
CHICKEN:GGAAFFX.517.1.S1_AT similar to Hyaluronidase 1 gga
CHICKEN:GGAAFFX.517.2.S1_S_AT similar to Hyaluronidase 1 gga
HG-U95AV2:1546_AT hyaluronoglucosaminidase 1 hs
HC-G110:1546_AT hyaluronoglucosaminidase 1 hs
HG-U133_PLUS_2:210619_S_AT hyaluronoglucosaminidase 1 hs
HG-U133A:210619_S_AT hyaluronoglucosaminidase 1 hs
HG-FOCUS:210619_S_AT hyaluronoglucosaminidase 1 hs
HG-U133A_2:210619_S_AT hyaluronoglucosaminidase 1 hs
HUGENEFL:U03056_AT hyaluronoglucosaminidase 1 hs
U133_X3P:G5825510_3P_A_AT hyaluronoglucosaminidase 1 hs
HG-U95AV2:37176_AT hyaluronoglucosaminidase 1 hs
MOUSE430A_2:1449954_AT hyaluronidase 1 mm
MOUSE430_2:1449954_AT hyaluronidase 1 mm
MOE430A:1449954_AT hyaluronidase 1 mm
MG-U74AV2:102230_AT hyaluronidase 1 mm
MG-U74BV2:109370_AT hyaluronidase 1 mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
6952 defense response inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4415 hyalurononglucosaminidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA88874 Hypothetical protein T22C8.2 [Caenorhabditis elegans] ref|NP_495830.1| T22C8.2 [Caenorhabditis elegans] 0.0
blast CAE57557 Hypothetical protein CBG00535 [Caenorhabditis briggsae] 0.0
blast XP_424356 PREDICTED: similar to Hyaluronidase 1 [Gallus gallus] 5.0E-52
Protein Domains
Database ID Description E-Value
Pfam IPR001968 EMBL-EBI Glycoside hydrolase, family 56 1.0E-126
Pfam IPR001968 EMBL-EBI Glycoside hydrolase, family 56 6.5E-22
Pfam IPR001968 EMBL-EBI Glycoside hydrolase, family 56 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:188985_AT
ttgccagcacattggatcttgccataaaaatgaacgcgaacagtgttgtgatttggagta
catctcaatcgatcggaagtcgttgtggatcacttcagacttatgtagataacacattgg
gtccgtatcttcaattaacagaccgaaatttggacaaatgtcgcatggagagatgtgaag
ggcgtggggaatgctatttaccacgaccgaaaacaaatccagctatctataactttgcct
gccgctgtgaacgtccatatttcggaaaaagctgtgaataccgtggccgtcgaatgggtg
tatcaatgccaaaagcttctcaaactcctcaagtcattccagatgtcactgcctactt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGCCAGCACATTGGATCTTGCCAT 239 701 938 Antisense
GTTGTGGATCACTTCAGACTTATGT 394 437 1019 Antisense
ATAACACATTGGGTCCGTATCTTCA 363 25 1046 Antisense
GAATGCTATTTACCACGACCGAAAA 192 329 1126 Antisense
AGCTATCTATAACTTTGCCTGCCGC 454 77 1158 Antisense
GCCGCTGTGAACGTCCATATTTCGG 558 285 1178 Antisense
AAAAGCTGTGAATACCGTGGCCGTC 411 131 1204 Antisense
CCGTGGCCGTCGAATGGGTGTATCA 541 265 1218 Antisense
ATGCCAAAAGCTTCTCAAACTCCTC 707 37 1243 Antisense
CTCAAACTCCTCAAGTCATTCCAGA 640 227 1256 Antisense
TCATTCCAGATGTCACTGCCTACTT 361 619 1271 Antisense
Sequence Source Affymetrix Proprietary Database