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[188963_at] histone H4

Description T23D8.5 /REP_DB=WormBase Gene ID /WP=CE03252 /TR=SW:P02306 /GB=CAB03396.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=histone H4
Cluster 6grp_total > 95      4grp_total > 40      6grp_cgc > 78      6grp_cgc_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 65
1 2 3 4 range
A 35.7
(-0.5)
A 		42.5
(0.3)
A 		66.1
(3)
A 		14.4
(-3)
A 		52
B 40.5
(0.3)
A 		13.3
(-3)
A 		62.2
(3)
A 		21.4
(-2)
A 		49
C 6.4
(-3)
A 		15.4
(-2.2)
A 		10.8
(-2.6)
A 		71.8
(3)
P 		65
avr 27.5
(-2) 		23.7
(-3) 		46.4
(3) 		35.9
(0.2) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 76
1 2 3 4 5 6 range
A 7
(-3)
A 		21.1
(-1.9)
A 		15
(-2.4)
A 		67.8
(1.8)
A 		83.2
(3)
A 		61.3
(1.3)
A 		76
B 29.8
(-1.1)
A 		52.7
(0.8)
A 		7.6
(-3)
A 		73.4
(2.5)
A 		79.3
(3)
A 		63.5
(1.7)
A 		72
C 36.8
(-2.9)
A 		66
(2)
A 		69.2
(2.5)
A 		36.5
(-3)
A 		57.7
(0.6)
A 		72.2
(3)
A 		36
avr 24.5
(-3) 		46.6
(-0.3) 		30.6
(-2.3) 		59.2
(1.3) 		73.4
(3) 		65.7
(2.1) 		49
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 13
(1.3)
A 		9.2
(-0.2)
A 		17.3
(3)
A 		2.1
(-3)
A 		15
B 12.9
(2.2)
A 		3.3
(-3)
A 		14.4
(3)
A 		4.6
(-2.3)
A 		11
C 2.1
(-3)
A 		4.4
(-1.6)
A 		3.8
(-2)
A 		12.1
(3)
P 		10
avr 9.3
(0.6) 		5.6
(-3) 		11.8
(3) 		6.3
(-2.3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 1.3
(-3)
A 		7.5
(-1.4)
A 		3.3
(-2.5)
A 		17.2
(1)
A 		25.1
(3)
A 		14.9
(0.4)
A 		24
B 10.4
(-1.3)
A 		17.5
(0.1)
A 		1.8
(-3)
A 		19.4
(0.5)
A 		32.2
(3)
A 		23.7
(1.3)
A 		30
C 13.6
(-0.8)
A 		20.2
(1.6)
A 		17.6
(0.7)
A 		7.3
(-3)
A 		18.7
(1)
A 		24.2
(3)
A 		17
avr 8.4
(-2.7) 		15.1
(-0.5) 		7.6
(-3) 		14.6
(-0.6) 		25.3
(3) 		20.9
(1.5) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188963_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1592
Sequence Type Exemplar sequence
Representative Public ID T23D8.5 NCBI
Target Description T23D8.5 /REP_DB=WormBase Gene ID /WP=CE03252 /TR=SW:P02306 /GB=CAB03396.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=histone H4
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060240(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060240 NCBI Caenorhabditis elegans HIStone family member (his-67) (his-67) mRNA, complete cds. 11/11 None
SNAP00000036751 ENSEMBL cdna:SNAP chromosome:CEL140:I:9996573:9996884:-1 11/11 None
GENEFINDER00000036760 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9996573:9996884:-1 11/11 None
T23D8.5 ENSEMBL cdna:known chromosome:CEL140:I:9996573:9996884:-1 gene:T23D8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11338498-11338810(+) 90.06 90.06
chrV:16045102-16045414(+) 90.06 90.06
chrV:8900815-8901127(+) 90.06 90.06
chrI:9996503-9996815(-) 100.0 100.0
chrIV:11325994-11326306(-) 90.06 90.06
Public Domain and Genome References
Gene Title histone H4
Gene Symbol his-67
Ensembl T23D8.5
Entrez Gene 188792 Entrez gene
SwissProt P62784 EMBL-EBI
WormBase CE03252 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:15534_F_AT histone H4 at
ATH1-121501:247692_S_AT histone H4 at
ATH1-121501:266226_AT histone H4 at
CANINE_2:CFAAFFX.251.1.S1_S_AT similar to germinal histone H4 gene cfa
DROSOPHILA_2:1635715_AT dm
HG-U133_PLUS_2:208046_AT histone 1, H4a hs
HG-U133A:208046_AT histone 1, H4a hs
HG-FOCUS:208046_AT histone 1, H4a hs
HG-U133A_2:208046_AT histone 1, H4a hs
U133_X3P:G5579462_3P_AT histone 1, H4a hs
MU11KSUBB:MSA.753.0_AT histone 2, H4 mm
RG-U34A:RC_AA946439_AT similar to germinal histone H4 gene rn
RAE230A:1392476_AT similar to germinal histone H4 gene rn
RAT230_2:1392476_AT similar to germinal histone H4 gene rn
YEAST_2:1778426_S_AT One of two identical histone H4 proteins (see also HHF1); core histone required for chromatin assembly and chromosome function; contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6334 nucleosome assembly inferred from electronic annotation QuickGO AmiGO
7001 chromosome organization and biogenesis (sensu Eukaryota) inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
786 nucleosome inferred from electronic annotation QuickGO AmiGO
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05839 Hypothetical protein ZK131.8 [Caenorhabditis elegans] emb|CAB05837.1| Hypothetical protein ZK131.4 [Caenorhabditis elegans] emb|CAB05835.4| Hypothetical protein ZK131.1 [Caenorhabditis elegans] emb|CAB03396.1| Hypothetical protein T23D8.5 [Caenorhabditis elegans] emb|CAA94742.1| Hypothetical protein C50F4.7 [Caenorhabditis elegans] emb|CAA97407.1| Hypothetical protein B0035.9 [Caenorhabditis elegans] emb|CAA92734.1| Hypothetical protein F22B3.1 [Caenorhabditis elegans] emb|CAB07657.1| Hypothetical protein T10C6.14 [Caenorhabditis elegans] emb|CAB05210.1| Hypothetical protein F54E12.3 [Caenorhabditis elegans] gb|AAC05101.1| Histone protein 31 [Caenorhabditis elegans] gb|AAK84518.1| Histone protein 50 [Caenorhabditis elegans] gb|AAF98220.1| Histone protein 28 [Caenorhabditis elegans] gb|AAF98223.1| Histone protein 18 [Caenorhabditis elegans] gb|AAA83329.1| Histone protein 38 [Caenorhabditis elegans] emb|CAA24645.1| unnamed protein product [Strongylocentrotus purpuratus] emb|CAA38053.1| histone H4 [Pycnopodia helianthoides] emb|CAA38051.1| histone H4 [Pisaster ochraceus] emb|CAA38049.1| H4 histone [Pisaster brevispinus] emb|CAA33643.1| Histone protein [Caenorhabditis elegans] emb|CAA27581.1| unnamed protein product [Strongylocentrotus purpuratus] emb|CAA25241.1| unnamed protein product [Lytechinus pictus] gb|AAC48026.1| Histone protein 5 [Caenorhabditis elegans] ref|NP_509231.1| K03A1.6 [Caenorhabditis elegans] ref|NP_501406.1| HIStone family member (his-31) [Caenorhabditis elegans] ref|NP_496893.1| HIStone family member (his-10) [Caenorhabditis elegans] ref|NP_507034.1| HIStone family member (his-1) [Caenorhabditis elegans] ref|NP_492641.1| HIStone family member (his-67) [Caenorhabditis elegans] ref|NP_505466.1| HIStone family member (his-37) [Caenorhabditis elegans] ref|NP_505298.1| HIStone family member (his-18) [Caenorhabditis elegans] ref|NP_505291.1| HIStone family member (his-28) [Caenorhabditis elegans] ref|NP_505275.1| HIStone family member (his-50) [Caenorhabditis elegans] ref|NP_505200.1| HIStone family member (his-5) [Caenorhabditis elegans] ref|NP_502154.1| HIStone family member (his-64) [Caenorhabditis elegans] ref|NP_502139.1| HIStone family member (his-56) [Caenorhabditis elegans] ref|NP_502133.1| HIStone family member (his-46) [Caenorhabditis elegans] ref|NP_496896.1| HIStone family member (his-26) [Caenorhabditis elegans] ref|NP_496889.1| HIStone family member (his-14) [Caenorhabditis elegans] ref|XP_780884.1| PREDICTED: similar to histone (his-67) [Strongylocentrotus purpuratus] ref|XP_781796.1| PREDICTED: similar to histone (his-67) [Strongylocentrotus purpuratus] ref|NP_999707.1| H4 histone protein [Strongylocentrotus purpuratus] ref|NP_999716.1| late histone gene L1 H4 [Strongylocentrotus purpuratus] ref|NP_999715.1| late histone gene L2 H4 [Strongylocentrotus purpuratus] ref|NP_999713.1| late embryonic histone H4 [Strongylocentrotus purpuratus] sp|P62779|H4_PYCHE Histone H4 sp|P62778|H4_PISOC Histone H4 sp|P62777|H4_PISBR Histone H4 sp|P62780|H4_PARLI Histone H4 sp|P62782|H4_LYTPI Histone H4 sp|P62776|H4_HOLTU Histone H4 sp|P62784|H4_CAEEL Histone H4 emb|CAE60210.1| Hypothetical protein CBG03774 [Caenorhabditis briggsae] emb|CAE72198.1| Hypothetical protein CBG19306 [Caenorhabditis briggsae] emb|CAE62043.1| Hypothetical protein CBG06059 [Caenorhabditis briggsae] emb|CAE62040.1| Hypothetical protein CBG06056 [Caenorhabditis briggsae] emb|CAE61894.1| Hypothetical protein CBG05885 [Caenorhabditis briggsae] emb|CAE61864.1| Hypothetical protein CBG05842 [Caenorhabditis briggsae] emb|CAE61861.1| Hypothetical protein CBG05839 [Caenorhabditis briggsae] emb|CAE75444.1| Hypothetical protein CBG23438 [Caenorhabditis briggsae] emb|CAE58375.1| Hypothetical protein CBG01504 [Caenorhabditis briggsae] emb|CAE58373.1| Hypothetical protein CBG01500 [Caenorhabditis briggsae] gb|AAB48834.1| cleavage stage histone H4 [Psammechinus miliaris] pir||S01618 histone H4, embryonic (clones L1 and L2) - sea urchin (Strongylocentrotus purpuratus) emb|CAA86298.1| histone H4 [Holothuria tubulosa] emb|CAA29849.1| unnamed protein product [Strongylocentrotus purpuratus] emb|CAA29847.1| unnamed protein product [Strongylocentrotus purpuratus] emb|CAA76307.1| histone H4 [Paracentrotus lividus] emb|CAA25630.1| histone H4 (aa 1-103) [Psammechinus miliaris] sp|P62783|H4_STRPU Histone H4 sp|P62781|H4_PSAMI Histone H4 gb|AAA69664.1| histone gb|AAA30024.1| histone H4 gb|AAA30002.1| histone H4 prf||2209257B histone H4 7.0E-40
blast AAB00649 Histone protein 60 [Caenorhabditis elegans] ref|NP_501203.1| F55G1.11 [Caenorhabditis elegans] pir||T29230 hypothetical protein F55G1.11 - Caenorhabditis elegans 7.0E-40
blast HSUR4P histone H4, embryonic - sea urchin (Strongylocentrotus purpuratus) pir||HSUR4 histone H4 - sea urchin (Psammechinus miliaris) pir||S68537 histone H4 - starfish (Asterina pectinifera) gb|AAA30054.1| H4 histone protein 7.0E-40
Protein Domains
Database ID Description E-Value
Pfam IPR007125 EMBL-EBI Histone core 5.3E-13
Sequence
Target Sequence 
>C. ELEGANS:188963_AT
cgccatcgtaaagtccttcgggacaacatccaaggaatcacgaagccagccattcgccgt
ctagcaagaagaggaggagtcaaacgtatctctggattgatctacgaagaaactcgtgga
gttctcaaggtgttccttgagaacgttatccgagacgccgtcacttactgcgagcacgcc
aagaggaagaccgtcacagccatggacgttgtctatgctctgaagagac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGCCATCGTAAAGTCCTTCGGGACA 560 257 64 Antisense
GTAAAGTCCTTCGGGACAACATCCA 507 461 71 Antisense
AGGAATCACGAAGCCAGCCATTCGC 17 55 96 Antisense
GCCATTCGCCGTCTAGCAAGAAGAG 338 281 112 Antisense
TTCGCCGTCTAGCAAGAAGAGGAGG 313 695 116 Antisense
GATTGATCTACGAAGAAACTCGTGG 104 429 158 Antisense
GTTCCTTGAGAACGTTATCCGAGAC 364 439 195 Antisense
CCTTGAGAACGTTATCCGAGACGCC 17 269 198 Antisense
CACGCCAAGAGGAAGACCGTCACAG 641 199 238 Antisense
AGAGGAAGACCGTCACAGCCATGGA 176 69 245 Antisense
GACGTTGTCTATGCTCTGAAGAGAC 182 379 268 Antisense
Sequence Source Affymetrix Proprietary Database