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[188949_s_at] ThiF family

Description W02A11.4A /REP_DB=WormBase Gene ID /WP=CE25130 /TR=Q9XUM7 /GB=CAB04891.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=ThiF family
Cluster 6grp_total > 60      4grp_total > 20      6grp_cgc > 94      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 9.9
(-2.6)
A 		14.9
(-1.9)
A 		6.5
(-3)
A 		52
(3)
A 		46
B 2.5
(-3)
A 		12.4
(3)
A 		3.5
(-2.4)
A 		3.9
(-2.2)
A 		10
C 34.3
(3)
A 		13.8
(-0.8)
A 		2.3
(-3)
A 		23.4
(1)
A 		32
avr 15.6
(0.1) 		13.7
(-0.4) 		4.1
(-3) 		26.4
(3) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 28.6
(2.1)
A 		5.8
(-3)
A 		32.8
(3)
A 		25.3
(1.3)
A 		14.2
(-1.1)
A 		9.1
(-2.3)
A 		27
B 2
(-3)
A 		4.6
(1.3)
A 		3.6
(-0.3)
A 		3.7
(-0.2)
A 		3.7
(-0.2)
A 		5.6
(3)
A 		4
C 6.5
(-2.1)
A 		2.8
(-3)
A 		4.3
(-2.6)
A 		27
(3)
A 		4.1
(-2.7)
A 		6.4
(-2.1)
A 		24
avr 12.4
(0.4) 		4.4
(-3) 		13.6
(0.9) 		18.7
(3) 		7.3
(-1.8) 		7
(-1.9) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 3.6
(-1.1)
A 		3.2
(-1.5)
A 		1.7
(-3)
A 		7.6
(3)
A 		6
B 0.8
(-3)
A 		3.1
(3)
A 		0.8
(-3)
A 		0.8
(-3)
A 		2
C 11.4
(3)
A 		4
(-1.2)
A 		0.8
(-3)
A 		4
(-1.2)
A 		11
avr 5.3
(3) 		3.4
(0.3) 		1.1
(-3) 		4.1
(1.3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6
1 2 3 4 5 6 range
A 5.3
(0.8)
A 		2.1
(-3)
A 		7.1
(3)
A 		6.4
(2.2)
A 		4.3
(-0.4)
A 		2.2
(-2.9)
A 		5
B 0.7
(-3)
A 		1.5
(0.4)
A 		0.9
(-2.1)
A 		1
(-1.7)
A 		1.5
(0.4)
A 		2.1
(3)
A 		1
C 2.4
(-1)
A 		0.9
(-3)
A 		1.1
(-2.7)
A 		5.4
(3)
A 		1.3
(-2.5)
A 		2.1
(-1.4)
A 		5
avr 2.8
(-0.2) 		1.5
(-3) 		3
(0.2) 		4.3
(3) 		2.4
(-1.1) 		2.1
(-1.7) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188949_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.962
Sequence Type Exemplar sequence
Representative Public ID W02A11.4A NCBI
Target Description W02A11.4A /REP_DB=WormBase Gene ID /WP=CE25130 /TR=Q9XUM7 /GB=CAB04891.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=ThiF family
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026477(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026477 NCBI Caenorhabditis elegans UBA (human ubiquitin) related family member (uba-2) (uba-2) mRNA, complete cds. 9/11 None
SNAP00000019299 ENSEMBL cdna:SNAP chromosome:CEL140:I:12748770:12758842:1 9/11 None
W02A11.4 ENSEMBL cdna:known chromosome:CEL140:I:12749726:12753422:1 gene:W02A11.4 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000019310 1/11 Cross Hyb Matching Probes None
GENEFINDER00000019311 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12749656-12753353(+) 100.0 100.0
Public Domain and Genome References
Gene Title ThiF family
Gene Symbol uba-2
Ensembl W02A11.4
Entrez Gene 173145 Entrez gene
SwissProt Q9NAN1 EMBL-EBI
WormBase CE34382 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:13863_AT SUMO activating enzyme 2 (SAE2) at
ATH1-121501:263747_AT SUMO activating enzyme 2 (SAE2) at
CANINE:1604050_AT similar to Ubiquitin-like 1 activating enzyme E1B (SUMO-1 activating enzyme subunit 2) (Anthracycline-associated resistance ARX) cfa
CANINE_2:CFA.20940.1.S1_S_AT similar to Ubiquitin-like 1 activating enzyme E1B (SUMO-1 activating enzyme subunit 2) (Anthracycline-associated resistance ARX) cfa
DROSOPHILA_2:1631381_AT Smt3 activating enzyme 2 dm
CHICKEN:GGAAFFX.12210.1.S1_AT similar to Ubiquitin-like 2 activating enzyme E1B (SUMO-1 activating enzyme subunit 2) (Anthracycline-associated resistance ARX) (HRIHFB2115) gga
CHICKEN:GGAAFFX.12210.1.S1_S_AT similar to Ubiquitin-like 2 activating enzyme E1B (SUMO-1 activating enzyme subunit 2) (Anthracycline-associated resistance ARX) (HRIHFB2115) gga
HG-U133A:201177_S_AT SUMO-1 activating enzyme subunit 2 hs
HG-U133A_2:201177_S_AT SUMO-1 activating enzyme subunit 2 hs
HG-U133_PLUS_2:201177_S_AT SUMO-1 activating enzyme subunit 2 hs
HG-FOCUS:201177_S_AT SUMO-1 activating enzyme subunit 2 hs
HG-U133_PLUS_2:229587_AT SUMO-1 activating enzyme subunit 2 hs
HG-U133B:229587_AT SUMO-1 activating enzyme subunit 2 hs
HG-U95AV2:34814_AT SUMO-1 activating enzyme subunit 2 hs
HU35KSUBA:AA031993_AT SUMO-1 activating enzyme subunit 2 hs
HU35KSUBA:RC_AA278400_F_AT SUMO-1 activating enzyme subunit 2 hs
HU35KSUBD:RC_H11320_S_AT SUMO-1 activating enzyme subunit 2 hs
U133_X3P:G4885648_3P_X_AT SUMO-1 activating enzyme subunit 2 hs
U133_X3P:G4885648_3P_A_AT SUMO-1 activating enzyme subunit 2 hs
U133_X3P:HS.4311.1.A1_3P_AT SUMO-1 activating enzyme subunit 2 hs
HG-U95E:74323_AT SUMO-1 activating enzyme subunit 2 hs
MG-U74AV2:96701_AT ubiquitin-like 1 (sentrin) activating enzyme E1B mm
MOE430A:1433440_X_AT ubiquitin-like 1 (sentrin) activating enzyme E1B mm
MOUSE430A_2:1433440_X_AT ubiquitin-like 1 (sentrin) activating enzyme E1B mm
MOUSE430_2:1433440_X_AT ubiquitin-like 1 (sentrin) activating enzyme E1B mm
MOE430A:1437278_A_AT ubiquitin-like 1 (sentrin) activating enzyme E1B mm
MOUSE430A_2:1437278_A_AT ubiquitin-like 1 (sentrin) activating enzyme E1B mm
MOUSE430_2:1437278_A_AT ubiquitin-like 1 (sentrin) activating enzyme E1B mm
MOE430A:1416280_AT ubiquitin-like 1 (sentrin) activating enzyme E1B mm
MOUSE430A_2:1416280_AT ubiquitin-like 1 (sentrin) activating enzyme E1B mm
MOUSE430_2:1416280_AT ubiquitin-like 1 (sentrin) activating enzyme E1B mm
MOE430A:1448283_A_AT ubiquitin-like 1 (sentrin) activating enzyme E1B mm
MOUSE430A_2:1448283_A_AT ubiquitin-like 1 (sentrin) activating enzyme E1B mm
MOUSE430_2:1448283_A_AT ubiquitin-like 1 (sentrin) activating enzyme E1B mm
MU19KSUBC:TC40975_AT Ubiquitin-like 1 (sentrin) activating enzyme E1B (Uble1b), mRNA mm
MU11KSUBB:W15768_AT Ubiquitin-like 1 (sentrin) activating enzyme E1B (Uble1b), mRNA mm
RG-U34C:RC_AI102437_AT ubiquitin-like 1 (sentrin) activating enzyme E1B (predicted) rn
RG-U34C:RC_AI176509_AT ubiquitin-like 1 (sentrin) activating enzyme E1B (predicted) rn
RAE230A:1376212_AT ubiquitin-like 1 (sentrin) activating enzyme E1B (predicted) rn
RAT230_2:1376212_AT ubiquitin-like 1 (sentrin) activating enzyme E1B (predicted) rn
RAE230A:1371657_AT ubiquitin-like 1 (sentrin) activating enzyme E1B (predicted) rn
RAT230_2:1371657_AT ubiquitin-like 1 (sentrin) activating enzyme E1B (predicted) rn
YEAST_2:1771475_AT Nuclear protein that acts as a heterodimer with Aos1p to activate Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
4839 ubiquitin activating enzyme activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB54319 Hypothetical protein W02A11.4 [Caenorhabditis elegans] ref|NP_001021648.1| UBA (human ubiquitin) related family member (uba-2) [Caenorhabditis elegans] 0.0
blast T26071 hypothetical protein W02A11.4a - Caenorhabditis elegans 0.0
blast CAB54319 Hypothetical protein W02A11.4 [Caenorhabditis elegans] ref|NP_001021648.1| UBA (human ubiquitin) related family member (uba-2) [Caenorhabditis elegans] 1.0E-174
blast T26071 hypothetical protein W02A11.4a - Caenorhabditis elegans 1.0E-168
blast CAE67994 Hypothetical protein CBG13604 [Caenorhabditis briggsae] 1.0E-144
blast CAE67994 Hypothetical protein CBG13604 [Caenorhabditis briggsae] 1.0E-111
Protein Domains
Database ID Description E-Value
Pfam IPR000127 EMBL-EBI Ubiquitin-activating enzyme repeat 1.0E-33
Pfam IPR000127 EMBL-EBI Ubiquitin-activating enzyme repeat 1.0E-33
Pfam IPR000594 EMBL-EBI UBA/THIF-type NAD/FAD binding fold 1.7E-44
Sequence
Target Sequence 
>C. ELEGANS:188949_S_AT
gcgccagttggaacacgtcaatgggctgaaaaaaagctccattttcacactcaaaaaata
aatgttttccacccaaaaaacctcctaaaaccccaaaattttcagcttttcctgcacgat
atcgaatatctttgcaaaatggaacacctttggaaacaacgaaaacgtccatcaccactg
gaattccatacagcttcatctaccggaggagagcctcaatccctgtgtgatgctcaacgt
gatgatacttcaatttggacactgtccacgtgtgcaaaagtcttctccacgtgtattcaa
gagctcttggagcaaatacgggcggagccagacgtgaagctggcttttgataaagatcat
gcaattattatgtcatttgtggctgcttgtgccaatattcgagcgaaaatctttgggatt
ccgatgaaatcacaatttgatattaaagcaatggctggaaatattattccagcaattgca
tcaactaatgcaattgttgctggaattattgttactgaggctgtgagggtgattgaagga
tcaacggtgatttgtaattcgagtattgcgacgactcagtcgaatccgcgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGCCAGTTGGAACACGTCAATGGG 149 287 760 Antisense
AAAAAGCTCCATTTTCACACTCAAA 639 129 790 Antisense
AATTTTCAGCTTTTCCTGCACGATA 497 175 856 Antisense
ACAACGAAAACGTCCATCACCACTG 203 111 915 Antisense
GGAATTCCATACAGCTTCATCTACC 578 533 939 Antisense
GCTTCATCTACCGGAGGAGAGCCTC 517 305 952 Antisense
GAGCCTCAATCCCTGTGTGATGCTC 659 385 970 Antisense
GTCCACGTGTGCAAAAGTCTTCTCC 367 477 1023 Antisense
GTCTTCTCCACGTGTATTCAAGAGC 586 467 1039 Antisense
GGCTGCTTGTGCCAATATTCGAGCG 682 539 1140 Antisense
GCGACGACTCAGTCGAATCCGCGTG 566 289 1327 Antisense
Sequence Source Affymetrix Proprietary Database