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[188939_at] Engrailed homeotic protein

Description C13G5.1 /REP_DB=WormBase Gene ID /WP=CE06819 /GEN=ceh-16 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Engrailed homeotic protein
Cluster 4grp_high_8_8 > 37      6grp_total > 30      4grp_total > 42      6grp_cgc > 62      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 194
1 2 3 4 range
A 180
(-3)
P 		328
(3)
P 		197.2
(-2.3)
A 		237.2
(-0.7)
P 		148
B 195.7
(-3)
P 		374.4
(3)
P 		250.6
(-1.2)
P 		228.7
(-1.9)
P 		179
C 190
(-3)
P 		288.8
(3)
P 		252.2
(0.8)
P 		242.1
(0.2)
P 		99
avr 188.6
(-3) 		330.4
(3) 		233.3
(-1.1) 		236
(-1) 		142

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 238
1 2 3 4 5 6 range
A 218.9
(0.5)
M 		293.7
(3)
P 		225.7
(0.8)
P 		174.9
(-0.9)
P 		155.3
(-1.6)
A 		111.5
(-3)
A 		182
B 198.2
(-0.5)
P 		335.3
(3)
P 		267
(1.3)
P 		264.4
(1.2)
P 		111.9
(-2.6)
A 		97.2
(-3)
A 		238
C 154.3
(-1.4)
P 		309.1
(3)
P 		209.9
(0.2)
P 		259.4
(1.6)
P 		106.7
(-2.8)
M 		98.7
(-3)
A 		210
avr 190.5
(-0.5) 		312.7
(3) 		234.2
(0.8) 		232.9
(0.7) 		124.6
(-2.4) 		102.5
(-3) 		210
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 65.5
(2.1)
P 		71.2
(3)
P 		51.5
(-0.2)
A 		34.7
(-3)
P 		37
B 62.4
(-1.1)
P 		92.5
(3)
P 		58.1
(-1.7)
P 		48.7
(-3)
P 		44
C 62.9
(-0.2)
P 		83
(2.4)
P 		87.9
(3)
P 		40.8
(-3)
P 		47
avr 63.6
(0.3) 		82.2
(3) 		65.8
(0.6) 		41.4
(-3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 40.3
(-2)
M 		104.5
(3)
P 		49.2
(-1.3)
P 		44.4
(-1.7)
P 		46.9
(-1.5)
A 		27.2
(-3)
A 		77
B 69
(-0.4)
P 		111.6
(3)
P 		64.8
(-0.7)
P 		70
(-0.3)
P 		45.5
(-2.3)
A 		36.3
(-3)
A 		75
C 57.2
(-0.6)
P 		94.3
(3)
P 		53.4
(-1)
P 		52.2
(-1.1)
P 		34.5
(-2.9)
M 		33
(-3)
A 		61
avr 55.5
(-1) 		103.5
(3) 		55.8
(-1) 		55.5
(-1) 		42.3
(-2.2) 		32.2
(-3) 		71
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188939_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7935
Sequence Type Exemplar sequence
Representative Public ID C13G5.1 NCBI
Target Description C13G5.1 /REP_DB=WormBase Gene ID /WP=CE06819 /GEN=ceh-16 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Engrailed homeotic protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066497(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066497 NCBI Caenorhabditis elegans C.Elegans Homeobox family member (ceh-16) (ceh-16) mRNA, complete cds. 11/11 None
GENEFINDER00000030087 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8622791:8625903:1 9/11 None
C13G5.1 ENSEMBL cdna:known chromosome:CEL140:III:8622561:8625903:1 gene:C13G5.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000030084 7/11 Cross Hyb Matching Probes None
SNAP00000030085 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8622555-8625898(+) 100.0 100.0
Public Domain and Genome References
Gene Title Engrailed homeotic protein
Gene Symbol ceh-16
Ensembl C13G5.1
Entrez Gene 191618 Entrez gene
SwissProt P34326 EMBL-EBI
WormBase CE37477 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1627445_S_AT engrailed dm
DROSGENOME1:153867_AT engrailed dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB37562 C.elegans homeobox protein 16 [Caenorhabditis elegans] ref|NP_498898.2| C.Elegans Homeobox family member (ceh-16) [Caenorhabditis elegans] sp|P34326|HM16_CAEEL Homeobox protein engrailed-like ceh-16 gb|AAT67384.1| homeobox gene 16 [Caenorhabditis elegans] 3.0E-75
blast CAE75016 Hypothetical protein CBG22920 [Caenorhabditis briggsae] 2.0E-40
blast CAG04300 unnamed protein product [Tetraodon nigroviridis] 3.0E-27
Protein Domains
Database ID Description E-Value
scop a.4.1.Homeodomain All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Homeodomain 9.99999968265523E-21
Pfam IPR001356 EMBL-EBI Homeobox 1.7E-29
Sequence
Target Sequence 
>C. ELEGANS:188939_AT
gagtttgtgttgtatctccgttcactgaccgaaatgattctgaaattcggtattgaacga
atcctgtcttccccctacccttgcccatctccaactatttcaactcctgctacaagtcca
tcaagtatttctcctacttttgcatctccaaatggaacaccaaatattgcgtcaagtatg
tatcctgcatgggtgttcagcacaagatattccgatcgaccatctgcaggccctcgacat
cgaaaatcccgaaaacgagaatcaactggaagtagtggaagttcagaagaagaaaaaaga
ccacgtactgcattcactggagatcaattggaccggctcaaaactgaattccgggaaagc
agatatctaacagaaaagcggcgacaagaattggctcacgagctcggactcaacgaatcg
cagatcaaaatctggttccagaacaagcgtgccaaactcaaaaagtcgacatcttctgtt
ccaagagatcgatgctcctccgtcactccaaatcctcataatcatccgtcaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGTTTGTGTTGTATCTCCGTTCAC 276 399 28 Antisense
ATCTCCGTTCACTGACCGAAATGAT 652 33 41 Antisense
GGTATTGAACGAATCCTGTCTTCCC 424 505 76 Antisense
AACTCCTGCTACAAGTCCATCAAGT 68 143 129 Antisense
TCTCCTACTTTTGCATCTCCAAATG 242 611 157 Antisense
GTATCCTGCATGGGTGTTCAGCACA 338 455 207 Antisense
AAGATATTCCGATCGACCATCTGCA 521 155 231 Antisense
AAAAGACCACGTACTGCATTCACTG 334 131 322 Antisense
ATTGGCTCACGAGCTCGGACTCAAC 276 3 417 Antisense
TTCCAAGAGATCGATGCTCCTCCGT 709 699 506 Antisense
CAAATCCTCATAATCATCCGTCAAT 592 193 536 Antisense
Sequence Source Affymetrix Proprietary Database