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[188936_at] Ras family

Description F54C8.5 /REP_DB=WormBase Gene ID /WP=CE00191 /TR=SW:P34443 /GB=CAA80155.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Ras family
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 73      4grp_highest_8_8 > 46      4grp_high_8_8 > 10      6grp_highest_nonorm > 91      6grp_total > 70      4grp_total > 22      6grp_highest_sub > 03_04      6grp_high1_sub > 03_04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 166
1 2 3 4 range
A 288.5
(2.8)
P 		127.5
(-3)
P 		157.3
(-1.9)
P 		293.5
(3)
P 		166
B 231.5
(3)
P 		151.8
(-2.1)
P 		138
(-3)
P 		214.4
(1.9)
P 		94
C 233.4
(1.2)
P 		161.8
(-2.2)
P 		145.8
(-3)
P 		272.1
(3)
P 		126
avr 251.1
(2.5) 		147
(-3) 		147
(-3) 		260
(3) 		113

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 119
1 2 3 4 5 6 range
A 182.2
(-2.7)
P 		178.7
(-3)
P 		260.5
(3)
P 		243.1
(1.7)
P 		248.9
(2.1)
P 		181.2
(-2.8)
P 		82
B 195
(-3)
P 		264.8
(2.2)
P 		241
(0.4)
P 		276.1
(3)
P 		199.2
(-2.7)
P 		241.5
(0.4)
P 		81
C 157.4
(-3)
P 		262.7
(3)
P 		180.1
(-1.7)
P 		246.1
(2.1)
P 		164.4
(-2.6)
P 		252
(2.4)
P 		105
avr 178.2
(-3) 		235.4
(1.5) 		227.2
(0.8) 		255.1
(3) 		204.2
(-1) 		224.9
(0.6) 		77
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 77
1 2 3 4 range
A 105
(3)
P 		27.7
(-3)
P 		41.1
(-2)
P 		43
(-1.8)
P 		77
B 73.8
(3)
P 		37.5
(-2.2)
P 		32
(-3)
P 		45.7
(-1)
P 		42
C 77.3
(3)
P 		46.5
(-2.9)
P 		50.8
(-2.1)
P 		45.9
(-3)
P 		31
avr 85.4
(3) 		37.2
(-3) 		41.3
(-2.5) 		44.9
(-2) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 57
1 2 3 4 5 6 range
A 33.6
(-3)
P 		63.6
(1.3)
P 		56.7
(0.3)
P 		61.7
(1.1)
P 		75.1
(3)
P 		44.1
(-1.5)
P 		42
B 67.8
(-1.2)
P 		88.1
(2.6)
P 		58.5
(-3)
P 		73.1
(-0.2)
P 		80.9
(1.3)
P 		90.1
(3)
P 		32
C 58.3
(-1.1)
P 		80.1
(2.3)
P 		45.9
(-3)
P 		49.5
(-2.4)
P 		53.2
(-1.9)
P 		84.4
(3)
P 		39
avr 53.2
(-3) 		77.3
(3) 		53.7
(-2.9) 		61.4
(-1) 		69.7
(1.1) 		72.9
(1.9) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188936_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6154
Sequence Type Exemplar sequence
Representative Public ID F54C8.5 NCBI
Target Description F54C8.5 /REP_DB=WormBase Gene ID /WP=CE00191 /TR=SW:P34443 /GB=CAA80155.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Ras family
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066678(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066678 NCBI Caenorhabditis elegans F54C8.5 (F54C8.5) mRNA, complete cds. 11/11 None
GENEFINDER00000014867 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9454606:9455926:-1 11/11 None
F54C8.5 ENSEMBL cdna:known chromosome:CEL140:III:9454128:9455910:-1 gene:F54C8.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000014866 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9454601-9455901(-) 100.0 100.0
Public Domain and Genome References
Gene Title Ras family
Ensembl F54C8.5
Entrez Gene 176327 Entrez gene
SwissProt P34443 EMBL-EBI
WormBase CE00191 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1584599_S_AT similar to GTP-binding protein Rheb (Ras homolog enriched in brain) cfa
CANINE_2:CFA.4395.1.A1_S_AT similar to GTP-binding protein Rheb (Ras homolog enriched in brain) cfa
DROSOPHILA_2:1630949_S_AT UAS construct of Saucedo dm
DROSGENOME1:152298_AT UAS construct of Saucedo dm
CHICKEN:GGAAFFX.3865.1.S1_AT similar to RAS-homolog enriched in brain gga
HG-U133_PLUS_2:201451_X_AT Ras homolog enriched in brain hs
HG-U133A_2:201451_X_AT Ras homolog enriched in brain hs
HG-U133A:201451_X_AT Ras homolog enriched in brain hs
HG-U133A_2:201452_AT Ras homolog enriched in brain hs
HG-U133_PLUS_2:201452_AT Ras homolog enriched in brain hs
HG-U133A:201452_AT Ras homolog enriched in brain hs
HG-U133_PLUS_2:201453_X_AT Ras homolog enriched in brain hs
HG-U133A_2:201453_X_AT Ras homolog enriched in brain hs
HG-U133A:201453_X_AT Ras homolog enriched in brain hs
U133_X3P:201453_3P_X_AT Ras homolog enriched in brain hs
HG-U95AV2:1659_S_AT Ras homolog enriched in brain hs
HC-G110:1659_S_AT Ras homolog enriched in brain hs
HUGENEFL:D78132_S_AT Ras homolog enriched in brain hs
U133_X3P:G5032040_3P_X_AT Ras homolog enriched in brain hs
U133_X3P:G5032040_3P_A_AT Ras homolog enriched in brain hs
U133_X3P:HS2.279903.2.S1_3P_S_AT Ras homolog enriched in brain hs
U133_X3P:HS.279903.2.S1_3P_X_AT Ras homolog enriched in brain hs
U133_X3P:HS.279903.2.S1_3P_A_AT Ras homolog enriched in brain hs
U133_X3P:HS.279903.2.A1_3P_A_AT Ras homolog enriched in brain hs
U133_X3P:HS.279903.1.S1_3P_AT Ras homolog enriched in brain hs
U133_X3P:HS.279903.0.S1_3P_AT Ras homolog enriched in brain hs
U133_X3P:213404_3P_S_AT Ras homolog enriched in brain hs
U133_X3P:1555780_3P_A_AT Ras homolog enriched in brain hs
HG-U133_PLUS_2:1555780_A_AT Ras homolog enriched in brain hs
HG-U133A_2:213404_S_AT Ras homolog enriched in brain hs
HG-U133_PLUS_2:213404_S_AT Ras homolog enriched in brain hs
HG-U133A:213404_S_AT Ras homolog enriched in brain hs
HG-U133A_2:213409_S_AT Ras homolog enriched in brain hs
HG-FOCUS:213409_S_AT Ras homolog enriched in brain hs
HG-U133_PLUS_2:213409_S_AT Ras homolog enriched in brain hs
HG-U133A:213409_S_AT Ras homolog enriched in brain hs
HG-U133_PLUS_2:227633_AT Ras homolog enriched in brain hs
HG-U133B:227633_AT Ras homolog enriched in brain hs
HG-U95AV2:40627_AT Ras homolog enriched in brain hs
HG-U95B:56845_AT Ras homolog enriched in brain hs
HG-U95E:78322_R_AT Ras homolog enriched in brain hs
U133_X3P:HS.129827.0.A1_3P_AT Ras homolog enriched in brain hs
HG-U133_PLUS_2:241795_AT Ras homolog enriched in brain hs
HG-U133B:241795_AT Ras homolog enriched in brain hs
HG-U95D:77655_R_AT Ras homolog enriched in brain hs
HG-U133_PLUS_2:243008_AT Ras homolog enriched in brain hs
HG-U133B:243008_AT Ras homolog enriched in brain hs
MU11KSUBB:MSA.4071.0_F_AT RAS-homolog enriched in brain mm
MG-U74BV2:165370_AT RAS-homolog enriched in brain mm
MOE430A:1416636_AT RAS-homolog enriched in brain mm
MOUSE430A_2:1416636_AT RAS-homolog enriched in brain mm
MOUSE430_2:1416636_AT RAS-homolog enriched in brain mm
MU19KSUBB:TC33925_AT RAS-homolog enriched in brain (Rheb), mRNA mm
YEAST_2:1776368_AT Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA80155 Hypothetical protein F54C8.5 [Caenorhabditis elegans] ref|NP_499079.1| F54C8.5 [Caenorhabditis elegans] sp|P34443|RASL_CAEEL Ras-like protein F54C8.5 1.0E-108
blast CAE65213 Hypothetical protein CBG10088 [Caenorhabditis briggsae] 6.0E-76
blast CAE65213 Hypothetical protein CBG10088 [Caenorhabditis briggsae] 7.0E-76
blast AAH59505 Hypothetical protein MGC73144 [Danio rerio] ref|NP_957023.1| hypothetical protein LOC393702 [Danio rerio] 4.0E-38
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 9.5E-73
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 9.5E-73
Sequence
Target Sequence 
>C. ELEGANS:188936_AT
aagtggccgttatgggatatcctcatgttggaaaatcggcgttggtacttcgtttcacac
aaaatattttccctgaaaggtatgaatcaacaattgaggatcaacattcgaagcacatag
cagcgtttcacagggactatcatttaagagtcactgacacagcaggacaacaagaataca
cagttttcccgagaagttgttcccttgatattaatggatttatacttgtttatgcaatcg
atgatagaaaatcattcgaaatgtgcagcaacatctatgaaaaaatcgtaagaacctatg
gtgacacaagcattccaattgtaattgttggaaacaagacggatctcagcacgcaaaggg
tcgttcgagcagaagaaggtgaagaacttgctagacaatgggatgcgaagtttgtcgaga
tcacggccagagaatcgaatcgagtgcatgaggtgttcgaattgcttctccgtgagatag
aaatctcgcgtggaaatctatcaccaacggagcgtccaaacggtaattcacca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGTGGCCGTTATGGGATATCCTCA 174 157 56 Antisense
GTTGGTACTTCGTTTCACACAAAAT 448 295 96 Antisense
GCAGCGTTTCACAGGGACTATCATT 696 313 175 Antisense
GAGTCACTGACACAGCAGGACAACA 270 399 203 Antisense
TTTCCCGAGAAGTTGTTCCCTTGAT 463 669 240 Antisense
ACAAGACGGATCTCAGCACGCAAAG 5 115 389 Antisense
GCACGCAAAGGGTCGTTCGAGCAGA 291 315 404 Antisense
GAAGTTTGTCGAGATCACGGCCAGA 238 337 462 Antisense
TGAGGTGTTCGAATTGCTTCTCCGT 45 569 504 Antisense
GAAATCTATCACCAACGGAGCGTCC 193 359 548 Antisense
GGAGCGTCCAAACGGTAATTCACCA 489 521 564 Antisense
Sequence Source Affymetrix Proprietary Database