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[188931_s_at]

Description F54E7.3 /REP_DB=WormBase Gene ID /WP=CE25905 /CHR=3 /FEA=Sanger Annotation /DEF=locus:par-3 (ST.LOUIS) TR:Q20786 protein_id:AAC77513.1
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 59      4grp_highest_8_8 > 60      4grp_high_8_8 > 25      6grp_highest_nonorm > 85      6grp_total > 49      4grp_total > 38      6grp_highest_sub > Cu      6grp_cgc > 09      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 495
1 2 3 4 range
A 736.8
(3)
P 		483.3
(-1.3)
P 		384.8
(-3)
P 		557.5
(-0.1)
P 		352
B 605.1
(3)
P 		436.4
(-0.4)
P 		310.5
(-3)
P 		440
(-0.4)
P 		295
C 805.6
(3)
P 		416.2
(-2.3)
P 		368.2
(-3)
P 		477.9
(-1.5)
P 		437
avr 715.8
(3) 		445.3
(-1.5) 		354.5
(-3) 		491.8
(-0.7) 		361

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 896
1 2 3 4 5 6 range
A 880.4
(0.1)
P 		979.3
(0.8)
P 		413.7
(-3)
P 		745.1
(-0.8)
P 		815.9
(-0.3)
P 		1309.8
(3)
P 		896
B 929.8
(2.2)
P 		576.4
(-2.2)
P 		507.2
(-3)
P 		633.6
(-1.4)
P 		674
(-1)
P 		996
(3)
P 		489
C 939.4
(3)
P 		678.9
(-3)
P 		705.2
(-2.4)
P 		702.1
(-2.5)
P 		914.3
(2.4)
P 		777.6
(-0.7)
P 		261
avr 916.5
(1.6) 		744.9
(-0.5) 		542
(-3) 		693.6
(-1.1) 		801.4
(0.2) 		1027.8
(3) 		486
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 196
1 2 3 4 range
A 268.3
(3)
P 		104.9
(-2.3)
P 		100.6
(-2.4)
P 		81.6
(-3)
P 		187
B 193
(3)
P 		107.8
(-1.2)
P 		72
(-3)
P 		93.8
(-1.9)
P 		121
C 266.7
(3)
P 		119.7
(-1.7)
P 		128.3
(-1.5)
P 		80.6
(-3)
P 		186
avr 242.7
(3) 		110.8
(-2) 		100.3
(-2.4) 		85.3
(-3) 		157

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 281
1 2 3 4 5 6 range
A 162.3
(-1.3)
P 		348.3
(3)
P 		90.1
(-3)
P 		189
(-0.7)
P 		246.2
(0.6)
P 		318.9
(2.3)
P 		258
B 323.5
(1.8)
P 		191.9
(-1.3)
P 		123.2
(-3)
P 		167.8
(-1.9)
P 		273.8
(0.6)
P 		371.4
(3)
P 		248
C 348.2
(3)
P 		207.1
(-1.1)
P 		179.5
(-1.9)
P 		141.2
(-3)
P 		295.9
(1.5)
P 		260.3
(0.5)
P 		207
avr 278
(1.7) 		249.1
(0.8) 		130.9
(-3) 		166
(-1.9) 		272
(1.6) 		316.9
(3) 		186
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188931_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7234
Sequence Type Exemplar sequence
Representative Public ID F54E7.3 NCBI
Target Description F54E7.3 /REP_DB=WormBase Gene ID /WP=CE25905 /CHR=3 /FEA=Sanger Annotation /DEF=locus:par-3 (ST.LOUIS) TR:Q20786 protein_id:AAC77513.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027436(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027436 NCBI Caenorhabditis elegans abnormal embryonic PARtitioning of cytoplasm family member (par-3) (par-3) mRNA, complete cds. 11/11 None
GENEFINDER00000020795 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5664322:5676345:1 11/11 None
SNAP00000020784 ENSEMBL cdna:SNAP chromosome:CEL140:III:5668490:5680097:1 10/11 None
F54E7.3b ENSEMBL cdna:known chromosome:CEL140:III:5664184:5676681:1 gene:F54E7.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_065816 5/11 Cross Hyb Matching Probes A 
F54E7.3a 5/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175783 2 1 NM_001027436 175616_at (11), 188931_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5664320-5676344(+) 100.0 100.0
Public Domain and Genome References
Ensembl F54E7.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK73875 Abnormal embryonic partitioning of cytoplasm protein 3, isoform b [Caenorhabditis elegans] ref|NP_001022607.1| abnormal embryonic PARtitioning of cytoplasm family member (par-3) [Caenorhabditis elegans] sp|Q17353|PARD3_CAEEL Partition defective-3 protein (Par-3 protein) (Abnormal embryonic partitioning of cytoplasm protein 3) gb|AAB18670.1| par-3 gene product 0.0
blast AAK73874 Abnormal embryonic partitioning of cytoplasm protein 3, isoform a [Caenorhabditis elegans] ref|NP_498217.2| abnormal embryonic PARtitioning of cytoplasm family member (par-3) [Caenorhabditis elegans] 0.0
blast T34302 cell polarity protein par-3 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.5E-6
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.2E-7
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 4.8E-11
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.5E-6
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.2E-7
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 4.8E-11
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.5E-6
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.2E-7
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 4.8E-11
Pfam IPR004038 EMBL-EBI Ribosomal protein L7Ae/L30e/S12e/Gadd45 8.1E-36
Sequence
Target Sequence 
>C. ELEGANS:188931_S_AT
gggacggagcacttcgagtggagccgttgcctctggatcatcatcaactgggttccaata
tgccgcgaaggagaagtatgcggatgctcgatctggaaaattcaatggtggatcgacccg
actctttattccaagacacggcggtgggctcagcgcggctgcgtttgcaacaaacttcgg
gggagaagcctacgagacacgtggtggaggagccggcggctcgccgagccaataccgtcg
cagagatcagggaccgcctcatcgttttccccaatttccaatagctgcctcatcaatgcg
tgtatcatcagcggcacaaccgcttgcgaattctgtaaacctacgatctgtatcaaataa
taaaatcaacgataattccgctgagcgaatctcattaagagccagaaagaagcttcatcg
agaagcagtaacacgaagcgagttcttcttgccaccgtcgtattcaaatgagaatatcaa
actgttcaacagtctccttccgccatccacatcaagtatcatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGACGGAGCACTTCGAGTGGAGCC 395 495 3864 Antisense
TCAACTGGGTTCCAATATGCCGCGA 15 631 3907 Antisense
GAAGTATGCGGATGCTCGATCTGGA 298 337 3936 Antisense
TTCAATGGTGGATCGACCCGACTCT 218 685 3964 Antisense
GAGCCAATACCGTCGCAGAGATCAG 177 385 4089 Antisense
ATAGCTGCCTCATCAATGCGTGTAT 142 23 4144 Antisense
AATGCGTGTATCATCAGCGGCACAA 379 167 4158 Antisense
GGCACAACCGCTTGCGAATTCTGTA 538 535 4176 Antisense
ACACGAAGCGAGTTCTTCTTGCCAC 665 109 4294 Antisense
TTCTTGCCACCGTCGTATTCAAATG 419 689 4309 Antisense
TCCGCCATCCACATCAAGTATCATG 702 595 4362 Antisense
Sequence Source Affymetrix Proprietary Database