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[188922_at] 14-3-3 protein

Description M117.3 /REP_DB=WormBase Gene ID /WP=CE06201 /TR=Q21539 /GB=CAA98135.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=14-3-3 protein
Cluster 6grp_total > 76      4grp_total > 02      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 116
1 2 3 4 range
A 4.3
(-2.9)
A 		10.6
(-2.6)
A 		2.9
(-3)
A 		117.8
(3)
P 		115
B 7.4
(-2.5)
A 		2.9
(-3)
A 		8.8
(-2.3)
A 		56.2
(3)
P 		53
C 12.8
(-2)
A 		9.1
(-2.4)
A 		1.8
(-3)
A 		69.8
(3)
P 		68
avr 8.2
(-2.7) 		7.5
(-2.8) 		4.5
(-3) 		81.3
(3) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 9.3
(-2)
A 		4.5
(-3)
A 		34.3
(3)
A 		21
(0.3)
A 		13.1
(-1.3)
A 		29.3
(2)
A 		30
B 11.3
(-2.1)
A 		5.2
(-3)
A 		12.9
(-1.9)
A 		6
(-2.9)
A 		11
(-2.2)
A 		48.1
(3)
P 		43
C 5.4
(-2.5)
A 		6.5
(-2.3)
A 		2.3
(-3)
A 		15.8
(-0.6)
A 		5
(-2.5)
A 		36.5
(3)
A 		34
avr 8.7
(-2.4) 		5.4
(-3) 		16.5
(-1) 		14.3
(-1.4) 		9.7
(-2.2) 		38
(3) 		33
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 1.6
(-2.7)
A 		2.3
(-2.5)
A 		0.8
(-3)
A 		17.2
(3)
P 		16
B 2.4
(-2.1)
A 		0.7
(-3)
A 		2
(-2.3)
A 		12
(3)
P 		11
C 4.2
(-1.1)
A 		2.6
(-1.9)
A 		0.6
(-3)
A 		11.8
(3)
P 		11
avr 2.7
(-2.2) 		1.9
(-2.6) 		1.1
(-3) 		13.7
(3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 1.7
(-2.9)
A 		1.6
(-3)
A 		7.5
(3)
A 		5.3
(0.8)
A 		4
(-0.6)
A 		7.1
(2.6)
A 		6
B 3.9
(-2.2)
A 		1.7
(-3)
A 		3.1
(-2.4)
A 		1.6
(-3)
A 		4.5
(-1.9)
A 		17.9
(3)
P 		16
C 2
(-2.3)
A 		2
(-2.3)
A 		0.6
(-3)
A 		3.2
(-1.7)
A 		1.6
(-2.5)
A 		12.2
(3)
A 		12
avr 2.5
(-2.6) 		1.8
(-3) 		3.7
(-1.9) 		3.4
(-2.1) 		3.4
(-2.1) 		12.4
(3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188922_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8460
Sequence Type Exemplar sequence
Representative Public ID M117.3 NCBI
Target Description M117.3 /REP_DB=WormBase Gene ID /WP=CE06201 /TR=Q21539 /GB=CAA98135.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=14-3-3 protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069835(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069835 NCBI Caenorhabditis elegans M117.3 (M117.3) mRNA, complete cds. 11/11 None
SNAP00000015385 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11823486:11823908:1 11/11 None
GENEFINDER00000015392 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11823486:11823908:1 11/11 None
M117.3 ENSEMBL cdna:known chromosome:CEL140:IV:11823486:11823908:1 gene:M117.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11823491-11823914(+) 100.0 100.0
Public Domain and Genome References
Gene Title 14-3-3 protein
Ensembl M117.3
Entrez Gene 187477 Entrez gene
SwissProt Q21539 EMBL-EBI
WormBase CE06201 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
19904 protein domain specific binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98135 Hypothetical protein M117.3 [Caenorhabditis elegans] ref|NP_502236.1| M117.3 [Caenorhabditis elegans] 2.0E-65
blast P41932 14-3-3-like protein 1 gb|AAA61872.1| 14-3-3 protein 6.0E-18
blast CAA98138 Hypothetical protein M117.2 [Caenorhabditis elegans] ref|NP_502235.1| abnormal embryonic PARtitioning of cytoplasm family member (par-5) [Caenorhabditis elegans] 6.0E-18
Protein Domains
Database ID Description E-Value
Pfam IPR000308 EMBL-EBI 14-3-3 protein 1.2E-20
Sequence
Target Sequence 
>C. ELEGANS:188922_AT
ggtcgccgaccattttaggtatctggtccaatacgacgatatcaacagagaagagcatgc
acacaagtcgcgaatcgcttatcaggaggctcttggtatcgctaaggacaagatgcagcc
aactcacccaattcgccttggacttgccctcaatgcaagcgccttaaacttcgatgtcct
gaatcttcccaaagaagccaacgaaattgcacagagtgctcttgatagtgctcatcgcga
attggagaagatgaagagctcactggattcgtacgatatctcgaacttgaaacgtgctcg
tacactttacgatctattgaagagtactcacgagcagcgtgtcgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTCGCCGACCATTTTAGGTATCTG 440 481 15 Antisense
GCATGCACACAAGTCGCGAATCGCT 534 309 69 Antisense
AATCGCTTATCAGGAGGCTCTTGGT 551 167 87 Antisense
TGGACTTGCCCTCAATGCAAGCGCC 561 545 153 Antisense
GCAAGCGCCTTAAACTTCGATGTCC 265 319 169 Antisense
TCGATGTCCTGAATCTTCCCAAAGA 390 603 185 Antisense
TGCTCTTGATAGTGCTCATCGCGAA 251 581 231 Antisense
AGAGCTCACTGGATTCGTACGATAT 428 69 269 Antisense
GATATCTCGAACTTGAAACGTGCTC 342 425 289 Antisense
AACGTGCTCGTACACTTTACGATCT 191 145 305 Antisense
GAGTACTCACGAGCAGCGTGTCGAC 177 399 336 Antisense
Sequence Source Affymetrix Proprietary Database