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[188921_at] dehydrogenase

Description F25D1.5 /REP_DB=WormBase Gene ID /WP=CE05726 /TR=Q19774 /GB=CAA98264.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=dehydrogenase
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 17      4grp_highest_8_8 > 51      4grp_high_8_8 > 24      6grp_highest_nonorm > 80      6grp_total > 07      4grp_total > 39      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 175
1 2 3 4 range
A 232.7
(3)
P 		130.1
(-1.7)
P 		101.6
(-3)
P 		148.3
(-0.9)
P 		131
B 223.7
(3)
P 		85
(-3)
P 		133.4
(-0.9)
P 		99.9
(-2.4)
P 		139
C 259.7
(3)
P 		89.6
(-3)
P 		118.4
(-2)
P 		153.3
(-0.8)
P 		170
avr 238.7
(3) 		101.6
(-3) 		117.8
(-2.3) 		133.8
(-1.6) 		137

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 461
1 2 3 4 5 6 range
A 512.9
(3)
P 		158.8
(-1.6)
P 		162.2
(-1.6)
P 		102.4
(-2.4)
P 		55.6
(-3)
P 		64.5
(-2.9)
P 		457
B 509
(3)
P 		197
(-1.1)
P 		144.6
(-1.8)
P 		84.6
(-2.6)
P 		74.7
(-2.8)
P 		57.8
(-3)
P 		451
C 441.2
(3)
P 		193.9
(-0.8)
P 		140.7
(-1.6)
P 		116.7
(-2)
P 		90
(-2.4)
P 		52.3
(-3)
P 		389
avr 487.7
(3) 		183.2
(-1.3) 		149.2
(-1.7) 		101.2
(-2.4) 		73.4
(-2.8) 		58.2
(-3) 		430
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 65
1 2 3 4 range
A 84.7
(3)
P 		28.2
(-2.4)
P 		26.5
(-2.5)
P 		21.7
(-3)
P 		63
B 71.3
(3)
P 		21
(-3)
P 		30.9
(-1.8)
P 		21.3
(-3)
P 		50
C 86
(3)
P 		25.8
(-3)
P 		41.3
(-1.5)
P 		25.8
(-3)
P 		60
avr 80.7
(3) 		25
(-2.8) 		32.9
(-2) 		22.9
(-3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 161
1 2 3 4 5 6 range
A 94.6
(3)
P 		56.5
(0.1)
P 		35.3
(-1.5)
P 		26
(-2.2)
P 		16.8
(-2.9)
P 		15.7
(-3)
P 		79
B 177.1
(3)
P 		65.6
(-1.3)
P 		35.1
(-2.5)
P 		22.4
(-3)
P 		30.4
(-2.7)
P 		21.6
(-3)
P 		156
C 163.5
(3)
P 		59.2
(-1.3)
P 		35.8
(-2.2)
P 		23.5
(-2.8)
P 		29.1
(-2.5)
P 		17.5
(-3)
P 		146
avr 145.1
(3) 		60.4
(-1) 		35.4
(-2.2) 		24
(-2.7) 		25.4
(-2.7) 		18.3
(-3) 		127
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188921_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11346
Sequence Type Exemplar sequence
Representative Public ID F25D1.5 NCBI
Target Description F25D1.5 /REP_DB=WormBase Gene ID /WP=CE05726 /TR=Q19774 /GB=CAA98264.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073303(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073303 NCBI Caenorhabditis elegans F25D1.5 (dehydrogenase) mRNA, complete cds. 11/11 None
SNAP00000015981 ENSEMBL cdna:SNAP chromosome:CEL140:V:10548410:10549333:-1 11/11 None
GENEFINDER00000015985 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10548410:10549333:-1 11/11 None
F25D1.5 ENSEMBL cdna:known chromosome:CEL140:V:10548410:10549333:-1 gene:F25D1.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10548409-10549333(-) 100.0 100.0
Public Domain and Genome References
Gene Title dehydrogenase
Ensembl F25D1.5
Entrez Gene 184922 Entrez gene
SwissProt Q19774 EMBL-EBI
WormBase CE05726 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98264 Hypothetical protein F25D1.5 [Caenorhabditis elegans] ref|NP_505704.1| F25D1.5 [Caenorhabditis elegans] 1.0E-152
blast 1XHL Chain , Crystal Structure Of Putative Tropinone Reductase-Ii From Caenorhabditis Elegans With Cofactor And Substrate pdb|1XHL|A Chain A, Crystal Structure Of Putative Tropinone Reductase-Ii From Caenorhabditis Elegans With Cofactor And Substrate 1.0E-152
blast CAE64839 Hypothetical protein CBG09635 [Caenorhabditis briggsae] 1.0E-138
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 1.6E-56
Sequence
Target Sequence 
>C. ELEGANS:188921_AT
gatatcatcaacacgactcttgctaaattcggaaaaattgatatcttggttaataatgct
ggtgccaatttagctgacggaacagctaatacggatcaaccagtggagttgtatcagaaa
acattcaagttaaacttccaagctgtgatcgaaatgactcagaaaacaaaggaacatttg
ataaagacaaaaggagagattgtaaatgtttccagtatcgttgctggcccacaagcacac
tctggatatccatattatgcttgcgcaaaagctgcgcttgaccaatacaccagatgcact
gcaatcgatcttattcaacatggtgtccgcgtaaattctgtcagccctggagcagttgcc
actggtttcatgggagcgatgggtttaccagaaactgcatctgacaagctttacagtttt
attggctcacggaaagaatgcattcctgttgggcattgtggaaagccagaagaaattgcc
aatatcattgtattcctggctgatagaaacctctcatcttacattattgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATATCATCAACACGACTCTTGCTA 108 427 235 Antisense
CTGGTGCCAATTTAGCTGACGGAAC 300 241 293 Antisense
CACAAGCACACTCTGGATATCCATA 82 195 464 Antisense
GATGCACTGCAATCGATCTTATTCA 382 413 527 Antisense
GGTGTCCGCGTAAATTCTGTCAGCC 670 499 556 Antisense
AATTCTGTCAGCCCTGGAGCAGTTG 671 175 568 Antisense
GGAGCAGTTGCCACTGGTTTCATGG 135 521 583 Antisense
GAAACTGCATCTGACAAGCTTTACA 369 357 625 Antisense
GCTTTACAGTTTTATTGGCTCACGG 261 305 642 Antisense
ATTCCTGGCTGATAGAAACCTCTCA 312 9 726 Antisense
AAACCTCTCATCTTACATTATTGGA 90 127 741 Antisense
Sequence Source Affymetrix Proprietary Database