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[188906_s_at]

Description F25E2.5 /REP_DB=WormBase Gene ID /WP=CE26364 /CHR=X /FEA=Sanger Annotation /DEF=locus:daf-3 (ST.LOUIS) TR:Q19783 protein_id:AAA91258.1
Cluster 6grp_highest_10_10 > 62      6grp_high1_10_10 > 26      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 50      6grp_total > 26      4grp_total > 47      6grp_highest_sub > Cu      6grp_cgc > 14      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 851
1 2 3 4 range
A 1200.1
(3)
P 		404.2
(-2.7)
P 		440.3
(-2.5)
P 		369.2
(-3)
P 		831
B 995.2
(3)
P 		513.9
(-1.5)
P 		349.6
(-3)
P 		630.8
(-0.4)
P 		646
C 1077.2
(3)
P 		509.4
(-2.5)
P 		496.9
(-2.6)
P 		452.5
(-3)
P 		625
avr 1090.8
(3) 		475.8
(-2.6) 		428.9
(-3) 		484.2
(-2.5) 		662

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 615
1 2 3 4 5 6 range
A 650.5
(1.7)
P 		744.4
(3)
P 		400.8
(-1.7)
P 		428.9
(-1.3)
P 		304.5
(-3)
P 		526.3
(0)
P 		440
B 897.8
(3)
P 		657
(0.7)
P 		382.3
(-2)
P 		343
(-2.4)
P 		282.9
(-3)
P 		447.1
(-1.4)
P 		615
C 872.9
(3)
P 		568.4
(-0.5)
P 		540.1
(-0.8)
P 		348.6
(-3)
P 		409.7
(-2.3)
P 		417.1
(-2.2)
P 		524
avr 807.1
(3) 		656.6
(1.1) 		441.1
(-1.6) 		373.5
(-2.5) 		332.4
(-3) 		463.5
(-1.3) 		475
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 383
1 2 3 4 range
A 437
(3)
P 		87.7
(-2.5)
P 		115.1
(-2)
P 		54
(-3)
P 		383
B 317.4
(3)
P 		126.9
(-1.8)
P 		81
(-3)
P 		134.4
(-1.6)
P 		236
C 356.6
(3)
P 		146.5
(-1.5)
P 		173.2
(-0.9)
P 		76.3
(-3)
P 		280
avr 370.3
(3) 		120.4
(-2.3) 		123.1
(-2.3) 		88.2
(-3) 		282

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 254
1 2 3 4 5 6 range
A 119.9
(-1.9)
P 		264.8
(3)
P 		87.3
(-3)
P 		108.8
(-2.3)
P 		91.9
(-2.8)
P 		128.1
(-1.6)
P 		178
B 312.4
(3)
P 		218.7
(0.5)
P 		92.8
(-2.9)
P 		90.9
(-3)
P 		114.9
(-2.3)
P 		166.7
(-0.9)
P 		222
C 323.6
(3)
P 		173.4
(-0.6)
P 		137.5
(-1.4)
P 		70.1
(-3)
P 		132.6
(-1.5)
P 		139.6
(-1.4)
P 		254
avr 252
(3) 		219
(1.8) 		105.9
(-2.4) 		89.9
(-3) 		113.1
(-2.1) 		144.8
(-1) 		162
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188906_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18306
Sequence Type Exemplar sequence
Representative Public ID F25E2.5 NCBI
Target Description F25E2.5 /REP_DB=WormBase Gene ID /WP=CE26364 /CHR=X /FEA=Sanger Annotation /DEF=locus:daf-3 (ST.LOUIS) TR:Q19783 protein_id:AAA91258.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029434(11), NM_001029433(11), NM_075760(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029434 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-3) (daf-3) mRNA, complete cds. 11/11 None
NM_001029433 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-3) (daf-3) mRNA, complete cds. 11/11 None
NM_075760 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-3) (daf-3) mRNA, complete cds. 11/11 A 
SNAP00000017578 ENSEMBL cdna:SNAP chromosome:CEL140:X:818319:823840:-1 11/11 None
GENEFINDER00000017584 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:818319:825425:-1 11/11 None
F25E2.5c ENSEMBL cdna:known chromosome:CEL140:X:817926:823840:-1 gene:F25E2.5 11/11 A 
F25E2.5b ENSEMBL cdna:known chromosome:CEL140:X:817951:825472:-1 gene:F25E2.5 11/11 None
F25E2.5a ENSEMBL cdna:known chromosome:CEL140:X:818319:825474:-1 gene:F25E2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:818318-823840(-) 100.0 100.0
Public Domain and Genome References
Ensembl F25E2.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM54189 Abnormal dauer formation protein 3, isoform c [Caenorhabditis elegans] ref|NP_001024605.1| abnormal DAuer Formation family member (daf-3) [Caenorhabditis elegans] gb|AAB61748.1| DAF-3 [Caenorhabditis elegans] 0.0
blast AAK68348 Abnormal dauer formation protein 3, isoform a [Caenorhabditis elegans] ref|NP_508161.3| abnormal DAuer Formation family member (daf-3) [Caenorhabditis elegans] 0.0
blast AAM54188 Abnormal dauer formation protein 3, isoform b [Caenorhabditis elegans] ref|NP_001024604.1| abnormal DAuer Formation family member (daf-3) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003619 EMBL-EBI Dwarfin protein, domain A 3.3E-56
Pfam IPR001132 EMBL-EBI Dwarfin protein 1.0E-123
Pfam IPR003619 EMBL-EBI Dwarfin protein, domain A 8.0E-54
Pfam IPR001132 EMBL-EBI Dwarfin protein 1.0E-123
Pfam IPR003619 EMBL-EBI Dwarfin protein, domain A 3.3E-56
Pfam IPR001132 EMBL-EBI Dwarfin protein 1.0E-123
Pfam IPR003619 EMBL-EBI Dwarfin protein, domain A 2.9E-54
Pfam IPR001132 EMBL-EBI Dwarfin protein 1.0E-123
Sequence
Target Sequence 
>C. ELEGANS:188906_S_AT
acagtctcggacttgagccaaatccaattagagaaccagtggcgtttaaagttcgtaaag
caatagtggatggaattcgcttttcctacaaaaaagacgggagtgtttggcttcaaaacc
gcatgaagtacccggtatttgtcacttctgggtatctcgacgagcaatcaggaggcctaa
agaaggataaagtgcacaaagtttacggatgtgcgtctatcaaaacgtttggcttcaacg
tttccaaacaaatcatcagagacgcgcttctttccaagcaaatggcaacaatgtacttgc
aaggaaaattgactccgatgaattatatctacgagaagaagactcaggaagagctgcgaa
gggaagcaacacgcaccactgattcattggccaagtactgttgtgtccgtgtctcgttct
gcaaaggatttggagaagcatacccagaacgcccgtcaattcatgattgtccagtttgga
ttgagttgaaaatcaacattgcctacgatttcatggattcaatctgccagtacataacca
actgcttcgagccgctaggaatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAGTCTCGGACTTGAGCCAAATCC 108 109 1799 Antisense
GTGGATGGAATTCGCTTTTCCTACA 666 489 1864 Antisense
GGGAGTGTTTGGCTTCAAAACCGCA 215 491 1897 Antisense
AAGTACCCGGTATTTGTCACTTCTG 579 155 1924 Antisense
TGTCACTTCTGGGTATCTCGACGAG 458 559 1938 Antisense
AAATCATCAGAGACGCGCTTCTTTC 646 117 2048 Antisense
AACACGCACCACTGATTCATTGGCC 247 135 2166 Antisense
TGGCCAAGTACTGTTGTGTCCGTGT 293 543 2186 Antisense
GTCCGTGTCTCGTTCTGCAAAGGAT 66 477 2203 Antisense
CCCAGAACGCCCGTCAATTCATGAT 73 259 2241 Antisense
CAACTGCTTCGAGCCGCTAGGAATG 680 187 2337 Antisense
Sequence Source Affymetrix Proprietary Database