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[188886_at]

Description DC2.7 /REP_DB=WormBase Gene ID /WP=CE20636 /CHR=5 /FEA=Sanger Annotation /DEF=protein kinase (ST.LOUIS) TR:Q9UAW6 protein_id:AAD14728.1
Cluster 6grp_total > 33      4grp_total > 05      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 23.6
(-0.8)
A 		20.8
(-1.2)
A 		8.4
(-3)
A 		49.1
(3)
A 		41
B 50.1
(3)
A 		7.7
(-2.7)
A 		5.1
(-3)
A 		10.7
(-2.3)
A 		45
C 6.9
(-3)
A 		11
(-2.7)
A 		15.4
(-2.3)
A 		80.4
(3)
A 		74
avr 26.9
(-0.2) 		13.2
(-2.4) 		9.6
(-3) 		46.7
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 96
1 2 3 4 5 6 range
A 102.7
(3)
A 		52.9
(-0.2)
A 		69.3
(0.8)
A 		10.7
(-2.9)
A 		16.2
(-2.6)
A 		9.8
(-3)
A 		93
B 23.6
(-0.5)
A 		39.6
(2.4)
A 		17.3
(-1.6)
A 		9.9
(-3)
A 		42.7
(3)
A 		22.8
(-0.6)
A 		33
C 12.2
(-2.3)
A 		26
(-0.5)
A 		46.4
(2.2)
A 		52.1
(3)
A 		7.6
(-2.9)
A 		7.2
(-3)
A 		45
avr 46.2
(3) 		39.5
(1.8) 		44.3
(2.7) 		24.2
(-1) 		22.2
(-1.4) 		13.3
(-3) 		33
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 8.6
(3)
A 		4.5
(-0.8)
A 		2.2
(-3)
A 		7.2
(1.7)
A 		6
B 16
(3)
A 		1.9
(-2.7)
A 		1.2
(-3)
A 		2.3
(-2.6)
A 		15
C 2.3
(-3)
A 		3.2
(-2.5)
A 		5.4
(-1.4)
A 		13.6
(3)
A 		11
avr 9
(3) 		3.2
(-2.7) 		2.9
(-3) 		7.7
(1.7) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 18.9
(3)
A 		18.8
(3)
A 		15.1
(1.6)
A 		2.7
(-2.9)
A 		4.9
(-2.1)
A 		2.4
(-3)
A 		17
B 8.2
(-0.7)
A 		13.2
(1.3)
A 		4.2
(-2.4)
A 		2.6
(-3)
A 		17.4
(3)
A 		8.5
(-0.6)
A 		15
C 4.5
(-1.7)
A 		7.9
(0.5)
A 		11.8
(3)
A 		10.5
(2.2)
A 		2.5
(-2.9)
A 		2.4
(-3)
A 		9
avr 10.5
(1.1) 		13.3
(3) 		10.4
(1) 		5.3
(-2.4) 		8.3
(-0.4) 		4.4
(-3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188886_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15844
Sequence Type Exemplar sequence
Representative Public ID DC2.7 NCBI
Target Description DC2.7 /REP_DB=WormBase Gene ID /WP=CE20636 /CHR=5 /FEA=Sanger Annotation /DEF=protein kinase (ST.LOUIS) TR:Q9UAW6 protein_id:AAD14728.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028589(9), NM_070758(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028589 NCBI Caenorhabditis elegans DC2.7c (DC2.7) mRNA, complete cds. 9/11 None
NM_070758 NCBI Caenorhabditis elegans DC2.7a (DC2.7) mRNA, complete cds. 9/11 A 
DC2.7a ENSEMBL cdna:known chromosome:CEL140:V:228760:234497:-1 gene:DC2.7 9/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000026554 7/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
178549 3 2 NM_001028589 188886_at (9), 188887_s_at (11)
NM_070758 188886_at (9), 188887_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:227271-234497(-) 100.0 100.0
Public Domain and Genome References
Ensembl DC2.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001023760 DC2.7c [Caenorhabditis elegans] gb|AAX55694.1| Hypothetical protein DC2.7c [Caenorhabditis elegans] 0.0
blast NP_503159 DC2.7a [Caenorhabditis elegans] gb|AAD14728.2| Hypothetical protein DC2.7a [Caenorhabditis elegans] 0.0
blast NP_503160 DC2.7b [Caenorhabditis elegans] gb|AAK68289.1| Hypothetical protein DC2.7b [Caenorhabditis elegans] 1.0E-173
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-59
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-62
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-37
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 5.0E-37
hanks 2.2.5 CaMK Group; CaMK II KIN1/SNF1/Nim1; SNF1 3.0E-62
hanks 1.4.7 AGC group; AGC IV GPCR kinases; DmGPRK1 4.0E-59
hanks 1.4.2 AGC group; AGC IV GPCR kinases; bARK2 4.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 2.2E-59
Pfam IPR000719 EMBL-EBI Protein kinase 5.5E-70
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-70
Sequence
Target Sequence 
>C. ELEGANS:188886_AT
gaatcgcttatggacacccgatgtgggacacctcaatacgctgcacctgaagttttggtt
ggaaatcaataccgtggaccgcccgtagacatttggtcagctggagttgtgctcattaat
atgttgactgggcatagcccatggaaaaaggcgtgcaactttaaaacacagtacttttca
attttaattttgaattttaaatttgcttggatttttcaaatttctcaaattaaatcaatg
caatccgatgcctcctacactcgctggttcaatgacgaatgcaccgaaagagatgcgtgg
tctgatcttggtgctcgtgtaatcacacttctttgctcaattcttgctcacaacccaaca
catcgagcgccaattgagctcattgaacaggaccactggtttcagtttggtgatgaggaa
actgttgaaatcacttccgcttcatcaaccagttgcaactccgaagccaaatccagcaga
aaacggaaaattgagcagactattgaatcccgtcagatcttgatggacacggacttttgt
tggatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCGCTTATGGACACCCGATGTG 325 329 1477 Antisense
ACCCGATGTGGGACACCTCAATACG 605 89 1492 Antisense
CCTCAATACGCTGCACCTGAAGTTT 279 269 1507 Antisense
TGGAAATCAATACCGTGGACCGCCC 669 547 1536 Antisense
TGGACCGCCCGTAGACATTTGGTCA 223 549 1551 Antisense
TAGACATTTGGTCAGCTGGAGTTGT 660 651 1562 Antisense
TAATATGTTGACTGGGCATAGCCCA 425 635 1593 Antisense
AAGGCGTGCAACTTTAAAACACAGT 193 157 1624 Antisense
AATCAATGCAATCCGATGCCTCCTA 472 171 1709 Antisense
ATTGAATCCCGTCAGATCTTGATGG 357 5 1978 Antisense
GATGGACACGGACTTTTGTTGGATT 513 407 1998 Antisense
Sequence Source Affymetrix Proprietary Database