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[188882_at] dehydrogenase

Description R08H2.1 /REP_DB=WormBase Gene ID /WP=CE12594 /TR=O45709 /GB=CAB04628.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=dehydrogenase
Cluster 6grp_total > 08      4grp_total > 54      6grp_cgc > 11      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 222
1 2 3 4 range
A 256.9
(3)
P 		158.7
(0.2)
A 		136.7
(-0.5)
A 		49.2
(-3)
A 		208
B 212
(3)
P 		94.1
(-1)
A 		110.5
(-0.4)
A 		34.6
(-3)
A 		177
C 204
(3)
P 		134
(0.3)
A 		110.1
(-0.6)
M 		47.8
(-3)
A 		156
avr 224.3
(3) 		128.9
(-0.2) 		119.1
(-0.5) 		43.9
(-3) 		180

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 157
1 2 3 4 5 6 range
A 145.2
(3)
P 		53.8
(-0.9)
A 		26.1
(-2)
A 		42.1
(-1.3)
A 		20.3
(-2.3)
A 		2.8
(-3)
A 		142
B 159.3
(3)
A 		64.6
(-0.6)
A 		45.4
(-1.3)
A 		42.2
(-1.5)
A 		38.4
(-1.6)
A 		1.9
(-3)
A 		157
C 154.3
(3)
P 		48.2
(-1.2)
A 		61.2
(-0.7)
A 		3.3
(-3)
A 		37.1
(-1.7)
A 		42.4
(-1.4)
A 		151
avr 152.9
(3) 		55.5
(-1.3) 		44.2
(-1.8) 		29.2
(-2.4) 		31.9
(-2.3) 		15.7
(-3) 		137
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 93.5
(3)
P 		34.4
(-1.1)
A 		35.7
(-1)
A 		7.2
(-3)
A 		86
B 67.6
(3)
P 		23.2
(-1.4)
A 		25.6
(-1.2)
A 		7.4
(-3)
A 		60
C 67.5
(3)
P 		38.5
(0.1)
A 		38.4
(0.1)
M 		8.1
(-3)
A 		59
avr 76.2
(3) 		32
(-0.9) 		33.2
(-0.8) 		7.6
(-3) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 57
1 2 3 4 5 6 range
A 26.8
(3)
P 		19.1
(1.2)
A 		5.7
(-1.9)
A 		10.7
(-0.7)
A 		6.1
(-1.8)
A 		0.7
(-3)
A 		26
B 55.4
(3)
A 		21.5
(-0.7)
A 		11
(-1.9)
A 		11.2
(-1.8)
A 		15.6
(-1.4)
A 		0.7
(-3)
A 		55
C 57.2
(3)
P 		14.7
(-1.5)
A 		15.6
(-1.4)
A 		0.7
(-3)
A 		12
(-1.8)
A 		14.2
(-1.6)
A 		57
avr 46.5
(3) 		18.4
(-1.1) 		10.8
(-2.2) 		7.5
(-2.7) 		11.2
(-2.1) 		5.2
(-3) 		41
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188882_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13945
Sequence Type Exemplar sequence
Representative Public ID R08H2.1 NCBI
Target Description R08H2.1 /REP_DB=WormBase Gene ID /WP=CE12594 /TR=O45709 /GB=CAB04628.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074419(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074419 NCBI Caenorhabditis elegans R08H2.1 (dehydrogenase) mRNA, complete cds. 11/11 None
SNAP00000012525 ENSEMBL cdna:SNAP chromosome:CEL140:V:15383847:15384775:1 11/11 None
GENEFINDER00000012531 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15383847:15384775:1 11/11 None
R08H2.1 ENSEMBL cdna:known chromosome:CEL140:V:15383683:15384775:1 gene:R08H2.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15383846-15384775(+) 100.0 100.0
Public Domain and Genome References
Gene Title dehydrogenase
Gene Symbol dhs-23
Ensembl R08H2.1
Entrez Gene 180038 Entrez gene
SwissProt O45709 EMBL-EBI
WormBase CE12594 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:258814_AT short-chain dehydrogenase/reductase (SDR) family protein at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04628 Hypothetical protein R08H2.1 [Caenorhabditis elegans] ref|NP_506820.1| R08H2.1 [Caenorhabditis elegans] 1.0E-141
blast CAA98264 Hypothetical protein F25D1.5 [Caenorhabditis elegans] ref|NP_505704.1| F25D1.5 [Caenorhabditis elegans] 1.0E-127
blast 1XHL Chain , Crystal Structure Of Putative Tropinone Reductase-Ii From Caenorhabditis Elegans With Cofactor And Substrate pdb|1XHL|A Chain A, Crystal Structure Of Putative Tropinone Reductase-Ii From Caenorhabditis Elegans With Cofactor And Substrate 1.0E-127
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 6.9E-57
Sequence
Target Sequence 
>C. ELEGANS:188882_AT
tgctggtgctcttttgactgatggaacatctaatacggatcaagcagtagagttgtatca
gaaaacattcaaattaaacttccaagctgtagtcgaaatgactcagaaaaccaaggagta
tttgatcaagacaaaaggagaaattgtaaatgtttccagtatagttgctggtccacaagc
acaacctgcttccccatactacgcatgtgcaaaagctgcgctagaccaatacaccagatg
tactgcaattgacctcattcaacacggtgtccgcgtaaactcggttagccctggagtaat
tgccaccggtttcttgggagcaatgggattgcctgagactgcatcggacaagttttataa
tttcatgggctcgcaaaaggaatgcattcctgtcggacattgtggaaagccggaagaaat
tgcgaatattattgtattcctcgcagatcggcgtctctcgtcgtacattattgggca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTGGTGCTCTTTTGACTGATGGA 336 583 291 Antisense
AGTTGCTGGTCCACAAGCACAACCT 363 63 453 Antisense
TTCCCCATACTACGCATGTGCAAAA 306 699 480 Antisense
ATTGACCTCATTCAACACGGTGTCC 405 5 538 Antisense
TGTCCGCGTAAACTCGGTTAGCCCT 188 557 558 Antisense
TAGCCCTGGAGTAATTGCCACCGGT 70 649 576 Antisense
TGCCACCGGTTTCTTGGGAGCAATG 596 581 591 Antisense
GGATTGCCTGAGACTGCATCGGACA 642 507 616 Antisense
GGAATGCATTCCTGTCGGACATTGT 411 531 669 Antisense
ATTGTATTCCTCGCAGATCGGCGTC 355 5 721 Antisense
GTCTCTCGTCGTACATTATTGGGCA 553 469 743 Antisense
Sequence Source Affymetrix Proprietary Database