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[188855_at] 7TM receptor

Description T21B4.5 /REP_DB=WormBase Gene ID /WP=CE16430 /TR=O18101 /GB=CAB03373.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=7TM receptor
Cluster 6grp_total > 08      4grp_total > 11      6grp_cgc > 0      6grp_total_sub > Decrease      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 15
(-1.4)
A 		34.5
(1.4)
A 		4.1
(-3)
A 		45.5
(3)
A 		41
B 20
(-0.4)
A 		2.4
(-3)
A 		8.6
(-2.1)
A 		43.3
(3)
A 		41
C 9.3
(-2.4)
A 		7.1
(-3)
A 		21.1
(0.8)
A 		29.4
(3)
A 		22
avr 14.8
(-2.3) 		14.7
(-2.3) 		11.3
(-3) 		39.4
(3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 60
1 2 3 4 5 6 range
A 42.8
(0.3)
A 		18.5
(-2.9)
A 		39.2
(-0.2)
P 		31.7
(-1.2)
A 		18
(-3)
A 		63.2
(3)
A 		45
B 44.8
(3)
A 		25.8
(0.3)
A 		2.9
(-3)
A 		33.8
(1.4)
A 		12.2
(-1.7)
A 		19
(-0.7)
A 		42
C 41.8
(3)
A 		29.7
(0.9)
A 		22.4
(-0.3)
A 		36.6
(2.1)
A 		25.7
(0.3)
A 		6.6
(-3)
A 		35
avr 43.1
(3) 		24.7
(-1.5) 		21.5
(-2.3) 		34
(0.8) 		18.6
(-3) 		29.6
(-0.3) 		25
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 5.5
(1.1)
A 		7.5
(3)
A 		1.1
(-3)
A 		6.7
(2.3)
A 		6
B 6.4
(1)
A 		0.6
(-3)
A 		2
(-2)
A 		9.2
(3)
A 		9
C 3.1
(-1.8)
A 		2
(-3)
A 		7.4
(3)
A 		5
(0.3)
A 		5
avr 5
(-0.3) 		3.4
(-3) 		3.5
(-2.8) 		7
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 7.9
(-1.5)
A 		6.6
(-2.3)
A 		8.5
(-1.1)
P 		8.1
(-1.4)
A 		5.4
(-3)
A 		15.4
(3)
A 		10
B 15.6
(3)
A 		8.6
(0.2)
A 		0.7
(-3)
A 		8.9
(0.3)
A 		5
(-1.3)
A 		7.1
(-0.4)
A 		15
C 15.5
(3)
A 		9.1
(0.1)
A 		5.7
(-1.4)
A 		7.4
(-0.7)
A 		8.3
(-0.2)
A 		2.2
(-3)
A 		13
avr 13
(3) 		8.1
(-0.7) 		5
(-3) 		8.1
(-0.7) 		6.2
(-2.1) 		8.2
(-0.6) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188855_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2607
Sequence Type Exemplar sequence
Representative Public ID T21B4.5 NCBI
Target Description T21B4.5 /REP_DB=WormBase Gene ID /WP=CE16430 /TR=O18101 /GB=CAB03373.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=7TM receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064267(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064267 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-68) (srh-68) mRNA, complete cds. 11/11 None
SNAP00000006688 ENSEMBL cdna:SNAP chromosome:CEL140:II:12507768:12510038:1 9/11 None
GENEFINDER00000006699 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:12507241:12508355:1 11/11 None
T21B4.5 ENSEMBL cdna:known chromosome:CEL140:II:12507241:12508355:1 gene:T21B4.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:12507237-12508352(+) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM receptor
Gene Symbol srh-68
Ensembl T21B4.5
Entrez Gene 188674 Entrez gene
SwissProt O18101 EMBL-EBI
WormBase CE16430 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03373 Hypothetical protein T21B4.5 [Caenorhabditis elegans] ref|NP_496668.1| Serpentine Receptor, class H family member (srh-68) [Caenorhabditis elegans] 1.0E-179
blast CAB03373 Hypothetical protein T21B4.5 [Caenorhabditis elegans] ref|NP_496668.1| Serpentine Receptor, class H family member (srh-68) [Caenorhabditis elegans] 1.0E-166
blast CAB03372 Hypothetical protein T21B4.6 [Caenorhabditis elegans] ref|NP_496669.1| Serpentine Receptor, class H family member (srh-67) [Caenorhabditis elegans] 1.0E-144
blast CAB03372 Hypothetical protein T21B4.6 [Caenorhabditis elegans] ref|NP_496669.1| Serpentine Receptor, class H family member (srh-67) [Caenorhabditis elegans] 1.0E-131
blast T25056 hypothetical protein T21B4.10 - Caenorhabditis elegans 1.0E-112
blast T25056 hypothetical protein T21B4.10 - Caenorhabditis elegans 1.0E-109
blast CAB03373 Hypothetical protein T21B4.5 [Caenorhabditis elegans] ref|NP_496668.1| Serpentine Receptor, class H family member (srh-68) [Caenorhabditis elegans] 9.0E-86
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 3.9E-67
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 3.9E-54
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.5E-123
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496668.1 7 29-51,70-92,107-129,150-172,216-238,255-277,287-309
T21B4.5 7 29-51,70-92,107-129,150-172,216-238,255-277,287-309
SNAP00000006688 6 56-78,93-115,127-149,206-228,245-267,277-299
GENEFINDER00000006699 6 54-76,91-113,134-156,200-222,239-261,271-293
Sequence
Target Sequence 
>C. ELEGANS:188855_AT
gttcgcattaacgggacacggcatcgaatttattgggtttccgcaaactttttcacaata
atgtttctcttaactgtaacatttttgaatctccctgatcagaatcaagcgaaaatcgat
atattgaagactcttccctgtcccaccaaggagttcttcactgagcccttcattatgctc
gccgcccctggactctgggaagactacatggttaccaccacttcattattgaatatggga
tttatatttcaagtttccctcttctccagctgctgtatttattacctattcatcgataaa
agctcatttgcatcgccggaaacccaaaaagctcaggctcgatcgtttattggaattgtc
ctgcaaaccttcttgcctatacttttagtggttttggctttggtgacaattttgaagaag
aacggaggctatgatcaagttgccaataatcttatgtttattttcatggattttcatagt
ggcgttgcgagcttgtcgattcttttggtgcaacatccttatcggaagtttttgatctcg
tttttgtgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCGCATTAACGGGACACGGCATC 126 441 433 Antisense
GGCATCGAATTTATTGGGTTTCCGC 209 507 452 Antisense
GTAACATTTTTGAATCTCCCTGATC 701 461 508 Antisense
TCTTCACTGAGCCCTTCATTATGCT 74 615 587 Antisense
CGCCCCTGGACTCTGGGAAGACTAC 506 235 615 Antisense
GGGAAGACTACATGGTTACCACCAC 391 335 629 Antisense
TGGTTACCACCACTTCATTATTGAA 346 551 641 Antisense
CCTCTTCTCCAGCTGCTGTATTTAT 692 267 690 Antisense
GGAGGCTATGATCAAGTTGCCAATA 518 515 856 Antisense
CATAGTGGCGTTGCGAGCTTGTCGA 532 213 907 Antisense
GAAGTTTTTGATCTCGTTTTTGTGC 7 335 957 Antisense
Sequence Source Affymetrix Proprietary Database