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[188851_at] COA ligase

Description ZK1127.2 /REP_DB=WormBase Gene ID /WP=CE07642 /TR=Q23404 /GB=AAB93427.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=COA ligase
Cluster 6grp_total > 87      4grp_total > 20      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 71
1 2 3 4 range
A 29
(3)
A 		12.2
(-2.7)
A 		26.7
(2.2)
A 		11.4
(-3)
A 		18
B 43.7
(0.2)
A 		19.3
(-1.8)
A 		5.7
(-3)
A 		76.5
(3)
A 		71
C 17.2
(-3)
A 		43.4
(3)
A 		21.1
(-2.1)
A 		40
(2.2)
A 		26
avr 30
(-0) 		25
(-1.3) 		17.8
(-3) 		42.6
(3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 26
(-3)
A 		34.4
(-2.4)
A 		47.5
(-1.5)
A 		51.3
(-1.2)
A 		54.2
(-1)
A 		110.9
(3)
M 		85
B 22
(-3)
A 		23.5
(-2.9)
A 		22.6
(-3)
A 		27.8
(-2.6)
A 		54.8
(-0.7)
A 		108.1
(3)
P 		86
C 19
(-3)
A 		26.1
(-2.4)
A 		25.3
(-2.5)
A 		53.4
(-0.1)
A 		50.8
(-0.3)
M 		90.2
(3)
P 		71
avr 22.3
(-3) 		28
(-2.6) 		31.8
(-2.3) 		44.2
(-1.4) 		53.3
(-0.7) 		103.1
(3) 		81
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 10.6
(3)
A 		2.7
(-2.3)
A 		7
(0.6)
A 		1.7
(-3)
A 		9
B 13.9
(2)
A 		4.8
(-1.6)
A 		1.3
(-3)
A 		16.3
(3)
A 		15
C 5.7
(-3)
A 		12.5
(3)
A 		7.4
(-1.5)
A 		6.7
(-2.1)
A 		7
avr 10.1
(3) 		6.7
(-1.2) 		5.2
(-3) 		8.2
(0.7) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 4.8
(-3)
A 		12.2
(-1)
A 		10.4
(-1.5)
A 		13
(-0.8)
A 		16.4
(0.1)
A 		27
(3)
M 		22
B 7.7
(-2.6)
A 		7.8
(-2.6)
A 		5.5
(-3)
A 		7.4
(-2.7)
A 		22.3
(-0.1)
A 		40.3
(3)
P 		35
C 7
(-2.8)
A 		8
(-2.6)
A 		6.4
(-3)
A 		10.7
(-1.9)
A 		16.4
(-0.5)
M 		30.2
(3)
P 		24
avr 6.5
(-3) 		9.3
(-2.4) 		7.4
(-2.8) 		10.4
(-2.1) 		18.4
(-0.3) 		32.5
(3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188851_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4192
Sequence Type Exemplar sequence
Representative Public ID ZK1127.2 NCBI
Target Description ZK1127.2 /REP_DB=WormBase Gene ID /WP=CE07642 /TR=Q23404 /GB=AAB93427.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=COA ligase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063049(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063049 NCBI Caenorhabditis elegans ZK1127.2 (ZK1127.2) mRNA, complete cds. 11/11 None
SNAP00000033595 ENSEMBL cdna:SNAP chromosome:CEL140:II:7062550:7064651:1 11/11 None
GENEFINDER00000033609 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7062550:7064651:1 11/11 None
ZK1127.2 ENSEMBL cdna:known chromosome:CEL140:II:7061932:7064697:1 gene:ZK1127.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7062545-7064647(+) 100.0 100.0
Public Domain and Genome References
Gene Title COA ligase
Ensembl ZK1127.2
Entrez Gene 174156 Entrez gene
SwissProt Q23404 EMBL-EBI
WormBase CE07642 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB93427 Hypothetical protein ZK1127.2 [Caenorhabditis elegans] ref|NP_495450.1| ZK1127.2 [Caenorhabditis elegans] 0.0
blast CAB02686 Hypothetical protein AH10.1 [Caenorhabditis elegans] ref|NP_506502.1| AH10.1 [Caenorhabditis elegans] 0.0
blast CAE71795 Hypothetical protein CBG18805 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 1.0E-153
Protein Domains
Database ID Description E-Value
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 7.7E-91
Sequence
Target Sequence 
>C. ELEGANS:188851_AT
gaaactgttggaaacttggcttcaacttatgaattgaaaatcttggacaaggagaaaaaa
gagattaccacaatcaacacggttggtcaattgtgtttccgtggaccaacagtcatgaag
ggatacttgaagagagaagaagctgatatcattgataaggatggtttcttgttgactgga
gatctgggatctattgatgacaagggacgtattcatgtgactggacgaatcaaggagttg
atcaaggttaatggaatgcaggttccaccggtcgagattgaggatgttcttcttcttcat
ccaaaagttaaggattgtgctgtaattggtgttcctgatgagcacaaaggagaatctcca
aaagcgtatattgtgaagaaggaccacacactgactgaagcagagctcactgagtttgtt
cgtcaaaagttgtcatcttacaaatggattgatacatatgaattcattgattcgattcca
aagcttccatccggaaaaatccaacgcaagaaattgaagaaaatggcagagtcgtcggga
tccacagaatcggaagcatcggagccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAACTGTTGGAAACTTGGCTTCAA 629 355 1105 Antisense
GGTTGGTCAATTGTGTTTCCGTGGA 517 505 1185 Antisense
GTTTCCGTGGACCAACAGTCATGAA 193 449 1199 Antisense
GATGGTTTCTTGTTGACTGGAGATC 663 407 1264 Antisense
AGGTTCCACCGGTCGAGATTGAGGA 357 57 1364 Antisense
GATTGTGCTGTAATTGGTGTTCCTG 528 435 1417 Antisense
AGAAGGACCACACACTGACTGAAGC 209 59 1481 Antisense
AGCTCACTGAGTTTGTTCGTCAAAA 703 77 1508 Antisense
TGATTCGATTCCAAAGCTTCCATCC 44 565 1572 Antisense
AATGGCAGAGTCGTCGGGATCCACA 690 159 1626 Antisense
ACAGAATCGGAAGCATCGGAGCCAA 182 109 1648 Antisense
Sequence Source Affymetrix Proprietary Database