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[188837_at] phosphatase

Description ZK973.3 /REP_DB=WormBase Gene ID /WP=CE24749 /TR=Q9N4M0 /GB=AAF40007.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=phosphatase
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 85      4grp_highest_8_8 > 33      4grp_high_8_8 > 50      6grp_highest_nonorm > 96      6grp_total > 76      4grp_total > 61      6grp_highest_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 518
1 2 3 4 range
A 791.1
(2.2)
P 		838.4
(3)
P 		741.8
(1.5)
P 		462.3
(-3)
P 		376
B 901.6
(3)
P 		719.8
(0)
P 		783.4
(1.1)
P 		534.5
(-3)
P 		367
C 968
(3)
P 		663.1
(-0.5)
P 		742.8
(0.4)
P 		449.6
(-3)
P 		518
avr 886.9
(3) 		740.4
(0.8) 		756
(1.1) 		482.1
(-3) 		405

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 631
1 2 3 4 5 6 range
A 758.7
(-2)
P 		694.2
(-3)
P 		771.6
(-1.8)
P 		771.2
(-1.8)
P 		853.1
(-0.5)
P 		1074
(3)
P 		380
B 688.5
(-3)
P 		730.8
(-2.5)
P 		931
(0.1)
P 		885.5
(-0.5)
P 		912
(-0.2)
P 		1164.3
(3)
P 		476
C 533
(-3)
P 		734.8
(-1.1)
P 		838.8
(-0.1)
P 		848
(0)
P 		779.1
(-0.6)
P 		1157.8
(3)
P 		625
avr 660.1
(-3) 		719.9
(-2.2) 		847.1
(-0.6) 		834.9
(-0.8) 		848.1
(-0.6) 		1132
(3) 		472
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 253
1 2 3 4 range
A 288
(3)
P 		182
(0.1)
P 		193.9
(0.4)
P 		67.7
(-3)
P 		220
B 287.5
(3)
P 		177.8
(-0.8)
P 		181.5
(-0.7)
P 		113.9
(-3)
P 		174
C 320.4
(3)
P 		190.6
(-0.2)
P 		258.9
(1.5)
P 		75.8
(-3)
P 		245
avr 298.6
(3) 		183.5
(-0.2) 		211.4
(0.5) 		85.8
(-3) 		213

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 294
1 2 3 4 5 6 range
A 139.9
(-3)
P 		246.9
(2.3)
P 		168
(-1.6)
P 		195.6
(-0.3)
P 		257.4
(2.8)
P 		261.5
(3)
P 		122
B 239.6
(-2.6)
P 		243.2
(-2.5)
P 		226.1
(-3)
P 		234.5
(-2.8)
P 		370.5
(1.2)
P 		434.2
(3)
P 		208
C 197.6
(-2.3)
P 		224.2
(-1.5)
P 		213.5
(-1.8)
P 		170.5
(-3)
P 		252.1
(-0.7)
P 		387.6
(3)
P 		217
avr 192.4
(-3) 		238.1
(-1.4) 		202.5
(-2.6) 		200.2
(-2.7) 		293.3
(0.6) 		361.1
(3) 		169
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188837_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.374
Sequence Type Exemplar sequence
Representative Public ID ZK973.3 NCBI
Target Description ZK973.3 /REP_DB=WormBase Gene ID /WP=CE24749 /TR=Q9N4M0 /GB=AAF40007.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=phosphatase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058956(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058956 NCBI Caenorhabditis elegans ZK973.3 (phosphatase) mRNA, complete cds. 11/11 None
SNAP00000023510 ENSEMBL cdna:SNAP chromosome:CEL140:I:4368107:4370274:1 11/11 None
GENEFINDER00000023518 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4368107:4370274:1 11/11 None
ZK973.3.1 ENSEMBL cdna:known chromosome:CEL140:I:4368088:4370481:1 gene:ZK973.3 11/11 None
ZK973.3.2 ENSEMBL cdna:known chromosome:CEL140:I:4368090:4370481:1 gene:ZK973.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4368106-4370274(+) 100.0 100.0
Public Domain and Genome References
Gene Title phosphatase
Ensembl ZK973.3
Entrez Gene 172035 Entrez gene
SwissProt Q9N4M0 EMBL-EBI
WormBase CE24749 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1586099_AT similar to pyruvate dehydrogenase phosphatase precursor cfa
CANINE_2:CFA.11839.1.A1_AT similar to pyruvate dehydrogenase phosphatase precursor cfa
CANINE_2:CFA.11839.1.A1_S_AT similar to pyruvate dehydrogenase phosphatase precursor cfa
CANINE_2:CFAAFFX.14411.1.S1_AT similar to pyruvate dehydrogenase phosphatase precursor cfa
DROSOPHILA_2:1629629_AT dm
DROSGENOME1:153332_AT dm
CHICKEN:GGAAFFX.10158.1.S1_AT similar to pyruvate dehydrogenase phosphatase gga
HG-U133_PLUS_2:218273_S_AT protein phosphatase 2C, magnesium-dependent, catalytic subunit hs
HG-U133A_2:218273_S_AT protein phosphatase 2C, magnesium-dependent, catalytic subunit hs
HG-U133A:218273_S_AT protein phosphatase 2C, magnesium-dependent, catalytic subunit hs
HG-FOCUS:218273_S_AT protein phosphatase 2C, magnesium-dependent, catalytic subunit hs
HU35KSUBA:RC_AA398346_AT protein phosphatase 2C, magnesium-dependent, catalytic subunit hs
HU35KSUBB:RC_Z38909_AT protein phosphatase 2C, magnesium-dependent, catalytic subunit hs
U133_X3P:G8923959_3P_A_AT protein phosphatase 2C, magnesium-dependent, catalytic subunit hs
U133_X3P:HS.22265.0.S2_3P_AT protein phosphatase 2C, magnesium-dependent, catalytic subunit hs
HG-U133_PLUS_2:222572_AT protein phosphatase 2C, magnesium-dependent, catalytic subunit hs
HG-U133B:222572_AT protein phosphatase 2C, magnesium-dependent, catalytic subunit hs
HG-U95C:60812_AT protein phosphatase 2C, magnesium-dependent, catalytic subunit hs
HG-U95C:60828_AT protein phosphatase 2C, magnesium-dependent, catalytic subunit hs
HG-U95D:78594_AT protein phosphatase 2C, magnesium-dependent, catalytic subunit hs
HG-U95D:88290_AT protein phosphatase 2C, magnesium-dependent, catalytic subunit hs
MG-U74BV2:114093_AT protein phosphatase 2C, magnesium dependent, catalytic subunit mm
MG-U74CV2:171057_R_AT protein phosphatase 2C, magnesium dependent, catalytic subunit mm
MOE430B:1434228_AT protein phosphatase 2C, magnesium dependent, catalytic subunit mm
MOUSE430_2:1434228_AT protein phosphatase 2C, magnesium dependent, catalytic subunit mm
MG-U74BV2:108331_AT PREDICTED: similar to PPM2C protein [Mus musculus], mRNA sequence mm
RG-U34A:AF062740_G_AT protein phosphatase 2C, magnesium dependent, catalytic subunit rn
RG-U34A:AF062740_AT protein phosphatase 2C, magnesium dependent, catalytic subunit rn
RG-U34B:RC_AI058387_I_AT protein phosphatase 2C, magnesium dependent, catalytic subunit rn
RAE230A:1368910_AT protein phosphatase 2C, magnesium dependent, catalytic subunit rn
RAT230_2:1368910_AT protein phosphatase 2C, magnesium dependent, catalytic subunit rn
YEAST_2:1770697_AT Mitochondrially localized type 2C protein phosphatase; contains Mg2+/Mn2+-dependent casein phosphatase activity in vitro but in vivo substrates are unknown Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6470 protein amino acid dephosphorylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
8287 protein serine/threonine phosphatase complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
4722 protein serine/threonine phosphatase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF40007 Hypothetical protein ZK973.3 [Caenorhabditis elegans] ref|NP_491357.1| ZK973.3 [Caenorhabditis elegans] 0.0
blast CAE66824 Hypothetical protein CBG12191 [Caenorhabditis briggsae] 0.0
blast CAE56283 Hypothetical protein CBG23932 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001932 EMBL-EBI Protein phosphatase 2C-like 0.45
Pfam IPR001932 EMBL-EBI Protein phosphatase 2C-like 2.2E-33
Sequence
Target Sequence 
>C. ELEGANS:188837_AT
gaatcgcacatcctgcatcagaatctcaaactgttttacgaggcggtcgattattgggag
aactttttccgcttcgagcatttggagatgttcggtataaatggccactggatcttcaga
aggttgtcctcgaacccctaggtcatccacctccccaacatctcttcactccaccttatc
tatcaacatctcccgaagttttttatcacaaattaacgccaaatgataggttcctggtgt
tagccactgatggattatgggagtggctggatcctgatacagtagttcgtttagtacatg
atcacaccctcggaacgattacacagcaaccatacgtgccaaagtctggaacatcacttc
gacaagttcgtgaacagctgaaagatcgagcaagaggtgaacagaagacgaagaagccga
tcgatgagaactgtgcgacacacatcattcgtcatgcgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCGCACATCCTGCATCAGAATC 650 329 749 Antisense
ACTTTTTCCGCTTCGAGCATTTGGA 149 103 810 Antisense
TTCAGAAGGTTGTCCTCGAACCCCT 338 683 863 Antisense
CCTTATCTATCAACATCTCCCGAAG 705 267 922 Antisense
GATAGGTTCCTGGTGTTAGCCACTG 442 425 973 Antisense
GGGAGTGGCTGGATCCTGATACAGT 605 495 1007 Antisense
CGTTTAGTACATGATCACACCCTCG 225 247 1036 Antisense
CACACCCTCGGAACGATTACACAGC 114 195 1051 Antisense
ATTACACAGCAACCATACGTGCCAA 314 13 1066 Antisense
GTCTGGAACATCACTTCGACAAGTT 42 473 1092 Antisense
GCGACACACATCATTCGTCATGCGT 295 289 1183 Antisense
Sequence Source Affymetrix Proprietary Database