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[188816_s_at] chimaerin

Description C38D4.5 /REP_DB=WormBase Gene ID /WP=CE00918 /TR=SW:P46941 /GB=CAA86318.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=chimaerin
Cluster 6grp_highest_10_10 > 81      6grp_high1_10_10 > 29      4grp_highest_8_8 > 53      4grp_high_8_8 > 08      6grp_highest_nonorm > 50      6grp_total > 38      4grp_total > 31      6grp_highest_sub > Cu      6grp_cgc > 04      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 260
1 2 3 4 range
A 489.6
(2.4)
P 		401.4
(-0.8)
P 		338.9
(-3)
P 		507.2
(3)
P 		168
B 581.1
(3)
P 		330.4
(-2.8)
P 		321.4
(-3)
P 		432.4
(-0.4)
P 		260
C 550.3
(3)
P 		328.3
(-3)
P 		419.5
(-0.5)
P 		442.3
(0.1)
P 		222
avr 540.3
(3) 		353.4
(-3) 		359.9
(-2.8) 		460.6
(0.4) 		187

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 355
1 2 3 4 5 6 range
A 731.4
(3)
P 		500.3
(-0.9)
P 		379.3
(-3)
P 		478.3
(-1.3)
P 		570.6
(0.3)
P 		537.3
(-0.3)
P 		352
B 656.2
(3)
P 		618.3
(2.2)
P 		376.2
(-3)
P 		499.2
(-0.4)
P 		467.6
(-1)
P 		564.8
(1)
P 		280
C 605.6
(3)
P 		595.7
(2.7)
P 		415.2
(-3)
P 		462.3
(-1.5)
P 		443.1
(-2.1)
P 		502.6
(-0.2)
P 		190
avr 664.4
(3) 		571.4
(1) 		390.2
(-3) 		479.9
(-1) 		493.8
(-0.7) 		534.9
(0.2) 		274
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 111
1 2 3 4 range
A 178.3
(3)
P 		87.1
(-2.3)
P 		88.6
(-2.2)
P 		74.2
(-3)
P 		104
B 185.3
(3)
P 		81.6
(-2.6)
P 		74.5
(-3)
P 		92.2
(-2)
P 		111
C 182.2
(3)
P 		94.4
(-1.9)
P 		146.2
(1)
P 		74.6
(-3)
P 		108
avr 181.9
(3) 		87.7
(-2.6) 		103.1
(-1.7) 		80.3
(-3) 		102

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 146
1 2 3 4 5 6 range
A 134.8
(0.3)
P 		177.9
(3)
P 		82.6
(-3)
P 		121.3
(-0.6)
P 		172.2
(2.6)
P 		130.8
(0)
P 		95
B 228.3
(3)
P 		205.8
(2)
P 		91.4
(-3)
P 		132.2
(-1.2)
P 		190
(1.3)
P 		210.6
(2.2)
P 		137
C 224.5
(3)
P 		181.8
(1.1)
P 		105.7
(-2.4)
P 		93
(-3)
P 		143.4
(-0.7)
P 		168.2
(0.4)
P 		132
avr 195.9
(3) 		188.5
(2.6) 		93.2
(-3) 		115.5
(-1.7) 		168.5
(1.4) 		169.9
(1.5) 		103
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188816_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6418
Sequence Type Exemplar sequence
Representative Public ID C38D4.5 NCBI
Target Description C38D4.5 /REP_DB=WormBase Gene ID /WP=CE00918 /TR=SW:P46941 /GB=CAA86318.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=chimaerin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065588(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065588 NCBI Caenorhabditis elegans C38D4.5 (chimaerin) mRNA, complete cds. 11/11 None
SNAP00000018665 ENSEMBL cdna:SNAP chromosome:CEL140:III:4796876:4802538:1 11/11 None
GENEFINDER00000018673 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4796876:4802538:1 11/11 None
C38D4.5.2 ENSEMBL cdna:known chromosome:CEL140:III:4796801:4802847:1 gene:C38D4.5 11/11 None
C38D4.5.1 ENSEMBL cdna:known chromosome:CEL140:III:4796803:4802859:1 gene:C38D4.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4796875-4802538(+) 100.0 100.0
Public Domain and Genome References
Gene Title chimaerin
Gene Symbol tag-325
Ensembl C38D4.5
Entrez Gene 175636 Entrez gene
SwissProt P46941 EMBL-EBI
WormBase CE00918 Wormbase
Functional Annotations
Ortholog
ID Title Organism
HG-U133_PLUS_2:225618_AT Rho GTPase activating protein 27 hs
HG-U133B:225618_AT Rho GTPase activating protein 27 hs
HG-U133_PLUS_2:227057_AT Rho GTPase activating protein 27 hs
HG-U133B:227057_AT Rho GTPase activating protein 27 hs
HU35KSUBA:AA480941_AT Rho GTPase activating protein 27 hs
HU35KSUBA:RC_AA480993_AT Rho GTPase activating protein 27 hs
HU35KSUBC:RC_AA496633_AT Rho GTPase activating protein 27 hs
U133_X3P:HS.60659.0.A1_3P_AT Rho GTPase activating protein 27 hs
U133_X3P:HS.180958.0.A1_3P_X_AT Rho GTPase activating protein 27 hs
U133_X3P:HS.180958.0.A1_3P_AT Rho GTPase activating protein 27 hs
U133_X3P:225618_3P_AT Rho GTPase activating protein 27 hs
HG-U95B:53925_AT Rho GTPase activating protein 27 hs
HG-U95B:57870_AT Rho GTPase activating protein 27 hs
HG-U133_PLUS_2:229424_S_AT Rho GTPase activating protein 27 hs
HG-U133B:229424_S_AT Rho GTPase activating protein 27 hs
U133_X3P:HS.180958.1.A1_3P_AT Rho GTPase activating protein 27 hs
HG-U95C:59789_AT Rho GTPase activating protein 27 hs
HG-U95E:73386_AT Rho GTPase activating protein 27 hs
MOE430A:1416758_AT Rho GTPase activating protein 27 mm
MOUSE430A_2:1416758_AT Rho GTPase activating protein 27 mm
MOUSE430_2:1416758_AT Rho GTPase activating protein 27 mm
MG-U74BV2:108073_AT Rho GTPase activating protein 27 mm
RAE230B:1380700_AT Rho GTPase activating protein 15 rn
RAT230_2:1380700_AT Rho GTPase activating protein 15 rn
RAE230A:1377032_AT Rho GTPase activating protein 15 rn
RAT230_2:1377032_AT Rho GTPase activating protein 15 rn
RAE230A:1377385_AT Rho GTPase activating protein 15 rn
RAT230_2:1377385_AT Rho GTPase activating protein 15 rn
RAE230B:1385319_AT Rho GTPase activating protein 15 rn
RAT230_2:1385319_AT Rho GTPase activating protein 15 rn
RG-U34C:RC_AA818035_AT Rho GTPase activating protein 15 rn
RG-U34C:RC_AI103506_AT Rho GTPase activating protein 15 rn
Protein Similarities
Method ID Description E-Value
blast CAA86318 Hypothetical protein C38D4.5 [Caenorhabditis elegans] ref|NP_497989.1| C38D4.5 [Caenorhabditis elegans] sp|P46941|YLE5_CAEEL Hypothetical protein C38D4.5 in chromosome III 0.0
blast CAE71128 Hypothetical protein CBG17982 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 1.7E-10
Pfam IPR001849 EMBL-EBI Pleckstrin-like 3.1E-18
Pfam IPR000198 EMBL-EBI RhoGAP domain 1.2E-67
Sequence
Target Sequence 
>C. ELEGANS:188816_S_AT
tggaaacctgtccgcagtccaaaaaattcgatgtcaagctgatcaagacaattataaagc
tcttgtgtctgaagaagatattcatgttcttactggtgctcttaaactctttttccgaga
gctaaccgatccacttttcccaatttctcttcataaggaatacacatcagctatgcaaat
gcccaacgcgaccactcgtttcaaaaaattcgaagaacttctaagtcgacttccaaacga
gaatcgtgaaacattgaaaatgttacttcgtcatttgaatcgtgtagcctcgcattcatc
acaaaatcgaatgcaacaacataatcttgctattgtttttggtccaacactattccataa
tggtgatggagccgtgaatttggcaaccaaaaataagaaagctggaaagaaggcgaaacc
ttcgaaaaaggaagaaactcaagcaactcctgtccaatcgaattctcatctcgccttctc
aatgattatgcaatcccaaattgttcaatatcttctcgaatcggcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGAAACCTGTCCGCAGTCCAAAAA 556 547 1956 Antisense
ATTCATGTTCTTACTGGTGCTCTTA 63 13 2035 Antisense
GGTGCTCTTAAACTCTTTTTCCGAG 255 501 2050 Antisense
TTTTTCCGAGAGCTAACCGATCCAC 427 569 2065 Antisense
GAACTTCTAAGTCGACTTCCAAACG 566 349 2170 Antisense
TCGTCATTTGAATCGTGTAGCCTCG 665 601 2223 Antisense
TGTAGCCTCGCATTCATCACAAAAT 85 559 2238 Antisense
GTTTTTGGTCCAACACTATTCCATA 478 447 2290 Antisense
GAAGAAACTCAAGCAACTCCTGTCC 503 337 2386 Antisense
TCCAATCGAATTCTCATCTCGCCTT 445 587 2408 Antisense
GTTCAATATCTTCTCGAATCGGCCA 29 447 2458 Antisense
Sequence Source Affymetrix Proprietary Database