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[188804_at] Calmodulin

Description F12A10.5 /REP_DB=WormBase Gene ID /WP=CE01908 /TR=Q09980 /GB=AAA68298.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Calmodulin
Cluster 6grp_high1_10_10 > 30      6grp_total > 73      4grp_total > 30      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 41.9
(-1.8)
P 		49.7
(3)
P 		40
(-3)
A 		45.8
(0.6)
A 		10
B 53.5
(3)
P 		25.7
(-3)
A 		40.8
(0.3)
A 		31.1
(-1.8)
M 		28
C 59.9
(2)
P 		42.3
(-0.7)
P 		27
(-3)
A 		66.5
(3)
P 		40
avr 51.8
(3) 		39.2
(-1.8) 		35.9
(-3) 		47.8
(1.5) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 11.5
(-3)
A 		34.1
(0.1)
P 		31.7
(-0.2)
M 		35.8
(0.4)
P 		51.3
(2.5)
P 		54.8
(3)
M 		43
B 26.2
(-2.4)
A 		39.2
(-0.8)
P 		70.9
(3)
P 		29
(-2)
M 		27.6
(-2.2)
P 		20.8
(-3)
A 		50
C 61.1
(3)
P 		48.8
(0.9)
P 		26.2
(-3)
A 		51.7
(1.4)
P 		45.6
(0.3)
P 		40
(-0.6)
P 		35
avr 32.9
(-3) 		40.7
(1.7) 		42.9
(3) 		38.8
(0.5) 		41.5
(2.2) 		38.5
(0.4) 		10
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 15.2
(3)
P 		10.8
(-0.1)
P 		10.5
(-0.3)
A 		6.7
(-3)
A 		9
B 17.1
(3)
P 		6.3
(-3)
A 		9.4
(-1.3)
A 		6.6
(-2.8)
M 		11
C 19.8
(3)
P 		12.2
(-1.4)
P 		9.4
(-3)
A 		11.2
(-2)
P 		10
avr 17.4
(3) 		9.8
(-2) 		9.8
(-2) 		8.2
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 2.1
(-3)
A 		12.1
(1.5)
P 		6.9
(-0.9)
M 		9.1
(0.1)
P 		15.5
(3)
P 		13.3
(2)
M 		13
B 9.1
(-2.1)
A 		13
(0.3)
P 		17.2
(3)
P 		7.7
(-3)
M 		11.2
(-0.8)
P 		7.8
(-2.9)
A 		10
C 22.6
(3)
P 		14.9
(0.1)
P 		6.7
(-3)
A 		10.4
(-1.6)
P 		14.8
(0.1)
P 		13.4
(-0.5)
P 		16
avr 11.3
(-0.2) 		13.3
(2.4) 		10.3
(-1.5) 		9.1
(-3) 		13.8
(3) 		11.5
(0.1) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188804_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5717
Sequence Type Exemplar sequence
Representative Public ID F12A10.5 NCBI
Target Description F12A10.5 /REP_DB=WormBase Gene ID /WP=CE01908 /TR=Q09980 /GB=AAA68298.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Calmodulin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062642(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062642 NCBI Caenorhabditis elegans F12A10.5 (Calmodulin) mRNA, complete cds. 11/11 None
SNAP00000033518 ENSEMBL cdna:SNAP chromosome:CEL140:II:5480657:5482006:-1 11/11 None
GENEFINDER00000033531 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5480657:5482006:-1 11/11 None
F12A10.5 ENSEMBL cdna:known chromosome:CEL140:II:5480580:5482006:-1 gene:F12A10.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5480577-5482004(-) 100.0 100.0
Public Domain and Genome References
Gene Title Calmodulin
Ensembl F12A10.5
Entrez Gene 184373 Entrez gene
SwissProt Q09980 EMBL-EBI
WormBase CE01908 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:260960_AT calcium-binding protein, putative at
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA68298 Hypothetical protein F12A10.5 [Caenorhabditis elegans] ref|NP_495043.1| F12A10.5 [Caenorhabditis elegans] 8.0E-78
blast CAE59115 Hypothetical protein CBG02410 [Caenorhabditis briggsae] 4.0E-77
blast AAA68298 Hypothetical protein F12A10.5 [Caenorhabditis elegans] ref|NP_495043.1| F12A10.5 [Caenorhabditis elegans] 1.0E-72
blast CAE59115 Hypothetical protein CBG02410 [Caenorhabditis briggsae] 6.0E-72
blast AAD34249 calmodulin mutant SYNCAM49 [synthetic construct] 5.0E-32
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 3.5E-9
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 2.4E-7
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.5E-7
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.1E-7
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.0014
Sequence
Target Sequence 
>C. ELEGANS:188804_AT
aaattcgtgaagtatttcgagagtttgataaaaatggagatggaagaattacaagacaag
agttggaggttgctcttcttcaattgggagaaaaagcgagcaattcgaagattgagacaa
tgattgagcaagctgatttagatggaaatggatgtattgatatagatgagtttttgaatg
tactccgacgacaaatctgtgatccgaaagaagaacgagaattgagagatgtttttaatg
tgttcgataaaaatggagacggcgtgatttccatcgatgatttaatttttgttatgtgcc
aattgggagagaagttaactgaaactgaagcgaaagaaatgataaaacaaggagatctag
atcatgacggaatgatcgattttcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATTCGTGAAGTATTTCGAGAGTT 627 113 35 Antisense
AGTTGGAGGTTGCTCTTCTTCAATT 297 63 95 Antisense
GGTTGCTCTTCTTCAATTGGGAGAA 590 507 102 Antisense
ATTGAGCAAGCTGATTTAGATGGAA 584 3 157 Antisense
AGTTTTTGAATGTACTCCGACGACA 481 61 203 Antisense
CTCCGACGACAAATCTGTGATCCGA 136 235 217 Antisense
GAGAGATGTTTTTAATGTGTTCGAT 621 395 258 Antisense
AGACGGCGTGATTTCCATCGATGAT 354 71 291 Antisense
GCGTGATTTCCATCGATGATTTAAT 49 291 296 Antisense
ATTTTTGTTATGTGCCAATTGGGAG 448 15 319 Antisense
TCATGACGGAATGATCGATTTTCAA 522 621 396 Antisense
Sequence Source Affymetrix Proprietary Database