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[188803_s_at]

Description C05D11.11 /REP_DB=WormBase Gene ID /WP=CE01130 /GEN=mel-32 /TR=SW:P50432 /GB=AAB53830.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 45      6grp_high1_10_10 > 53      4grp_highest_8_8 > 10      4grp_high_8_8 > 55      6grp_highest_nonorm > 56      6grp_total > 10      4grp_total > 48      6grp_cgc > 55      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3145
1 2 3 4 range
A 1805.6
(-3)
P 		4135.4
(2.7)
P 		4255.4
(3)
P 		1797.1
(-3)
P 		2458
B 1770.9
(-3)
P 		3697.6
(0.7)
P 		4915.6
(3)
P 		2101.2
(-2.4)
P 		3145
C 2044.3
(-2.8)
P 		3598.7
(0.5)
P 		4770.9
(3)
P 		1931.9
(-3)
P 		2839
avr 1873.6
(-3) 		3810.6
(1.2) 		4647.3
(3) 		1943.4
(-2.8) 		2774

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1337
1 2 3 4 5 6 range
A 1375.8
(-3)
P 		2706.6
(3)
P 		2110.8
(0.3)
P 		1774.9
(-1.2)
P 		1937.1
(-0.5)
P 		1782.9
(-1.2)
P 		1331
B 1583.7
(-3)
P 		2329.2
(1.4)
P 		2590.5
(3)
P 		1686.3
(-2.4)
P 		1894.6
(-1.1)
P 		2007.6
(-0.5)
P 		1007
C 1811.9
(-3)
P 		2072.4
(-1.3)
P 		2415.7
(1)
P 		1935.3
(-2.2)
P 		2096.5
(-1.1)
P 		2713.2
(3)
P 		901
avr 1590.5
(-3) 		2369.4
(3) 		2372.3
(3) 		1798.8
(-1.4) 		1976.1
(-0) 		2167.9
(1.4) 		782
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1400
1 2 3 4 range
A 657.4
(-0.2)
P 		897.7
(1.5)
P 		1112.4
(3)
P 		263.1
(-3)
P 		849
B 564.7
(-2)
P 		913.2
(1)
P 		1139.1
(3)
P 		447.8
(-3)
P 		691
C 676.7
(-1.4)
P 		1034.6
(0.2)
P 		1662.8
(3)
P 		325.8
(-3)
P 		1337
avr 632.9
(-1.2) 		948.5
(0.8) 		1304.8
(3) 		345.6
(-3) 		959

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 709
1 2 3 4 5 6 range
A 253.6
(-3)
P 		962.6
(3)
P 		459.7
(-1.3)
P 		450.2
(-1.3)
P 		584.5
(-0.2)
P 		434.1
(-1.5)
P 		709
B 551
(-1.1)
P 		775.3
(3)
P 		629
(0.3)
P 		446.6
(-3)
P 		769.8
(2.9)
P 		748.6
(2.5)
P 		329
C 671.6
(0.3)
P 		632.4
(-0.2)
P 		614.8
(-0.4)
P 		389.2
(-3)
P 		678.4
(0.3)
P 		908.3
(3)
P 		519
avr 492.1
(-1.9) 		790.1
(3) 		567.8
(-0.7) 		428.7
(-3) 		677.6
(1.1) 		697
(1.5) 		361
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188803_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7598
Sequence Type Exemplar sequence
Representative Public ID C05D11.11 NCBI
Target Description C05D11.11 /REP_DB=WormBase Gene ID /WP=CE01130 /GEN=mel-32 /TR=SW:P50432 /GB=AAB53830.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171169(11), NM_171170(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171169 NCBI Caenorhabditis elegans Maternal Effect Lethal family member (mel-32) (mel-32) mRNA, complete cds. 11/11 None
NM_171170 NCBI Caenorhabditis elegans Maternal Effect Lethal family member (mel-32) (mel-32) mRNA, complete cds. 11/11 None
SNAP00000019458 ENSEMBL cdna:SNAP chromosome:CEL140:III:6440199:6442752:-1 11/11 None
GENEFINDER00000019469 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6440199:6441798:-1 11/11 None
C05D11.11b.1 ENSEMBL cdna:known chromosome:CEL140:III:6440082:6442774:-1 gene:C05D11.11 11/11 None
C05D11.11b.2 ENSEMBL cdna:known chromosome:CEL140:III:6440082:6442759:-1 gene:C05D11.11 11/11 None
C05D11.11a.2 ENSEMBL cdna:known chromosome:CEL140:III:6440199:6441806:-1 gene:C05D11.11 11/11 None
C05D11.11a.1 ENSEMBL cdna:known chromosome:CEL140:III:6440199:6441804:-1 gene:C05D11.11 11/11 None
C05D11.11a.3 ENSEMBL cdna:known chromosome:CEL140:III:6440199:6441806:-1 gene:C05D11.11 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6440197-6441797(-) 100.0 100.0
Public Domain and Genome References
Ensembl C05D11.11
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL27228 Maternal effect lethal protein 32, isoform b [Caenorhabditis elegans] ref|NP_741197.1| Maternal Effect Lethal family member (mel-32) [Caenorhabditis elegans] sp|P50432|GLYC_CAEEL Serine hydroxymethyltransferase (Serine methylase) (Glycine hydroxymethyltransferase) (SHMT) (Glycosylation related 1) 0.0
blast AAB53830 Maternal effect lethal protein 32, isoform a [Caenorhabditis elegans] ref|NP_741198.1| Maternal Effect Lethal family member (mel-32) [Caenorhabditis elegans] 0.0
blast CAE72494 Hypothetical protein CBG19673 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001085 EMBL-EBI Glycine hydroxymethyltransferase 1.0E-126
Pfam IPR001085 EMBL-EBI Glycine hydroxymethyltransferase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:188803_S_AT
aggagaagatcaactcggccgtgttcccaggacttcaaggtggaccacacaatcacacta
ttgctggaattgccgttgctttgagacaatgcctttctgaagattttgttcagtacggag
agcaagtgttgaagaatgccaaaaccttggccgagagaatgaagaagcatggatatgctt
tggcaaccggaggaaccgacaatcatttgttgcttgttgacttgcgcccaatcggagttg
aaggagctcgtgctgagcatgtgcttgatctcgctcatattgcttgcaacaagaatacgt
gcccaggagacgtttctgctttgagaccaggaggcattcgtctcggaactccagctctca
cctcccgtggattccaggagca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGAGAAGATCAACTCGGCCGTGTT 580 55 884 Antisense
GGACCACACAATCACACTATTGCTG 133 523 925 Antisense
ATTGCTGGAATTGCCGTTGCTTTGA 272 7 943 Antisense
GTTGCTTTGAGACAATGCCTTTCTG 601 439 958 Antisense
ATGCTTTGGCAACCGGAGGAACCGA 484 29 1058 Antisense
CATTTGTTGCTTGTTGACTTGCGCC 227 215 1087 Antisense
GCTGAGCATGTGCTTGATCTCGCTC 260 295 1135 Antisense
GATCTCGCTCATATTGCTTGCAACA 157 419 1150 Antisense
TGCCCAGGAGACGTTTCTGCTTTGA 173 585 1183 Antisense
ACCAGGAGGCATTCGTCTCGGAACT 278 87 1209 Antisense
TCACCTCCCGTGGATTCCAGGAGCA 696 625 1241 Antisense
Sequence Source Affymetrix Proprietary Database