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[188802_at] DNAJ protein

Description F54D5.8 /REP_DB=WormBase Gene ID /WP=CE03412 /TR=Q20774 /GB=CAA91334.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=DNAJ protein
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 85      4grp_highest_8_8 > 21      4grp_high_8_8 > 23      6grp_highest_nonorm > 88      6grp_total > 86      4grp_total > 0      6grp_highest_sub > In      6grp_cgc > 58      6grp_cgc_sub > In      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1264
1 2 3 4 range
A 826.9
(-2.1)
P
659.3
(-3)
P
1341.2
(0.7)
P
1759.6
(3)
P
1100
B 731.9
(-2.9)
P
707.8
(-3)
P
1562.7
(1.2)
P
1922.9
(3)
P
1215
C 907.8
(-1.7)
P
684.5
(-3)
P
1479.2
(1.6)
P
1730.4
(3)
P
1046
avr 822.2
(-2.3)
683.9
(-3)
1461
(1.2)
1804.3
(3)
1120

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1217
1 2 3 4 5 6 range
A 879.6
(-3)
P
1376.4
(-0.1)
P
1129.9
(-1.5)
P
1203.7
(-1.1)
P
1534.3
(0.8)
P
1911.3
(3)
P
1032
B 694.1
(-3)
P
1343.9
(0.8)
P
1298.3
(0.5)
P
1409.8
(1.1)
P
1494.8
(1.6)
P
1729.5
(3)
P
1035
C 728.6
(-3)
P
1347.5
(0.3)
P
1255.4
(-0.2)
P
1261.6
(-0.2)
P
1261.9
(-0.2)
P
1864.5
(3)
P
1136
avr 767.4
(-3)
1355.9
(0.3)
1227.9
(-0.4)
1291.7
(-0.1)
1430.3
(0.7)
1835.1
(3)
1068
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 373
1 2 3 4 range
A 301.1
(1.6)
P
143.1
(-3)
P
350.6
(3)
P
257.6
(0.3)
P
208
B 233.4
(-1.5)
P
174.8
(-3)
P
362.1
(1.8)
P
409.8
(3)
P
235
C 300.5
(-1)
P
196.8
(-3)
P
515.6
(3)
P
291.8
(-1.2)
P
319
avr 278.3
(-0.3)
171.6
(-3)
409.4
(3)
319.7
(0.7)
238

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 483
1 2 3 4 5 6 range
A 162.1
(-3)
P
489.5
(3)
P
246.1
(-1.5)
P
305.3
(-0.4)
P
463
(2.5)
P
465.3
(2.6)
P
327
B 241.5
(-3)
P
447.3
(0.1)
P
315.3
(-1.9)
P
373.4
(-1)
P
607.3
(2.4)
P
644.9
(3)
P
403
C 270
(-2.7)
P
411.1
(-0.5)
P
319.5
(-1.9)
P
253.7
(-3)
P
408.3
(-0.5)
P
624.2
(3)
P
371
avr 224.5
(-3)
449.3
(0.8)
293.6
(-1.8)
310.8
(-1.5)
492.9
(1.6)
578.1
(3)
354
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188802_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3319
Sequence Type Exemplar sequence
Representative Public ID F54D5.8 NCBI
Target Description F54D5.8 /REP_DB=WormBase Gene ID /WP=CE03412 /TR=Q20774 /GB=CAA91334.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=DNAJ protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064067(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_064067 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-13) (dnj-13) mRNA, complete cds. 11/11 None
SNAP00000004666 ENSEMBL cdna:SNAP chromosome:CEL140:II:11545619:11546876:1 11/11 None
GENEFINDER00000004682 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11545619:11546876:1 11/11 None
F54D5.8.2 ENSEMBL cdna:known chromosome:CEL140:II:11545604:11547262:1 gene:F54D5.8 11/11 None
F54D5.8.1 ENSEMBL cdna:known chromosome:CEL140:II:11545606:11547262:1 gene:F54D5.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11545615-11546873(+) 100.0 100.0
Public Domain and Genome References
Gene Title DNAJ protein
Gene Symbol dnj-13
Ensembl F54D5.8
Entrez Gene 3564910 Entrez gene
SwissProt Q20774 EMBL-EBI
WormBase CE03412 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:246894_AT DNAJ heat shock protein, putative at
CANINE:1604150_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 4 cfa
CANINE_2:CFA.16255.1.S1_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 4 cfa
CANINE_2:CFAAFFX.31168.1.S1_S_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 4 cfa
DROSOPHILA_2:1637059_S_AT DnaJ-like-1 dm
DROSGENOME1:143835_AT DnaJ-like-1 dm
CHICKEN:GGA.12894.1.S1_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 4; DnaJ-like heat shock protein 40 gga
CHICKEN:GGA.13022.1.A1_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 4; DnaJ-like heat shock protein 40 gga
CHICKEN:GGAAFFX.22722.1.S1_AT similar to DnaJ (Hsp40) homolog, subfamily B, member 4; DnaJ-like heat shock protein 40 gga
HG-FOCUS:203810_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U133A_2:203810_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U133_PLUS_2:203810_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U133A:203810_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U95AV2:158_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HC-G110:158_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U133_PLUS_2:203811_S_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U133A_2:203811_S_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U133A:203811_S_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HU35KSUBA:RC_AA450303_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HUGENEFL:U40992_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
U133_X3P:G6631084_3P_A_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
U133_X3P:HS.41693.0.S2_3P_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U95AV2:33533_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U95C:62561_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
HG-U95E:86876_R_AT DnaJ (Hsp40) homolog, subfamily B, member 4 hs
MOUSE430A_2:1431734_A_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MOE430A:1431734_A_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MOUSE430_2:1431734_A_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MU11KSUBB:MSA.27940.0_S_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MU11KSUBB:MSA.27198.0_F_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MG-U74AV2:93853_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MG-U74BV2:114288_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MOE430A:1451177_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MOUSE430A_2:1451177_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MOUSE430_2:1451177_AT DnaJ (Hsp40) homolog, subfamily B, member 4 mm
MOE430B:1439524_AT DnaJ (Hsp40) homolog, subfamily B, member 4 (Dnajb4), mRNA mm
MOUSE430_2:1439524_AT DnaJ (Hsp40) homolog, subfamily B, member 4 (Dnajb4), mRNA mm
MU19KSUBB:TC28401_AT DnaJ (Hsp40) homolog, subfamily B, member 4 (Dnajb4), mRNA mm
MU19KSUBC:TC35461_AT DnaJ (Hsp40) homolog, subfamily B, member 4 (Dnajb4), mRNA mm
MU19KSUBC:TC38135_AT DnaJ (Hsp40) homolog, subfamily B, member 4 (Dnajb4), mRNA mm
RG-U34B:RC_AI008643_AT DnaJ (Hsp40) homolog, subfamily B, member 4 (predicted) rn
YEAST_2:1776450_AT Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; shares similarity with bacterial DnaJ proteins Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6457 protein folding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91334 Hypothetical protein F54D5.8 [Caenorhabditis elegans] ref|NP_496468.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-13) [Caenorhabditis elegans] 1.0E-139
blast CAA91334 Hypothetical protein F54D5.8 [Caenorhabditis elegans] ref|NP_496468.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-13) [Caenorhabditis elegans] 1.0E-136
blast CAE59478 Hypothetical protein CBG02862 [Caenorhabditis briggsae] 1.0E-126
blast CAE59478 Hypothetical protein CBG02862 [Caenorhabditis briggsae] 1.0E-123
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 2.00000000634215E-28
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 6.00000011306601E-31
Pfam IPR002939 EMBL-EBI Chaperone DnaJ, C-terminal 2.0E-19
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 2.4E-39
Pfam IPR002939 EMBL-EBI Chaperone DnaJ, C-terminal 2.0E-19
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 7.4E-24
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.0E-9
Sequence
Target Sequence
>C. ELEGANS:188802_AT
tatgttcggcggcatgcatcaaggtggaagacgtggacatgctcgtcaagatcctgctgt
gctccatgatctttctgtcagtttggaggatgtactgaaaggtacaacgaagaagatgaa
gattacaagaaaagttatgacggacaatgctcaacgcctagaagacaaagttttgacggt
cactattaaaccaggatggaagtcaggcacaaagatcacattcccaaaagaaggtgatca
gcacccgaacagaactcccgccgacatagtcttcgtcatcaaggataagccacacccgaa
attcaagcgagagggatctgacatcaaacgcgtcgaaaagatctccctgaagtctgcatt
gactggccttgatattatgattccaacgttggatggagcagactaccgtcttcaattgaa
cgatgtcattaagccgggaactacaagacggctgactggaaaaggacttccaaatccaaa
gtctccatctcacagaggagatttaatcattgagtttgacgtggaattcccatcacaact
ca


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
TATGTTCGGCGGCATGCATCAAGGT 208 655 429 Antisense
GTGGACATGCTCGTCAAGATCCTGC 139 491 461 Antisense
TGCTCCATGATCTTTCTGTCAGTTT 464 581 488 Antisense
TATGACGGACAATGCTCAACGCCTA 17 655 564 Antisense
GGTGATCAGCACCCGAACAGAACTC 271 501 661 Antisense
CGCCGACATAGTCTTCGTCATCAAG 4 257 687 Antisense
AAAGATCTCCCTGAAGTCTGCATTG 192 123 765 Antisense
TCTGCATTGACTGGCCTTGATATTA 551 609 781 Antisense
GATGGAGCAGACTACCGTCTTCAAT 518 407 820 Antisense
TTCCAAATCCAAAGTCTCCATCTCA 595 701 896 Antisense
GACGTGGAATTCCCATCACAACTCA 625 377 946 Antisense
Sequence Source Affymetrix Proprietary Database