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[188800_at] dehydrogenase

Description DC2.5 /REP_DB=WormBase Gene ID /WP=CE20634 /TR=Q9UAW2 /GB=AAD14726.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=dehydrogenase
Cluster 4grp_high_8_8 > 35      6grp_total > 13      4grp_total > 44      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 149
1 2 3 4 range
A 209.2
(3)
P 		208
(2.9)
A 		189.8
(1.8)
A 		112.6
(-3)
P 		97
B 252.6
(3)
P 		214.6
(1.5)
P 		156.2
(-0.9)
M 		103.5
(-3)
P 		149
C 192.2
(0.5)
P 		244.2
(3)
P 		126.1
(-2.8)
P 		121.4
(-3)
P 		123
avr 218
(2.8) 		222.3
(3) 		157.4
(-0.5) 		112.5
(-3) 		110

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 237
1 2 3 4 5 6 range
A 245.2
(3)
P 		240.4
(2.9)
P 		187.5
(1.2)
P 		226.1
(2.4)
P 		91.6
(-1.8)
A 		52.4
(-3)
A 		193
B 219.4
(3)
P 		167.3
(1)
P 		199.1
(2.2)
P 		203
(2.4)
P 		123.2
(-0.7)
A 		62.9
(-3)
A 		157
C 289.2
(3)
P 		192.5
(0)
M 		182.9
(-0.2)
P 		142.1
(-1.5)
A 		123.7
(-2.1)
A 		92.7
(-3)
A 		197
avr 251.3
(3) 		200.1
(1.3) 		189.8
(1) 		190.4
(1) 		112.8
(-1.6) 		69.3
(-3) 		182
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 76.2
(3)
P 		45.2
(-0.1)
A 		49.6
(0.3)
A 		16.5
(-3)
P 		60
B 80.6
(3)
P 		53
(0.2)
P 		36.2
(-1.6)
M 		22.1
(-3)
P 		59
C 63.6
(2.2)
P 		70.2
(3)
P 		44
(-0.2)
P 		20.5
(-3)
P 		50
avr 73.5
(3) 		56.1
(1.1) 		43.3
(-0.4) 		19.7
(-3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 94
1 2 3 4 5 6 range
A 45.2
(-0.3)
P 		85.5
(3)
P 		40.8
(-0.7)
P 		57.3
(0.7)
P 		27.7
(-1.8)
A 		12.8
(-3)
A 		73
B 76.3
(3)
P 		55.7
(0.7)
P 		48.3
(-0.2)
P 		53.8
(0.4)
P 		50.1
(0)
A 		23.4
(-3)
A 		53
C 107.2
(3)
P 		58.7
(-0.7)
M 		46.6
(-1.6)
P 		28.6
(-3)
A 		40
(-2.1)
A 		31
(-2.8)
A 		79
avr 76.2
(3) 		66.6
(1.9) 		45.2
(-0.5) 		46.6
(-0.3) 		39.3
(-1.1) 		22.4
(-3) 		54
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188800_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15392
Sequence Type Exemplar sequence
Representative Public ID DC2.5 NCBI
Target Description DC2.5 /REP_DB=WormBase Gene ID /WP=CE20634 /TR=Q9UAW2 /GB=AAD14726.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070754(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070754 NCBI Caenorhabditis elegans DC2.5 (dehydrogenase) mRNA, complete cds. 11/11 None
SNAP00000026550 ENSEMBL cdna:SNAP chromosome:CEL140:V:209045:211655:-1 11/11 None
GENEFINDER00000026560 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:209912:213714:-1 11/11 None
DC2.5 ENSEMBL cdna:known chromosome:CEL140:V:210066:211401:-1 gene:DC2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:210065-213491(-) 93.73 93.73
Public Domain and Genome References
Gene Title dehydrogenase
Ensembl DC2.5
Entrez Gene 183970 Entrez gene
SwissProt Q9UAW2 EMBL-EBI
WormBase CE36814 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503155 DC2.5 [Caenorhabditis elegans] gb|AAD14726.3| Hypothetical protein DC2.5 [Caenorhabditis elegans] 1.0E-152
blast NP_503155 DC2.5 [Caenorhabditis elegans] gb|AAD14726.3| Hypothetical protein DC2.5 [Caenorhabditis elegans] 1.0E-146
blast AAK68295 Hypothetical protein E04F6.15 [Caenorhabditis elegans] ref|NP_495501.1| E04F6.15 [Caenorhabditis elegans] 1.0E-144
blast AAK68295 Hypothetical protein E04F6.15 [Caenorhabditis elegans] ref|NP_495501.1| E04F6.15 [Caenorhabditis elegans] 1.0E-136
blast CAE67570 Hypothetical protein CBG13098 [Caenorhabditis briggsae] 1.0E-132
blast CAE67570 Hypothetical protein CBG13098 [Caenorhabditis briggsae] 1.0E-128
blast AAK68295 Hypothetical protein E04F6.15 [Caenorhabditis elegans] ref|NP_495501.1| E04F6.15 [Caenorhabditis elegans] 1.0E-119
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 7.6E-17
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 7.6E-17
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000026560 1 20-39
Sequence
Target Sequence 
>C. ELEGANS:188800_AT
tgaacgccggcgttcttggacgaaaagaaaaaacgactgctgatagatttgaagctcatt
ttggaataaatcatcttgctcattttttgctgataaaggagcttcttccagttcttcgat
cgtcagctccatcaagaattgtcatcctttcttcgacgcttagcaagttcacttcgatca
atcctgattccaaaattgaggagaagcttggcactctttgcccaaaaaacgcaactgagt
ggtattaccgattgtatgcaaaatcaaaaatgtgcaacatgctaattgcgtttaaattgc
atcgagatgaatttgaaaacggaataagtgtatattcggttcatccgggatccgcagttc
gcacgaatcttcatagagatgttccgttttggagtatttttaattttttgtcaattcctt
ttacaaagaatgcgagccagggagcagcaacaagtctttattgtgcagtacatccaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAACGCCGGCGTTCTTGGACGAAA 100 573 584 Antisense
GCTGATAAAGGAGCTTCTTCCAGTT 84 295 672 Antisense
TCGTCAGCTCCATCAAGAATTGTCA 262 605 703 Antisense
ATCCTTTCTTCGACGCTTAGCAAGT 271 39 727 Antisense
GCAAGTTCACTTCGATCAATCCTGA 316 321 746 Antisense
GAGGAGAAGCTTGGCACTCTTTGCC 612 403 781 Antisense
TATATTCGGTTCATCCGGGATCCGC 430 651 914 Antisense
ATCCGCAGTTCGCACGAATCTTCAT 446 37 933 Antisense
TTCATAGAGATGTTCCGTTTTGGAG 453 683 953 Antisense
GCGAGCCAGGGAGCAGCAACAAGTC 5 291 1015 Antisense
GTCTTTATTGTGCAGTACATCCAGA 275 469 1037 Antisense
Sequence Source Affymetrix Proprietary Database