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[188796_s_at]

Description C32A3.1 /REP_DB=WormBase Gene ID /WP=CE01505 /CHR=3 /FEA=Sanger Annotation /DEF=locus:sel-8 (HINXTON) SW:Q09260 protein_id:CAA88284.1
Cluster 6grp_total > 65      4grp_total > 38      6grp_cgc > 46      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 7.5
(3)
A 		3.9
(-1.3)
A 		2.5
(-3)
A 		3.6
(-1.7)
A 		5
B 6.7
(3)
A 		3.3
(-2.4)
A 		3.2
(-2.5)
A 		2.9
(-3)
A 		4
C 2.9
(0.3)
A 		3.1
(1.4)
A 		2.3
(-3)
A 		3.4
(3)
A 		1
avr 5.7
(3) 		3.4
(-1.6) 		2.7
(-3) 		3.3
(-1.8) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4
1 2 3 4 5 6 range
A 2
(-2.8)
A 		2.3
(-2.4)
A 		2.5
(-2.1)
A 		2.1
(-2.7)
A 		1.9
(-3)
A 		5.8
(3)
A 		4
B 2.3
(-0.4)
A 		2.2
(-0.9)
A 		2.4
(-0)
A 		3.1
(3)
A 		1.7
(-3)
A 		2.2
(-0.9)
A 		1
C 1.8
(-2.3)
A 		2.3
(-1.2)
A 		4.2
(3)
A 		2.7
(-0.3)
A 		1.5
(-3)
A 		1.7
(-2.6)
A 		3
avr 2
(-1.8) 		2.3
(-0.6) 		3
(2.2) 		2.6
(0.6) 		1.7
(-3) 		3.2
(3) 		2
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2
1 2 3 4 range
A 2.7
(3)
A 		0.8
(-2.2)
A 		0.7
(-2.5)
A 		0.5
(-3)
A 		2
B 2.1
(3)
A 		0.8
(-2.2)
A 		0.7
(-2.6)
A 		0.6
(-3)
A 		2
C 0.9
(3)
A 		0.9
(3)
A 		0.8
(1)
A 		0.6
(-3)
A 		0
avr 1.9
(3) 		0.8
(-2.1) 		0.7
(-2.5) 		0.6
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1
1 2 3 4 5 6 range
A 0.4
(-3)
A 		0.8
(-0.6)
A 		0.5
(-2.4)
A 		0.5
(-2.4)
A 		0.6
(-1.8)
A 		1.4
(3)
A 		1
B 0.8
(3)
A 		0.7
(-0)
A 		0.6
(-3)
A 		0.8
(3)
A 		0.7
(-0)
A 		0.8
(3)
A 		0
C 0.7
(-1)
A 		0.7
(-1)
A 		1.1
(3)
A 		0.5
(-3)
A 		0.5
(-3)
A 		0.6
(-2)
A 		1
avr 0.6
(-3) 		0.7
(-1) 		0.7
(-1) 		0.6
(-3) 		0.6
(-3) 		0.9
(3) 		0
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188796_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6574
Sequence Type Exemplar sequence
Representative Public ID C32A3.1 NCBI
Target Description C32A3.1 /REP_DB=WormBase Gene ID /WP=CE01505 /CHR=3 /FEA=Sanger Annotation /DEF=locus:sel-8 (HINXTON) SW:Q09260 protein_id:CAA88284.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026023(11), NM_001026024(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026023 NCBI Caenorhabditis elegans Suppressor/Enhancer of Lin-12 family member (sel-8) (sel-8) mRNA, complete cds. 11/11 None
NM_001026024 NCBI Caenorhabditis elegans Suppressor/Enhancer of Lin-12 family member (sel-8) (sel-8) mRNA, complete cds. 11/11 None
SNAP00000010768 ENSEMBL cdna:SNAP chromosome:CEL140:III:3613284:3619359:-1 11/11 None
GENEFINDER00000010777 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3613284:3630345:-1 11/11 None
C32A3.1a ENSEMBL cdna:known chromosome:CEL140:III:3613199:3627121:-1 gene:C32A3.1 11/11 None
C32A3.1b ENSEMBL cdna:known chromosome:CEL140:III:3613199:3618511:-1 gene:C32A3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3613283-3618501(-) 89.27 100.0
Public Domain and Genome References
Ensembl C32A3.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAC42265 Hypothetical protein C32A3.1a [Caenorhabditis elegans] ref|NP_001021194.1| Suppressor/Enhancer of Lin-12 family member (sel-8) [Caenorhabditis elegans] gb|AAF71522.1| LAG-3A [Caenorhabditis elegans] sp|Q09260|LAG3_CAEEL Lag-3 protein (Abnormal germ line proliferation protein 3) (Abnormal cell lineage protein 3) 1.0E-165
blast CAE64938 Hypothetical protein CBG09769 [Caenorhabditis briggsae] 1.0E-165
blast CAA88284 Hypothetical protein C32A3.1b [Caenorhabditis elegans] ref|NP_001021195.1| Suppressor/Enhancer of Lin-12 family member (sel-8) [Caenorhabditis elegans] gb|AAF71523.1| LAG-3B [Caenorhabditis elegans] 1.0E-154
blast CAC42265 Hypothetical protein C32A3.1a [Caenorhabditis elegans] ref|NP_001021194.1| Suppressor/Enhancer of Lin-12 family member (sel-8) [Caenorhabditis elegans] gb|AAF71522.1| LAG-3A [Caenorhabditis elegans] sp|Q09260|LAG3_CAEEL Lag-3 protein (Abnormal germ line proliferation protein 3) (Abnormal cell lineage protein 3) 1.0E-154
Sequence
Target Sequence 
>C. ELEGANS:188796_S_AT
tattgggtccggatacctacgacacatttctagccgaactggacttttcatcctccgaat
caccaacgaagcagtctccgatggaaatgaatggcgatcggatgccgtcgacggctccac
caccagctcagaatccacaacacattgctcagcttcaacagcagcaaaataagatgagat
tgatgcagcaacagcaacaggaaatgcaacgaattgaacagcaaaggaggcagcagatta
tgcagcaacaacaacagcagcagcaacaagaacatcaacgacaacagatgcttctacagc
agcagcaacaacaacaacaaatgcagcaacatcatcaaatgaatggaggtggacaatttg
cgacgcaggcacatcagcaggctgcttatatgcagcaaatgcagagaatggagcagattc
gacaccaacaacaacaagcccaacaacatcaacaggctcaacagcaacatcagcagcaag
ctcaacatcatcaaatgggttacggtattccgaatggatatccacagcaaatgcatatgc
atccaccggcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATTGGGTCCGGATACCTACGACAC 5 663 818 Antisense
CCTACGACACATTTCTAGCCGAACT 251 265 833 Antisense
TAGCCGAACTGGACTTTTCATCCTC 562 651 848 Antisense
ATCCTCCGAATCACCAACGAAGCAG 204 37 867 Antisense
CAACGAAGCAGTCTCCGATGGAAAT 666 187 881 Antisense
TGAATGGCGATCGGATGCCGTCGAC 702 575 905 Antisense
AATCCACAACACATTGCTCAGCTTC 601 167 949 Antisense
CAGATGCTTCTACAGCAGCAGCAAC 576 205 1102 Antisense
AATTTGCGACGCAGGCACATCAGCA 393 171 1172 Antisense
ATGGAGCAGATTCGACACCAACAAC 47 53 1225 Antisense
GCAAATGCATATGCATCCACCGGCC 250 317 1344 Antisense
Sequence Source Affymetrix Proprietary Database