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[188761_s_at]

Description Y110A7A.6 /REP_DB=WormBase Gene ID /WP=CE23252 /CHR=1 /FEA=Sanger Annotation /DEF=phosphofructokinase (ST.LOUIS) protein_id:AAF60424.1
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 62      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 45      6grp_total > 62      4grp_total > 20      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 887
1 2 3 4 range
A 1985.1
(1.9)
P 		1926.1
(1.3)
P 		1519.8
(-3)
P 		2090.8
(3)
P 		571
B 1856.4
(0.1)
P 		1886.1
(0.3)
P 		1438.6
(-3)
P 		2248.1
(3)
P 		810
C 1856.8
(-0.6)
P 		1541.1
(-3)
P 		1702.8
(-1.8)
P 		2325.3
(3)
P 		784
avr 1899.4
(0.1) 		1784.4
(-0.9) 		1553.7
(-3) 		2221.4
(3) 		668

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1548
1 2 3 4 5 6 range
A 1428.2
(-3)
P 		2523.1
(1.2)
P 		2975.8
(3)
P 		2882.5
(2.6)
P 		2072.4
(-0.5)
P 		1976
(-0.9)
P 		1548
B 1459.5
(-3)
P 		2240.7
(0.4)
P 		2852.1
(3)
P 		2457.1
(1.3)
P 		2183.9
(0.1)
P 		2301.5
(0.6)
P 		1393
C 1562.3
(-3)
P 		2450.5
(1.2)
P 		2808.4
(2.8)
P 		2843.3
(3)
P 		2163.5
(-0.2)
P 		2122.4
(-0.4)
P 		1281
avr 1483.3
(-3) 		2404.8
(1) 		2878.8
(3) 		2727.6
(2.3) 		2139.9
(-0.2) 		2133.3
(-0.2) 		1396
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 417
1 2 3 4 range
A 722.8
(3)
P 		418.1
(-1.4)
P 		397.3
(-1.7)
P 		306.1
(-3)
P 		417
B 592
(3)
P 		465.8
(0.1)
P 		333.4
(-3)
P 		479.1
(0.4)
P 		259
C 614.7
(3)
P 		443.1
(-1.6)
P 		593.5
(2.4)
P 		392.1
(-3)
P 		223
avr 643.2
(3) 		442.3
(-1.8) 		441.4
(-1.8) 		392.4
(-3) 		251

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 634
1 2 3 4 5 6 range
A 263.3
(-3)
P 		897.3
(3)
P 		648.1
(0.6)
P 		731.1
(1.4)
P 		625.3
(0.4)
P 		481.1
(-0.9)
P 		634
B 507.8
(-3)
P 		745.9
(0.8)
P 		692.6
(-0.1)
P 		650.8
(-0.7)
P 		887.3
(3)
P 		858.2
(2.5)
P 		380
C 579.1
(-2.8)
P 		747.7
(3)
P 		714.8
(1.9)
P 		571.8
(-3)
P 		700.1
(1.4)
P 		710.5
(1.7)
P 		176
avr 450.1
(-3) 		797
(3) 		685.2
(1.1) 		651.2
(0.5) 		737.6
(2) 		683.3
(1) 		347
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188761_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2180
Sequence Type Exemplar sequence
Representative Public ID Y110A7A.6 NCBI
Target Description Y110A7A.6 /REP_DB=WormBase Gene ID /WP=CE23252 /CHR=1 /FEA=Sanger Annotation /DEF=phosphofructokinase (ST.LOUIS) protein_id:AAF60424.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059129(11), NM_059128(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059129 NCBI Caenorhabditis elegans Y110A7A.6b (phosphofructokinase) mRNA, complete cds. 11/11 A 
NM_059128 NCBI Caenorhabditis elegans Y110A7A.6a (phosphofructokinase) mRNA, complete cds. 11/11 A 
SNAP00000029212 ENSEMBL cdna:SNAP chromosome:CEL140:I:5131525:5133923:1 11/11 None
GENEFINDER00000029221 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5131525:5133923:1 11/11 None
Y110A7A.6b.2 ENSEMBL cdna:known chromosome:CEL140:I:5131509:5134035:1 gene:Y110A7A.6 11/11 None
Y110A7A.6b.3 ENSEMBL cdna:known chromosome:CEL140:I:5131523:5134035:1 gene:Y110A7A.6 11/11 None
Y110A7A.6b.1 ENSEMBL cdna:known chromosome:CEL140:I:5131524:5134035:1 gene:Y110A7A.6 11/11 None
Y110A7A.6a ENSEMBL cdna:known chromosome:CEL140:I:5131534:5134102:1 gene:Y110A7A.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5131524-5133923(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y110A7A.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68884 Hypothetical protein Y110A7A.6b [Caenorhabditis elegans] ref|NP_491530.1| Y110A7A.6b [Caenorhabditis elegans] 0.0
blast AAF60424 Hypothetical protein Y110A7A.6a [Caenorhabditis elegans] ref|NP_491529.2| Y110A7A.6a [Caenorhabditis elegans] 0.0
blast CAE65745 Hypothetical protein CBG10830 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec 6P21_YEAST (P40433) 6-phosphofructo-2-kinase 1 (EC 2.7.1.105) (Phosphofructokinase 2 I) (6PF-2-K 1) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003094 EMBL-EBI Fructose-2,6-bisphosphatase 4.3E-120
Pfam IPR001345 EMBL-EBI Phosphoglycerate/bisphosphoglycerate mutase 4.8E-81
Pfam IPR003094 EMBL-EBI Fructose-2,6-bisphosphatase 4.3E-120
Pfam IPR001345 EMBL-EBI Phosphoglycerate/bisphosphoglycerate mutase 4.1E-82
Pfam IPR001345 EMBL-EBI Phosphoglycerate/bisphosphoglycerate mutase 4.1E-82
Sequence
Target Sequence 
>C. ELEGANS:188761_S_AT
tatcatggagcttgagagacaatccaatgtccttgtcatttctcatcaagcagttctccg
ttgcattcttgcctattttactaacaaaaatcgagatgatcttccatatctgaaggttcc
attgcatacagtcatcaagctcacaccaaaagcctattcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATCATGGAGCTTGAGAGACAATCC 240 641 1158 Antisense
GAGACAATCCAATGTCCTTGTCATT 701 391 1173 Antisense
ATGTCCTTGTCATTTCTCATCAAGC 280 49 1184 Antisense
GTCATTTCTCATCAAGCAGTTCTCC 590 467 1192 Antisense
TCAAGCAGTTCTCCGTTGCATTCTT 445 631 1203 Antisense
TTGCATTCTTGCCTATTTTACTAAC 127 705 1218 Antisense
AATCGAGATGATCTTCCATATCTGA 135 169 1246 Antisense
GATCTTCCATATCTGAAGGTTCCAT 222 421 1255 Antisense
GAAGGTTCCATTGCATACAGTCATC 275 335 1269 Antisense
TGCATACAGTCATCAAGCTCACACC 619 577 1280 Antisense
AAGCTCACACCAAAAGCCTATTCCT 316 151 1294 Antisense
Sequence Source Affymetrix Proprietary Database