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[188760_at]

Description Y110A7A.6 /REP_DB=WormBase Gene ID /WP=CE23252 /CHR=1 /FEA=Sanger Annotation /DEF=phosphofructokinase (ST.LOUIS) protein_id:AAF60424.1
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 72      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 45      6grp_total > 72      4grp_total > 12      6grp_highest_sub > 03_04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 962
1 2 3 4 range
A 1875.1
(1.1)
P 		1356.5
(-3)
P 		1420.1
(-2.5)
P 		2110
(3)
P 		754
B 1431.9
(-1.7)
P 		1562.1
(-0.9)
P 		1223.4
(-3)
P 		2185.2
(3)
P 		962
C 1645.1
(-0.9)
P 		1514
(-2.4)
P 		1453.9
(-3)
P 		2010.5
(3)
P 		557
avr 1650.7
(-0.7) 		1477.5
(-2.1) 		1365.8
(-3) 		2101.9
(3) 		736

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1527
1 2 3 4 5 6 range
A 1517.1
(-3)
P 		1863.4
(-1.6)
P 		2761.2
(2.2)
P 		2950.7
(3)
P 		1911.6
(-1.3)
P 		1837.9
(-1.7)
P 		1434
B 1423.7
(-3)
P 		1992.7
(0.9)
P 		2051
(1.3)
P 		2297
(3)
P 		2294
(3)
P 		1826.8
(-0.2)
P 		873
C 1595.2
(-3)
P 		1899.2
(-1.4)
P 		2727.1
(3)
P 		2681.2
(2.8)
P 		2069.9
(-0.5)
P 		1787.9
(-2)
P 		1132
avr 1512
(-3) 		1918.4
(-0.8) 		2513.1
(2.3) 		2643
(3) 		2091.8
(0.1) 		1817.5
(-1.4) 		1131
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 399
1 2 3 4 range
A 682.7
(3)
P 		294.5
(-3)
P 		371.2
(-1.8)
P 		308.9
(-2.8)
P 		388
B 456.6
(2.7)
P 		385.8
(0.4)
P 		283.5
(-3)
P 		465.7
(3)
P 		182
C 544.6
(3)
P 		435.3
(-0.2)
P 		506.7
(1.9)
P 		339
(-3)
P 		206
avr 561.3
(3) 		371.9
(-3) 		387.1
(-2.5) 		371.2
(-3) 		190

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 652
1 2 3 4 5 6 range
A 279.7
(-3)
P 		662.7
(1.9)
P 		601.3
(1.1)
P 		748.4
(3)
P 		576.9
(0.8)
P 		447.5
(-0.9)
P 		469
B 495.4
(-3)
P 		663.3
(-0.7)
P 		498
(-3)
P 		608.4
(-1.4)
P 		932
(3)
P 		681.2
(-0.4)
P 		437
C 591.3
(-1)
P 		579.5
(-1.4)
P 		694.1
(3)
P 		539.2
(-3)
P 		669.8
(2.1)
P 		598.5
(-0.7)
P 		155
avr 455.5
(-3) 		635.2
(1) 		597.8
(0.2) 		632
(0.9) 		726.2
(3) 		575.7
(-0.3) 		271
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188760_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2180
Sequence Type Exemplar sequence
Representative Public ID Y110A7A.6 NCBI
Target Description Y110A7A.6 /REP_DB=WormBase Gene ID /WP=CE23252 /CHR=1 /FEA=Sanger Annotation /DEF=phosphofructokinase (ST.LOUIS) protein_id:AAF60424.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059129(10), NM_059128(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059129 NCBI Caenorhabditis elegans Y110A7A.6b (phosphofructokinase) mRNA, complete cds. 10/11 A 
NM_059128 NCBI Caenorhabditis elegans Y110A7A.6a (phosphofructokinase) mRNA, complete cds. 11/11 A 
SNAP00000029212 ENSEMBL cdna:SNAP chromosome:CEL140:I:5131525:5133923:1 11/11 None
GENEFINDER00000029221 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5131525:5133923:1 11/11 None
Y110A7A.6b.2 ENSEMBL cdna:known chromosome:CEL140:I:5131509:5134035:1 gene:Y110A7A.6 10/11 None
Y110A7A.6b.3 ENSEMBL cdna:known chromosome:CEL140:I:5131523:5134035:1 gene:Y110A7A.6 10/11 None
Y110A7A.6b.1 ENSEMBL cdna:known chromosome:CEL140:I:5131524:5134035:1 gene:Y110A7A.6 10/11 None
Y110A7A.6a ENSEMBL cdna:known chromosome:CEL140:I:5131534:5134102:1 gene:Y110A7A.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5131524-5133923(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y110A7A.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68884 Hypothetical protein Y110A7A.6b [Caenorhabditis elegans] ref|NP_491530.1| Y110A7A.6b [Caenorhabditis elegans] 0.0
blast AAF60424 Hypothetical protein Y110A7A.6a [Caenorhabditis elegans] ref|NP_491529.2| Y110A7A.6a [Caenorhabditis elegans] 0.0
blast CAE65745 Hypothetical protein CBG10830 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec 6P21_YEAST (P40433) 6-phosphofructo-2-kinase 1 (EC 2.7.1.105) (Phosphofructokinase 2 I) (6PF-2-K 1) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003094 EMBL-EBI Fructose-2,6-bisphosphatase 4.3E-120
Pfam IPR001345 EMBL-EBI Phosphoglycerate/bisphosphoglycerate mutase 4.8E-81
Pfam IPR003094 EMBL-EBI Fructose-2,6-bisphosphatase 4.3E-120
Pfam IPR001345 EMBL-EBI Phosphoglycerate/bisphosphoglycerate mutase 4.1E-82
Pfam IPR001345 EMBL-EBI Phosphoglycerate/bisphosphoglycerate mutase 4.1E-82
Sequence
Target Sequence 
>C. ELEGANS:188760_AT
attggtcgtcttggtggtgattccccactcagtgaaaatggtttaaattacgctggaaaa
cttcgcgaatacttcgaaaaagaagatttggacgatttccgtgtttggtcatctcaaaag
attcgtgccgcccagacagcttctcatctgaaagatctcgctggtcatacagagttctgg
aaatgtcttgatgaaatcgatgccggaatatgtgaaggattaacttatgaagatttcgag
gctcgttatccgaaacaattcgctgaaagagataaggataaatatcactaccgttatcca
agtggggaatcatatgaagatctggttgctcgtctggagccagttatcatggagcttgag
agacaatccaatgtccttgtcatttctcatcaagcagttctccgttgcattcttgcctat
tttactaacaaaaatcgagatgatcttccatatctgaaggttccattgcatacagtcatc
aagctcacaccaaaagcctattcctgtgagattgaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTGGTCGTCTTGGTGGTGATTCCC 339 3 814 Antisense
TTGGTGGTGATTCCCCACTCAGTGA 203 709 824 Antisense
GATTTGGACGATTTCCGTGTTTGGT 521 425 898 Antisense
TCATCTCAAAAGATTCGTGCCGCCC 438 619 922 Antisense
GCCCAGACAGCTTCTCATCTGAAAG 135 287 943 Antisense
GATCTCGCTGGTCATACAGAGTTCT 465 419 967 Antisense
GAGGCTCGTTATCCGAAACAATTCG 589 405 1051 Antisense
TAAATATCACTACCGTTATCCAAGT 599 639 1092 Antisense
TGAAGATCTGGTTGCTCGTCTGGAG 79 575 1128 Antisense
GTCTGGAGCCAGTTATCATGGAGCT 87 473 1145 Antisense
AAAGCCTATTCCTGTGAGATTGAAC 34 125 1306 Antisense
Sequence Source Affymetrix Proprietary Database