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[188740_at] histone H1

Description B0414.3 /REP_DB=WormBase Gene ID /WP=CE07733 /TR=O01833 /GB=AAB57720.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=histone H1
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 98      4grp_high_8_8 > 55      6grp_highest_nonorm > 69      6grp_total > 98      4grp_total > 48      6grp_highest_sub > In      6grp_cgc > 78      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1893
1 2 3 4 range
A 308.5
(-3)
M 		1304.6
(0.6)
P 		1969.6
(3)
P 		591.7
(-2)
P 		1661
B 312.5
(-3)
P 		1155.7
(-0.3)
P 		2201.3
(3)
P 		643.8
(-1.9)
P 		1889
C 371.1
(-3)
M 		1162.3
(0.4)
P 		1754.3
(3)
P 		490.5
(-2.5)
P 		1383
avr 330.7
(-3) 		1207.5
(0.2) 		1975.1
(3) 		575.3
(-2.1) 		1644

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4388
1 2 3 4 5 6 range
A 322.2
(-3)
P 		527.3
(-2.7)
P 		693.9
(-2.5)
P 		1472.1
(-1.4)
P 		3527.5
(1.4)
P 		4710.3
(3)
P 		4388
B 398.3
(-3)
P 		555.1
(-2.7)
P 		947.9
(-2.1)
P 		1368.3
(-1.4)
P 		3522.2
(2.1)
P 		4064.5
(3)
P 		3666
C 502.7
(-2.9)
P 		466.1
(-3)
P 		868.3
(-2.3)
P 		1312.1
(-1.6)
P 		4163.5
(3)
P 		3278.6
(1.6)
P 		3697
avr 407.7
(-3) 		516.2
(-2.8) 		836.7
(-2.3) 		1384.2
(-1.4) 		3737.7
(2.5) 		4017.8
(3) 		3610
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 529
1 2 3 4 range
A 112.3
(-2.6)
M 		283.2
(-0.2)
P 		514.9
(3)
P 		86.6
(-3)
P 		428
B 99.7
(-3)
P 		285.4
(-0.3)
P 		510.1
(3)
P 		137.2
(-2.5)
P 		410
C 122.9
(-2.5)
M 		334.2
(-0.1)
P 		611.4
(3)
P 		82.7
(-3)
P 		529
avr 111.6
(-2.9) 		300.9
(-0.3) 		545.5
(3) 		102.2
(-3) 		443

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1456
1 2 3 4 5 6 range
A 59.4
(-3)
P 		187.5
(-2.3)
P 		151.1
(-2.5)
P 		373.4
(-1.3)
P 		1064.4
(2.5)
P 		1146.8
(3)
P 		1087
B 138.6
(-3)
P 		184.8
(-2.8)
P 		230.2
(-2.6)
P 		362.4
(-2)
P 		1431
(2.6)
P 		1515.6
(3)
P 		1377
C 186.3
(-2.8)
P 		142.2
(-3)
P 		221
(-2.6)
P 		263.9
(-2.4)
P 		1347.2
(3)
P 		1097.5
(1.8)
P 		1205
avr 128.1
(-3) 		171.5
(-2.8) 		200.8
(-2.6) 		333.2
(-1.9) 		1280.9
(3) 		1253.3
(2.9) 		1153
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188740_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1021
Sequence Type Exemplar sequence
Representative Public ID B0414.3 NCBI
Target Description B0414.3 /REP_DB=WormBase Gene ID /WP=CE07733 /TR=O01833 /GB=AAB57720.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=histone H1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059277(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059277 NCBI Caenorhabditis elegans HIstone H1 Like family member (hil-5) (hil-5) mRNA, complete cds. 11/11 None
SNAP00000011710 ENSEMBL cdna:SNAP chromosome:CEL140:I:5782757:5783593:1 11/11 None
GENEFINDER00000011728 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5782757:5783593:1 11/11 None
B0414.3 ENSEMBL cdna:known chromosome:CEL140:I:5782724:5783746:1 gene:B0414.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5782756-5783593(+) 100.0 100.0
Public Domain and Genome References
Gene Title histone H1
Gene Symbol hil-5
Ensembl B0414.3
Entrez Gene 172242 Entrez gene
SwissProt O01833 EMBL-EBI
WormBase CE07733 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.17049.1.S1_AT similar to Histone H1.2 (H1d) cfa
HG-U133_PLUS_2:209398_AT histone 1, H1c hs
HG-U133A:209398_AT histone 1, H1c hs
HG-FOCUS:209398_AT histone 1, H1c hs
HG-U133A_2:209398_AT histone 1, H1c hs
HU35KSUBA:X57129_AT histone 1, H1c hs
HUGENEFL:X57129_AT histone 1, H1c hs
HU35KSUBD:RC_T90190_S_AT histone 1, H1c hs
U133_X3P:G12803628_3P_AT histone 1, H1c hs
HG-U95AV2:37018_AT histone 1, H1c hs
MU11KSUBB:MSA.751.0_AT histone 1, H1c mm
MG-U74AV2:94288_AT histone 1, H1c mm
MOE430A:1436994_A_AT histone 1, H1c mm
MOUSE430A_2:1436994_A_AT histone 1, H1c mm
MOUSE430_2:1436994_A_AT histone 1, H1c mm
MOE430A:1416101_A_AT histone 1, H1c mm
MOUSE430A_2:1416101_A_AT histone 1, H1c mm
MOUSE430_2:1416101_A_AT histone 1, H1c mm
MOUSE430A_2:1438204_AT Histone 1, H1c (Hist1h1c), mRNA mm
MOE430A:1438204_AT Histone 1, H1c (Hist1h1c), mRNA mm
MOUSE430_2:1438204_AT Histone 1, H1c (Hist1h1c), mRNA mm
MU19KSUBA:TC23367_S_AT Histone 1, H1c (Hist1h1c), mRNA mm
MU19KSUBA:TC23617_F_AT Histone 1, H1c (Hist1h1c), mRNA mm
MU19KSUBC:TC37549_AT Histone 1, H1c (Hist1h1c), mRNA mm
MU11KSUBB:W29553_AT Histone 1, H1c (Hist1h1c), mRNA mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6334 nucleosome assembly inferred from electronic annotation QuickGO AmiGO
7001 chromosome organization and biogenesis (sensu Eukaryota) inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
786 nucleosome inferred from electronic annotation QuickGO AmiGO
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB57720 Histone h1 like protein 5 [Caenorhabditis elegans] ref|NP_491678.1| HIstone H1 Like family member (hil-5) [Caenorhabditis elegans] sp|O01833|H15_CAEEL Histone H1.5 (Histone H1-like protein 5) gb|AAB81030.1| histone H1.5 [Caenorhabditis elegans] 2.0E-45
blast AAB81029 histone H1.4 [Caenorhabditis elegans] gb|AAC17754.1| Histone h1 like protein 4 [Caenorhabditis elegans] ref|NP_504277.1| HIstone H1 Like family member (hil-4) [Caenorhabditis elegans] sp|O17536|H14_CAEEL Histone H1.4 (Histone H1-like protein 4) 4.0E-27
blast AAB82588 histone H1.4 [Caenorhabditis elegans] 4.0E-27
Protein Domains
Database ID Description E-Value
scop a.4.5.Histone H1/H5 All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Histone H1/H5 1.99999996490334E-14
Pfam IPR005818 EMBL-EBI Histone H1/H5 6.9E-31
Sequence
Target Sequence 
>C. ELEGANS:188740_AT
gaaggccaaggctaccaagattccgaaggtgaaggtcgttgccgcccatccgccattcat
caacatgatcaccgaagccgtcagcaacctcaaggaccgcaagggatcatccagggtcgc
cattttcaagttcatcactgccaaatacacgcttggagatcaagttaacaagaccaacgc
tcaccttcgctctgcacttaaaaaaggagtcgtcagcaaggtcctcgtccagactaacgg
aatcggagccaacggacgattccgtcttgccgttgccgagaagccaccagcagtgaagaa
ggctgccactggagagcaaaaggttatgaagaccgtcgccaagaaggctgtcagcggaga
caaggctaagaaaactgtcgccaaaaagaccggagacaaggtgaagaaggtcaagtcacc
gaagaggatcgctaagccagctgtcaagaaggtcactaagaaagccgccgctccaacgaa
gtctgctgccaatgaaaccgctccaaagaaggctgctgccactgaagccgctccaaagaa
ggctgctgtcacgaaagctgct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGGCCAAGGCTACCAAGATTCCG 61 331 78 Antisense
TCCGAAGGTGAAGGTCGTTGCCGCC 421 591 99 Antisense
CATCAACATGATCACCGAAGCCGTC 554 213 135 Antisense
TCAGCAACCTCAAGGACCGCAAGGG 567 623 158 Antisense
ACCGCAAGGGATCATCCAGGGTCGC 278 85 173 Antisense
TCACCTTCGCTCTGCACTTAAAAAA 169 597 258 Antisense
GGTCCTCGTCCAGACTAACGGAATC 23 1 297 Antisense
AGAGGATCGCTAAGCCAGCTGTCAA 1 69 500 Antisense
AGAAGGTCACTAAGAAAGCCGCCGC 56 75 524 Antisense
AAGTCTGCTGCCAATGAAACCGCTC 519 157 556 Antisense
GAAGGCTGCTGTCACGAAAGCTGCT 567 333 615 Antisense
Sequence Source Affymetrix Proprietary Database