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[188737_at]

Description F57B10.10 /REP_DB=WormBase Gene ID /WP=CE11316 /GEN=dad-1 /TR=SW:P52872 /GB=AAB96727.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 07      6grp_high1_10_10 > 70      4grp_highest_8_8 > 02      4grp_high_8_8 > 54      6grp_highest_nonorm > 36      6grp_total > 71      4grp_total > 57      6grp_highest_sub > 04      6grp_cgc > 84      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2948
1 2 3 4 range
A 3655.4
(-2.8)
P 		5816.6
(2)
P 		6248.6
(3)
P 		3551.7
(-3)
P 		2697
B 3663.2
(-3)
P 		5881.8
(2.3)
P 		6155
(3)
P 		3678.7
(-3)
P 		2492
C 3490.5
(-2.4)
P 		5321.8
(3)
P 		5225.2
(2.7)
P 		3301.1
(-3)
P 		2021
avr 3603
(-2.8) 		5673.4
(2.5) 		5876.3
(3) 		3510.5
(-3) 		2366

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2030
1 2 3 4 5 6 range
A 4745.5
(2)
P 		4475.5
(0.6)
P 		4932.2
(3)
P 		4611.5
(1.3)
P 		4470.2
(0.5)
P 		3808.1
(-3)
P 		1124
B 4004.6
(-1.7)
P 		4599.5
(1.2)
P 		3729.8
(-3)
P 		4983.7
(3)
P 		4703.3
(1.7)
P 		4013.8
(-1.6)
P 		1254
C 4129.9
(-0.9)
P 		4638.7
(0.6)
P 		4464.1
(0.1)
P 		5457
(3)
P 		3670.8
(-2.3)
P 		3427.1
(-3)
P 		2030
avr 4293.3
(-0.4) 		4571.2
(0.9) 		4375.4
(-0) 		5017.4
(3) 		4281.4
(-0.5) 		3749.7
(-3) 		1268
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1301
1 2 3 4 range
A 1330.9
(1.4)
P 		1262.6
(1)
P 		1633.5
(3)
P 		519.9
(-3)
P 		1114
B 1168.2
(0.4)
P 		1452.7
(3)
P 		1426.2
(2.8)
P 		783.9
(-3)
P 		669
C 1155.5
(-0.2)
P 		1530
(1.6)
P 		1821.1
(3)
P 		556.6
(-3)
P 		1265
avr 1218.2
(0.6) 		1415.1
(1.7) 		1626.9
(3) 		620.1
(-3) 		1007

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1036
1 2 3 4 5 6 range
A 874.8
(-3)
P 		1591.7
(3)
P 		1074.1
(-1.3)
P 		1169.6
(-0.5)
P 		1348.9
(1)
P 		927.2
(-2.6)
P 		717
B 1393.4
(-0.1)
P 		1531
(0.7)
P 		905.7
(-3)
P 		1320
(-0.5)
P 		1910.9
(3)
P 		1496.7
(0.5)
P 		1005
C 1530.8
(3)
P 		1415.4
(1.4)
P 		1136.2
(-2.5)
P 		1097.4
(-3)
P 		1187.8
(-1.7)
P 		1147.3
(-2.3)
P 		433
avr 1266.3
(-0.1) 		1512.7
(3) 		1038.7
(-3) 		1195.7
(-1) 		1482.5
(2.6) 		1190.4
(-1.1) 		474
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188737_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.581
Sequence Type Exemplar sequence
Representative Public ID F57B10.10 NCBI
Target Description F57B10.10 /REP_DB=WormBase Gene ID /WP=CE11316 /GEN=dad-1 /TR=SW:P52872 /GB=AAB96727.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059488(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059488 NCBI Caenorhabditis elegans DAD (Defender against Apoptotic Death) homolog family member (dad-1) (dad-1) mRNA, complete cds. 11/11 None
GENEFINDER00000011845 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6561748:6562423:-1 11/11 None
F57B10.10.1 ENSEMBL cdna:known chromosome:CEL140:I:6561613:6562424:-1 gene:F57B10.10 11/11 None
F57B10.10.2 ENSEMBL cdna:known chromosome:CEL140:I:6561626:6562426:-1 gene:F57B10.10 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000011829 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6561676-6562352(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dad-1
Ensembl F57B10.10
Entrez Gene 172370 Entrez gene
SwissProt P52872 EMBL-EBI
WormBase CE11316 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:245791_AT defender against cell death 1 (DAD1) at
ATGENOME1:19665_AT defender against cell death 1 (DAD1) at
CANINE:1583382_S_AT similar to defender against cell death 1 cfa
CANINE_2:CFA.6064.1.A1_AT similar to defender against cell death 1 cfa
CANINE_2:CFAAFFX.17372.1.S1_AT similar to defender against cell death 1 cfa
CANINE_2:CFAAFFX.17372.1.S1_S_AT similar to defender against cell death 1 cfa
DROSOPHILA_2:1631595_AT dm
DROSGENOME1:146029_AT dm
CHICKEN:GGA.4960.1.S1_AT defender against cell death 1 gga
PLASMODIUM_ANOPHELES:200046_AT defender against cell death 1 hs
HG-U133_PLUS_2:200046_AT defender against cell death 1 hs
HG-U133A:200046_AT defender against cell death 1 hs
HG-U133B:200046_AT defender against cell death 1 hs
HG-FOCUS:200046_AT defender against cell death 1 hs
HG-U133A_2:200046_AT defender against cell death 1 hs
U133_X3P:200046_AT defender against cell death 1 hs
HUGENEFL:D15057_AT defender against cell death 1 hs
U133_X3P:G4503252_3P_S_AT defender against cell death 1 hs
HG-U95AV2:38413_AT defender against cell death 1 hs
MOE430A:1418528_A_AT defender against cell death 1 mm
MOUSE430A_2:1418528_A_AT defender against cell death 1 mm
MOUSE430_2:1418528_A_AT defender against cell death 1 mm
MOUSE430_2:1454860_X_AT defender against cell death 1 mm
MOE430A:1454860_X_AT defender against cell death 1 mm
MOUSE430A_2:1454860_X_AT defender against cell death 1 mm
MU11KSUBA:U84211_S_AT defender against cell death 1 mm
MU11KSUBB:MSA.23697.0_S_AT defender against cell death 1 mm
MG-U74AV2:96008_AT defender against cell death 1 mm
MG-U74CV2:167324_AT defender against cell death 1 mm
MU11KSUBB:MSA.5804.0_AT Defender against cell death 1, mRNA (cDNA clone MGC:36083 IMAGE:5003022) mm
RT-U34:Y13336CDS_AT defender against cell death 1 rn
RG-U34A:Y13336CDS_AT defender against cell death 1 rn
RT-U34:Y13336CDS_G_AT defender against cell death 1 rn
RG-U34A:Y13336CDS_G_AT defender against cell death 1 rn
RG-U34A:RC_AI013627_AT defender against cell death 1 rn
RAE230A:1367465_AT defender against cell death 1 rn
RAE230B:1367465_AT defender against cell death 1 rn
RAT230_2:1367465_AT defender against cell death 1 rn
Protein Similarities
Method ID Description E-Value
blast AAB96727 Dad (defender against apoptotic death) homolog protein 1 [Caenorhabditis elegans] ref|NP_491889.1| DAD (Defender against Apoptotic Death) homolog family member (dad-1) [Caenorhabditis elegans] sp|P52872|DAD1_CAEEL Defender against cell death 1 (Protein dad-1) emb|CAA61451.1| dad-1 [Caenorhabditis elegans] 2.0E-57
blast CAE67222 Hypothetical protein CBG12659 [Caenorhabditis briggsae] 1.0E-53
blast XP_783500 PREDICTED: similar to defender against cell death 1 [Strongylocentrotus purpuratus] 1.0E-38
Protein Domains
Database ID Description E-Value
Pfam IPR003038 EMBL-EBI Defender against death DAD protein 3.3E-97
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491889.1 3 29-51,56-78,93-112
F57B10.10.1 3 29-51,56-78,93-112
F57B10.10.2 3 29-51,56-78,93-112
GENEFINDER00000011845 3 29-51,56-78,93-112
Sequence
Target Sequence 
>C. ELEGANS:188737_AT
atggcggctcaagtagttccagttctctcgaaactgttcgatgactaccaaaagacaaca
tcatcgaagctgaaaatcattgatgcttacatgacatacatcctgttcaccggaatcttt
caattcatttattgcttgctcgtcggtacattcccattcaactcgttcctctctggtttc
atctccactgtcacttcgttcgttttagcatcgtgtctcaggatgcaagtaaatcaagag
aaccgctccgagttcacagctgtctcgacagaacgtgcatttgctgatttcatcttcgca
aacctcatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGCGGCTCAAGTAGTTCCAGTTC 96 53 13 Antisense
GTTCCAGTTCTCTCGAAACTGTTCG 340 437 28 Antisense
TACATGACATACATCCTGTTCACCG 567 643 100 Antisense
ATCCTGTTCACCGGAATCTTTCAAT 400 39 112 Antisense
TTATTGCTTGCTCGTCGGTACATTC 248 679 141 Antisense
CACTGTCACTTCGTTCGTTTTAGCA 46 199 198 Antisense
GTTCGTTTTAGCATCGTGTCTCAGG 34 443 210 Antisense
CAAGAGAACCGCTCCGAGTTCACAG 23 185 247 Antisense
CCGAGTTCACAGCTGTCTCGACAGA 101 265 260 Antisense
GTGCATTTGCTGATTTCATCTTCGC 615 479 287 Antisense
TGATTTCATCTTCGCAAACCTCATT 100 565 297 Antisense
Sequence Source Affymetrix Proprietary Database