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[188736_s_at] peptidase

Description F18A12.6 /REP_DB=WormBase Gene ID /WP=CE09459 /TR=O16790 /GB=AAB66078.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=peptidase
Cluster 6grp_total > 90      4grp_total > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 2
(-2.8)
A 		5.9
(0.5)
A 		1.7
(-3)
A 		9
(3)
A 		7
B 1.6
(-3)
A 		1.9
(-2.4)
A 		3.4
(0.9)
A 		4.4
(3)
A 		3
C 8.8
(-1.8)
A 		3
(-3)
A 		5.5
(-2.5)
A 		31.9
(3)
A 		29
avr 4.1
(-2.7) 		3.6
(-2.9) 		3.5
(-3) 		15.1
(3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 32.1
(3)
A 		3.2
(-2.7)
A 		4.7
(-2.4)
A 		5.1
(-2.3)
A 		1.8
(-3)
A 		4.8
(-2.4)
A 		30
B 2.5
(-2.6)
A 		4.4
(-0.9)
A 		2.1
(-3)
A 		4.1
(-1.2)
A 		6
(0.6)
A 		8.6
(3)
A 		7
C 1.6
(-3)
A 		2.9
(-2.8)
A 		2.8
(-2.8)
A 		47.1
(3)
A 		2.3
(-2.9)
A 		7.7
(-2.2)
A 		46
avr 12.1
(0.4) 		3.5
(-2.9) 		3.2
(-3) 		18.8
(3) 		3.4
(-2.9) 		7
(-1.5) 		16
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 0.7
(-1)
A 		1.3
(3)
A 		0.4
(-3)
A 		1.3
(3)
A 		1
B 0.5
(-3)
A 		0.5
(-3)
A 		0.8
(1.5)
A 		0.9
(3)
A 		0
C 2.9
(-0.3)
A 		0.9
(-3)
A 		1.9
(-1.7)
A 		5.4
(3)
A 		5
avr 1.4
(-1.1) 		0.9
(-3) 		1
(-2.6) 		2.5
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 5.9
(3)
A 		1.1
(-2.3)
A 		1
(-2.4)
A 		1.3
(-2.1)
A 		0.5
(-3)
A 		1.2
(-2.2)
A 		5
B 0.9
(-2.1)
A 		1.5
(-0.8)
A 		0.5
(-3)
A 		1.1
(-1.7)
A 		2.4
(1.2)
A 		3.2
(3)
A 		3
C 0.6
(-3)
A 		0.9
(-2.8)
A 		0.7
(-2.9)
A 		9.5
(3)
A 		0.8
(-2.9)
A 		2.6
(-1.7)
A 		9
avr 2.5
(0.3) 		1.2
(-2.1) 		0.7
(-3) 		4
(3) 		1.2
(-2.1) 		2.3
(-0.1) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188736_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5351
Sequence Type Exemplar sequence
Representative Public ID F18A12.6 NCBI
Target Description F18A12.6 /REP_DB=WormBase Gene ID /WP=CE09459 /TR=O16790 /GB=AAB66078.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=peptidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062131(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062131 NCBI Caenorhabditis elegans F18A12.6 (peptidase) mRNA, complete cds. 11/11 None
SNAP00000014573 ENSEMBL cdna:SNAP chromosome:CEL140:II:3399792:3402049:-1 11/11 None
GENEFINDER00000014589 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3395853:3402049:-1 11/11 None
F18A12.6 ENSEMBL cdna:known chromosome:CEL140:II:3399792:3403315:-1 gene:F18A12.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3399790-3402885(-) 100.0 100.0
Public Domain and Genome References
Gene Title peptidase
Ensembl F18A12.6
Entrez Gene 173684 Entrez gene
SwissProt O16790 EMBL-EBI
WormBase CE09459 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB66078 Hypothetical protein F18A12.6 [Caenorhabditis elegans] ref|NP_494532.1| F18A12.6 [Caenorhabditis elegans] 0.0
blast AAB66077 Hypothetical protein F18A12.5 [Caenorhabditis elegans] ref|NP_494531.2| F18A12.5 [Caenorhabditis elegans] 0.0
blast AAB66123 Hypothetical protein T06D4.4 [Caenorhabditis elegans] ref|NP_494530.1| T06D4.4 [Caenorhabditis elegans] 0.0
blast AAL02468 Hypothetical protein C49D10.10 [Caenorhabditis elegans] ref|NP_494679.1| C49D10.10 [Caenorhabditis elegans] 1.0E-146
blast AAB66076 Hypothetical protein F18A12.4 [Caenorhabditis elegans] ref|NP_494533.2| F18A12.4 [Caenorhabditis elegans] 1.0E-146
Protein Domains
Database ID Description E-Value
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 6.7E-66
Pfam IPR008753 EMBL-EBI Peptidase M13 5.5E-5
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 5.2E-62
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.3E-29
Pfam IPR008753 EMBL-EBI Peptidase M13 7.4E-5
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 6.7E-66
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494532.1 1 13-35
F18A12.6 1 13-35
Sequence
Target Sequence 
>C. ELEGANS:188736_S_AT
gatccattcttcgattcgacgtatcccgaatatgtgaacctcatctttacgggattcctc
attggacacgagtttggtcactctattgatccaaaaatactgagaagagatggatggtat
aaaaccgaagatatgactgaatatgggaaacgagctcaatgtctgatagatcaatacgat
aactatgatgatccggatcacggaaaacagatgaatggaacatattgtatcggtgaaatt
gttggtgattgggttggacgtgatgtgacctggagggcttttaagaaaatggatctatcg
aaaatgcagaagctgattggatttgaggataagaatttggaccagctttattttagaatt
cagtctctgttcttctgtggtcctcgcagtatgaagagtttggagcagctcctcagtgat
ccccacccaactgaagttttccgtgtcaatggaatctactcaaatatgccacaatttgcg
aaggcgttcaattgtccgattgggtcgcctat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCCATTCTTCGATTCGACGTATC 2 417 1420 Antisense
CGACGTATCCCGAATATGTGAACCT 213 249 1436 Antisense
GTGAACCTCATCTTTACGGGATTCC 600 479 1453 Antisense
ACGGGATTCCTCATTGGACACGAGT 594 91 1468 Antisense
GAGTTTGGTCACTCTATTGATCCAA 499 397 1489 Antisense
GGACGTGATGTGACCTGGAGGGCTT 216 523 1675 Antisense
GAATTCAGTCTCTGTTCTTCTGTGG 174 325 1775 Antisense
TTCTTCTGTGGTCCTCGCAGTATGA 192 691 1789 Antisense
CACCCAACTGAAGTTTTCCGTGTCA 182 201 1843 Antisense
GGAATCTACTCAAATATGCCACAAT 486 531 1870 Antisense
TCAATTGTCCGATTGGGTCGCCTAT 653 633 1907 Antisense
Sequence Source Affymetrix Proprietary Database