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[188725_s_at] Elongation factor 1-alpha

Description F31E3.5 /REP_DB=WormBase Gene ID /WP=CE01270 /GEN=eft-3 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Elongation factor 1-alpha
Cluster 6grp_highest_10_10 > 25      6grp_high1_10_10 > 30      4grp_highest_8_8 > 14      4grp_high_8_8 > 22      6grp_highest_nonorm > 09      6grp_total > 52      4grp_total > 09      6grp_highest_sub > 03      6grp_cgc > 92      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24080
1 2 3 4 range
A 28421.5
(-3)
P 		34696.9
(-1.3)
P 		33000.7
(-1.8)
P 		50565.1
(3)
P 		22144
B 29721.1
(-3)
P 		30773.9
(-2.2)
P 		34848.4
(0.8)
P 		37742.7
(3)
P 		8022
C 26484.9
(-3)
P 		30342.3
(-2)
P 		26916.9
(-2.9)
P 		48880.2
(3)
P 		22395
avr 28209.2
(-3) 		31937.7
(-1.7) 		31588.7
(-1.8) 		45729.3
(3) 		17520

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10513
1 2 3 4 5 6 range
A 30350.5
(2.6)
P 		26425
(-1.2)
P 		30718.9
(3)
P 		26504.8
(-1.1)
P 		24528
(-3)
P 		29204
(1.5)
P 		6191
B 25752.7
(-1)
P 		26449.1
(-0.6)
P 		32675.9
(3)
P 		25610.1
(-1)
P 		22163.4
(-3)
P 		22793.4
(-2.6)
P 		10513
C 23299.6
(-3)
P 		27889.8
(1.3)
P 		28236.3
(1.7)
P 		29647.4
(3)
P 		25137.5
(-1.3)
P 		25378.2
(-1)
P 		6348
avr 26467.6
(-0.7) 		26921.3
(-0.3) 		30543.7
(3) 		27254.1
(0) 		23943
(-3) 		25791.9
(-1.3) 		6601
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2947
1 2 3 4 range
A 10348.3
(3)
P 		7531.8
(-2.7)
P 		8627
(-0.5)
P 		7401.7
(-3)
P 		2947
B 9477.8
(3)
P 		7600.4
(-3)
P 		8075.2
(-1.5)
P 		8043
(-1.6)
P 		1877
C 8767.3
(-0.2)
P 		8723.6
(-0.5)
P 		9381.4
(3)
P 		8242
(-3)
P 		1139
avr 9531.1
(3) 		7951.9
(-2.8) 		8694.5
(-0.1) 		7895.6
(-3) 		1636

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3803
1 2 3 4 5 6 range
A 5594.9
(-3)
P 		9398
(3)
P 		6689.9
(-1.3)
P 		6722.2
(-1.2)
P 		7401.5
(-0.1)
P 		7110.3
(-0.6)
P 		3803
B 8960.7
(2.9)
P 		8803.8
(2.5)
P 		7934.7
(0.1)
P 		6783.2
(-3)
P 		9004.7
(3)
P 		8499.5
(1.6)
P 		2222
C 8636.1
(3)
P 		8510
(2.7)
P 		7186.6
(-0.3)
P 		5962.1
(-3)
P 		8134.1
(1.9)
P 		8495.6
(2.7)
P 		2674
avr 7730.6
(0.1) 		8903.9
(3) 		7270.4
(-1.1) 		6489.2
(-3) 		8180.1
(1.2) 		8035.1
(0.8) 		2415
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188725_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6297
Sequence Type Exemplar sequence
Representative Public ID F31E3.5 NCBI
Target Description F31E3.5 /REP_DB=WormBase Gene ID /WP=CE01270 /GEN=eft-3 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Elongation factor 1-alpha
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066119(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066119 NCBI Caenorhabditis elegans Elongation FacTor family member (eft-3) (eft-3) mRNA, complete cds. 11/11 None
SNAP00000009455 ENSEMBL cdna:SNAP chromosome:CEL140:III:6970070:6971611:-1 11/11 None
GENEFINDER00000009470 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6970070:6971611:-1 11/11 None
F31E3.5.2 ENSEMBL cdna:known chromosome:CEL140:III:6969564:6971656:-1 gene:F31E3.5 11/11 None
F31E3.5.1 ENSEMBL cdna:known chromosome:CEL140:III:6969564:6971643:-1 gene:F31E3.5 11/11 None
F31E3.5.8 ENSEMBL cdna:known chromosome:CEL140:III:6969564:6971649:-1 gene:F31E3.5 11/11 None
F31E3.5.6 ENSEMBL cdna:known chromosome:CEL140:III:6969856:6971643:-1 gene:F31E3.5 11/11 None
F31E3.5.3 ENSEMBL cdna:known chromosome:CEL140:III:6969957:6971645:-1 gene:F31E3.5 11/11 None
F31E3.5.7 ENSEMBL cdna:known chromosome:CEL140:III:6969957:6972071:-1 gene:F31E3.5 11/11 None
F31E3.5.4 ENSEMBL cdna:known chromosome:CEL140:III:6969957:6971648:-1 gene:F31E3.5 11/11 None
F31E3.5.5 ENSEMBL cdna:known chromosome:CEL140:III:6969957:6972071:-1 gene:F31E3.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7823614-7825205(+) 93.97 93.97
chrIII:6970067-6971609(-) 100.0 100.0
Public Domain and Genome References
Gene Title Elongation factor 1-alpha
Gene Symbol eft-3
Ensembl F31E3.5
Entrez Gene 175975 Entrez gene
SwissProt P53013 EMBL-EBI
WormBase CE01270 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:15992_S_AT elongation factor 1-alpha / EF-1-alpha at
ATH1-121501:247644_S_AT elongation factor 1-alpha / EF-1-alpha at
CANINE:1604370_S_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE:1582740_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFA.1276.1.S1_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFA.1276.1.S1_X_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFA.16611.1.S1_X_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFA.853.1.S1_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.10241.1.S1_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.12630.1.S1_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.12788.1.S1_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.15052.1.S1_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.15179.1.S1_S_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.15179.1.S1_X_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.15189.1.S1_X_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.19254.1.S1_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.21554.1.S1_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.21951.1.S1_S_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.21951.1.S1_X_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.22536.1.S1_X_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.25079.1.S1_X_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.26727.1.S1_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.27311.1.S1_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.27311.1.S1_X_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.28996.1.S1_X_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.4045.1.S1_X_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.5542.1.S1_X_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
CANINE_2:CFAAFFX.714.1.S1_X_AT similar to eukaryotic translation elongation factor 1 alpha 1 cfa
DROSOPHILA_2:1624989_S_AT Elongation factor 1100E dm
DROSGENOME1:143142_AT Elongation factor 1100E dm
CHICKEN:GGA.4053.1.S1_AT eukaryotic translation elongation factor 1 alpha 2 gga
HG-U133_PLUS_2:204540_AT eukaryotic translation elongation factor 1 alpha 2 hs
HG-U133A:204540_AT eukaryotic translation elongation factor 1 alpha 2 hs
HG-FOCUS:204540_AT eukaryotic translation elongation factor 1 alpha 2 hs
HG-U133A_2:204540_AT eukaryotic translation elongation factor 1 alpha 2 hs
HUGENEFL:X70940_S_AT eukaryotic translation elongation factor 1 alpha 2 hs
U133_X3P:G4503474_3P_X_AT eukaryotic translation elongation factor 1 alpha 2 hs
U133_X3P:G4503474_3P_S_AT eukaryotic translation elongation factor 1 alpha 2 hs
U133_X3P:G4503474_3P_AT eukaryotic translation elongation factor 1 alpha 2 hs
U133_X3P:204540_3P_AT eukaryotic translation elongation factor 1 alpha 2 hs
HG-U95AV2:35174_I_AT eukaryotic translation elongation factor 1 alpha 2 hs
HG-U95AV2:35175_F_AT eukaryotic translation elongation factor 1 alpha 2 hs
MU11KSUBA:L26479_S_AT eukaryotic translation elongation factor 1 alpha 2 mm
MU11KSUBB:MSA.8297.0_F_AT eukaryotic translation elongation factor 1 alpha 2 mm
MG-U74AV2:94429_AT eukaryotic translation elongation factor 1 alpha 2 mm
MOE430A:1418062_AT eukaryotic translation elongation factor 1 alpha 2 mm
MOUSE430A_2:1418062_AT eukaryotic translation elongation factor 1 alpha 2 mm
MOUSE430_2:1418062_AT eukaryotic translation elongation factor 1 alpha 2 mm
MG-U74BV2:107332_AT Eukaryotic translation elongation factor 1 alpha 2, mRNA (cDNA clone MGC:25318 IMAGE:4504385) mm
MOE430B:1455690_AT Eukaryotic translation elongation factor 1 alpha 2, mRNA (cDNA clone MGC:25318 IMAGE:4504385) mm
MOUSE430_2:1455690_AT Eukaryotic translation elongation factor 1 alpha 2, mRNA (cDNA clone MGC:25318 IMAGE:4504385) mm
RG-U34A:M62752_AT statin-like rn
RAE230A:1367799_AT statin-like rn
RAT230_2:1367799_AT statin-like rn
RAE230B:1395478_AT statin-like rn
RAT230_2:1395478_AT statin-like rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
6414 translational elongation inferred from electronic annotation QuickGO AmiGO
43037 translation inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3746 translation elongation factor activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
8135 translation factor activity, nucleic acid binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA81688 Elongation factor protein 3 [Caenorhabditis elegans] gb|AAA96068.1| Elongation factor protein 4, isoform a [Caenorhabditis elegans] ref|NP_509323.1| Elongation FacTor family member (eft-4) [Caenorhabditis elegans] ref|NP_498520.1| Elongation FacTor family member (eft-3) [Caenorhabditis elegans] sp|P53013|EF1A_CAEEL Elongation factor 1-alpha (EF-1-alpha) 0.0
blast CAE70307 Hypothetical protein CBG16828 [Caenorhabditis briggsae] emb|CAE70057.1| Hypothetical protein CBG16491 [Caenorhabditis briggsae] emb|CAE56763.1| Hypothetical protein CBG24566 [Caenorhabditis briggsae] 0.0
blast P27592 Elongation factor 1-alpha (EF-1-alpha) gb|AAA29416.1| elongation factor 0.0
Protein Domains
Database ID Description E-Value
scop a.4.5.Transcription factor MotA, activation domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Transcription factor MotA, activation domain 1.20000004768372
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 2.1E-112
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 1.3E-25
Pfam IPR004160 EMBL-EBI Elongation factor Tu, C-terminal 6.4E-57
Sequence
Target Sequence 
>C. ELEGANS:188725_S_AT
cggaactgttccagtcggacgtgttgagaccggaatcatcaagccaggaatggtcgttac
cttcgctccacaaaacgtcaccactgaagtcaagtccgttgagatgcatcacgaatctct
tccagaggccgtcccaggagacaacgttggattcaacgtcaagaacgtctccgtcaagga
tattcgtcgtggatccgtctgctccgactccaagcaagacccagccaaggaggctcgcac
cttccacgcccaggtcatcatcatgaaccatccaggacagatctccaacggatacactcc
agttctcgattgccacaccgctcacatcgcctgcaagttcaacgagcttaaggagaaggt
tgaccgtcgtaccggtaagaaggttgaggacttcccaaagttcctcaagtctggagatgc
tggaatcgtcgagctcatcccaaccaagccactttgtgttgaatccttcaccgactacgc
tccactcggacgtttcgccgttcgtgacatgagacaaaccgtcgctgtcggagttatcaa
gtccgttgagaagtctgatggatcctctggaaaggtcaccaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGGAACTGTTCCAGTCGGACGTGTT 68 241 789 Antisense
CTCCACAAAACGTCACCACTGAAGT 186 235 854 Antisense
GTCAAGTCCGTTGAGATGCATCACG 302 463 877 Antisense
GCATCACGAATCTCTTCCAGAGGCC 520 309 894 Antisense
GAACGTCTCCGTCAAGGATATTCGT 298 347 951 Antisense
GCAAGACCCAGCCAAGGAGGCTCGC 457 321 1002 Antisense
CGCCCAGGTCATCATCATGAACCAT 306 257 1035 Antisense
GACAGATCTCCAACGGATACACTCC 252 367 1064 Antisense
TCAAGTCCGTTGAGAAGTCTGATGG 316 633 1325 Antisense
GAGAAGTCTGATGGATCCTCTGGAA 447 391 1336 Antisense
TGGATCCTCTGGAAAGGTCACCAAG 427 549 1347 Antisense
Sequence Source Affymetrix Proprietary Database