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[188724_s_at]

Description F47F6.5 /REP_DB=WormBase Gene ID /WP=CE10712 /TR=P91312 /GB=AAC71117.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=proteinase
Cluster 6grp_total > 91      4grp_total > 32      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 1.4
(-3)
A 		4.5
(-1.3)
A 		12.4
(3)
A 		12
(2.8)
A 		11
B 13.8
(-1)
A 		3.1
(-3)
A 		21.7
(0.5)
A 		34.7
(3)
A 		32
C 3.6
(-2.2)
A 		12
(1.1)
A 		17
(3)
A 		1.6
(-3)
A 		15
avr 6.3
(-3) 		6.5
(-2.9) 		17
(3) 		16.1
(2.5) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 1.5
(-3)
A 		30.6
(3)
A 		3.7
(-2.5)
A 		30.5
(3)
A 		1.4
(-3)
A 		1.7
(-2.9)
A 		29
B 3.8
(-2.4)
A 		4.3
(-2.3)
A 		1.3
(-3)
A 		16.8
(0.5)
A 		28
(3)
A 		2.5
(-2.7)
A 		27
C 5.4
(-0.8)
A 		1.7
(-2.8)
A 		12.4
(3)
A 		2.4
(-2.5)
A 		12.3
(2.9)
A 		1.4
(-3)
A 		11
avr 3.6
(-2.3) 		12.2
(1.2) 		5.8
(-1.4) 		16.6
(3) 		13.9
(1.9) 		1.9
(-3) 		15
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 0.5
(-3)
A 		1
(-1.9)
A 		3.3
(3)
A 		1.8
(-0.2)
A 		3
B 4.4
(0.3)
A 		0.8
(-3)
A 		5
(0.8)
A 		7.4
(3)
A 		7
C 1.2
(-2)
A 		3.4
(0.3)
A 		5.9
(3)
A 		0.3
(-3)
A 		6
avr 2
(-2.4) 		1.7
(-3) 		4.7
(3) 		3.2
(0) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 0.3
(-3)
A 		10.9
(3)
A 		0.8
(-2.7)
A 		7.7
(1.2)
A 		0.4
(-2.9)
A 		0.4
(-2.9)
A 		11
B 1.3
(-2.5)
A 		1.4
(-2.4)
A 		0.3
(-3)
A 		4.4
(-0.8)
A 		11.4
(3)
A 		0.9
(-2.7)
A 		11
C 2
(-0.4)
A 		0.5
(-3)
A 		3.2
(1.6)
A 		0.5
(-3)
A 		4
(3)
A 		0.5
(-3)
A 		4
avr 1.2
(-2.2) 		4.3
(1.7) 		1.4
(-2) 		4.2
(1.6) 		5.3
(3) 		0.6
(-3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188724_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4400
Sequence Type Exemplar sequence
Representative Public ID F47F6.5 NCBI
Target Description F47F6.5 /REP_DB=WormBase Gene ID /WP=CE10712 /TR=P91312 /GB=AAC71117.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=proteinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027008(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027008 NCBI Caenorhabditis elegans F47F6.9 (F47F6.9) mRNA, complete cds. 11/11 None
SNAP00000004730 ENSEMBL cdna:SNAP chromosome:CEL140:II:1260066:1260697:1 9/11 None
GENEFINDER00000004737 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1255598:1263004:1 10/11 None
F47F6.9 ENSEMBL cdna:known chromosome:CEL140:II:1259371:1260697:1 gene:F47F6.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1255596-1260696(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F47F6.9
Entrez Gene 3565584 Entrez gene
SwissProt Q6F6K4 EMBL-EBI
WormBase CE36854 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4198 calpain activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAT48617 Hypothetical protein F47F6.9 [Caenorhabditis elegans] ref|NP_001022179.1| F47F6.9 [Caenorhabditis elegans] 0.0
blast C88040 protein F47F6.5 [imported] - Caenorhabditis elegans 0.0
blast AAT48616 Hypothetical protein F47F6.5 [Caenorhabditis elegans] ref|NP_493933.2| F47F6.5 [Caenorhabditis elegans] 0.0
blast AAT48617 Hypothetical protein F47F6.9 [Caenorhabditis elegans] ref|NP_001022179.1| F47F6.9 [Caenorhabditis elegans] 1.0E-164
blast AAT48617 Hypothetical protein F47F6.9 [Caenorhabditis elegans] ref|NP_001022179.1| F47F6.9 [Caenorhabditis elegans] 1.0E-101
blast C88040 protein F47F6.5 [imported] - Caenorhabditis elegans 1.0E-101
blast CAE57509 Hypothetical protein CBG00483 [Caenorhabditis briggsae] 4.0E-38
Protein Domains
Database ID Description E-Value
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 3.5E-8
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.3E-5
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.3E-5
Sequence
Target Sequence 
>C. ELEGANS:188724_S_AT
atcagtccaaatcacgggcacgggtacactgaaatggggtcatttaccgagattcgatgg
caagttccaattggaaagaatatactgataaattatgattcagaaaaatgcctttgtggg
aatattttcgactatgttctctcaacgactagcatccattgggtggacttgcatattttg
tctcgattcttttcatgctttgaaatttgtcattatcgtgaaggctggaaagaaatccgt
tttcgaaagactttggttccaaagaacggcaagacagaaattttaaaactatttctcaaa
acaagatgcgagctggttattgaggttatcagacgaaaagaagcgctaagattagaagcg
gaacaattgagaaataggtgtatactgttctgcattgtttattctgcaactgcggacaat
aaatgtgataagattctgatgattactcatgcatatgacttgaatttggaattaacaccc
ggcacacttgatcctggaacatat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCAGTCCAAATCACGGGCACGGGT 465 27 2848 Antisense
GGCACGGGTACACTGAAATGGGGTC 704 521 2864 Antisense
TACCGAGATTCGATGGCAAGTTCCA 690 645 2892 Antisense
TATTTTCGACTATGTTCTCTCAACG 83 663 2970 Antisense
TCTCTCAACGACTAGCATCCATTGG 30 613 2985 Antisense
ACTTGCATATTTTGTCTCGATTCTT 78 105 3014 Antisense
GTCTCGATTCTTTTCATGCTTTGAA 468 469 3027 Antisense
GATGCGAGCTGGTTATTGAGGTTAT 497 413 3152 Antisense
GCATTGTTTATTCTGCAACTGCGGA 523 305 3239 Antisense
TTTGGAATTAACACCCGGCACACTT 703 663 3312 Antisense
CGGCACACTTGATCCTGGAACATAT 309 243 3327 Antisense
Sequence Source Affymetrix Proprietary Database