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[188723_at] peptidase

Description Y95B8A.1 /REP_DB=WormBase Gene ID /WP=CE24689 /TR=Q9N2V2 /GB=AAF60901.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=peptidase
Cluster 6grp_high1_10_10 > 90      6grp_total > 92      4grp_total > 57      6grp_cgc > 64      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 476
1 2 3 4 range
A 43.8
(-2.5)
A 		362.6
(1.9)
P 		439.2
(3)
P 		4
(-3)
A 		435
B 58.8
(-2.4)
A 		413.6
(2.9)
P 		422.2
(3)
P 		19.4
(-3)
A 		403
C 70.9
(-2.2)
A 		343.2
(1.3)
P 		479.9
(3)
P 		10.6
(-3)
A 		469
avr 57.8
(-2.4) 		373.1
(2) 		447.1
(3) 		11.3
(-3) 		436

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 191
1 2 3 4 5 6 range
A 169.3
(-2)
A 		303.4
(3)
P 		187.2
(-1.3)
P 		145
(-2.9)
A 		286.2
(2.4)
P 		141.4
(-3)
P 		162
B 170.7
(-1.5)
P 		250.3
(1.6)
P 		205.8
(-0.1)
M 		185.7
(-0.9)
P 		288.1
(3)
P 		130.4
(-3)
P 		158
C 159.8
(-1.7)
M 		260.6
(1.5)
P 		243.3
(1)
M 		219.1
(0.2)
P 		307.9
(3)
P 		116.9
(-3)
P 		191
avr 166.6
(-1.7) 		271.4
(2.2) 		212.1
(0) 		183.3
(-1) 		294.1
(3) 		129.6
(-3) 		165
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 167
1 2 3 4 range
A 16
(-2.2)
A 		78.7
(1.1)
P 		114.8
(3)
P 		0.6
(-3)
A 		114
B 18.8
(-2.1)
A 		102.2
(3)
P 		97.8
(2.7)
P 		4.1
(-3)
A 		98
C 23.5
(-2.2)
A 		98.7
(0.5)
P 		167.3
(3)
P 		1.8
(-3)
A 		166
avr 19.4
(-2.2) 		93.2
(1.4) 		126.6
(3) 		2.2
(-3) 		124

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 31.2
(-3)
A 		107.9
(3)
P 		40.8
(-2.2)
P 		36.8
(-2.6)
A 		86.3
(1.3)
P 		34.4
(-2.7)
P 		77
B 59.4
(-2.1)
P 		83.3
(0)
P 		50
(-2.9)
M 		49.2
(-2.9)
P 		117.1
(3)
P 		48.6
(-3)
P 		69
C 59.2
(-1)
M 		79.5
(1)
P 		61.9
(-0.7)
M 		44.1
(-2.5)
P 		99.6
(3)
P 		39.1
(-3)
P 		61
avr 49.9
(-2.1) 		90.2
(1.9) 		50.9
(-2) 		43.4
(-2.7) 		101
(3) 		40.7
(-3) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188723_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.30
Sequence Type Exemplar sequence
Representative Public ID Y95B8A.1 NCBI
Target Description Y95B8A.1 /REP_DB=WormBase Gene ID /WP=CE24689 /TR=Q9N2V2 /GB=AAF60901.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=peptidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058394(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058394 NCBI Caenorhabditis elegans Y95B8A.1 (peptidase) mRNA, complete cds. 11/11 None
SNAP00000034260 ENSEMBL cdna:SNAP chromosome:CEL140:I:938627:947545:1 10/11 None
Y95B8A.1 ENSEMBL cdna:known chromosome:CEL140:I:942414:947678:1 gene:Y95B8A.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000034289 3/11 Cross Hyb Matching Probes None
GENEFINDER00000034292 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:942258-947678(+) 100.0 100.0
Public Domain and Genome References
Gene Title peptidase
Gene Symbol nas-30
Ensembl Y95B8A.1
Entrez Gene 190803 Entrez gene
SwissProt Q9N2V2 EMBL-EBI
WormBase CE36770 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
8533 astacin activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF60901 Nematode astacin protease protein 30 [Caenorhabditis elegans] ref|NP_490795.2| Y95B8A.1 [Caenorhabditis elegans] 0.0
blast Q9N2V2 Zinc metalloproteinase nas-30 precursor (Nematode astacin 30) 0.0
blast CAE61297 Hypothetical protein CBG05121 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 3.3E-99
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 3.3E-99
Sequence
Target Sequence 
>C. ELEGANS:188723_AT
ctcaccgagctcaggtaccagtgtgcaccggcgtgtaaggcctacatcgagatcaagcat
aataccgacttccagcagaccggattcagagtttgctgcttcaacaaaacatatgacgtc
atctccgaccaatctgaagccctgatcctctccaacgcgaacatcgtggactacgaggtc
tcctacaagctgcaatggattcaaggtaccccacccccccccccttttctatccatagag
cgcactttcttccagacaacggaaaagctcttccaccaccgaaacccacgtcgacctggg
tgcccggaaaagagaatcgaccattccgcggagtggagaactcgggcggcaccatcgaaa
agttcatcctgcaagcgatcccaaagatccgagactcgcatcggccactggagagtatta
ctagtattgttgcggaatatggtctcgcaacgctacttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCACCGAGCTCAGGTACCAGTGTG 156 229 1639 Antisense
TGTGCACCGGCGTGTAAGGCCTACA 703 463 1660 Antisense
AATACCGACTTCCAGCAGACCGGAT 200 173 1699 Antisense
GACCGGATTCAGAGTTTGCTGCTTC 249 383 1716 Antisense
TCCTCTCCAACGCGAACATCGTGGA 600 591 1784 Antisense
CTACGAGGTCTCCTACAAGCTGCAA 558 225 1809 Antisense
GCAATGGATTCAAGGTACCCCACCC 429 321 1830 Antisense
AATCGACCATTCCGCGGAGTGGAGA 327 167 1953 Antisense
ACTCGGGCGGCACCATCGAAAAGTT 520 95 1978 Antisense
GAAAAGTTCATCCTGCAAGCGATCC 2 355 1995 Antisense
GAATATGGTCTCGCAACGCTACTTG 700 325 2073 Antisense
Sequence Source Affymetrix Proprietary Database