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[188722_at] peptidase

Description F18A12.1 /REP_DB=WormBase Gene ID /WP=CE09454 /TR=O16795 /GB=AAB66080.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=peptidase
Cluster 6grp_total > 78      4grp_total > 10      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 49.7
(-2.3)
A 		89.9
(3)
A 		79.7
(1.7)
A 		44.3
(-3)
A 		46
B 36
(1.4)
A 		18.3
(-3)
A 		34.5
(1.1)
A 		42.2
(3)
A 		24
C 41.5
(-0.2)
A 		25.6
(-1.7)
A 		11.4
(-3)
A 		75.7
(3)
A 		64
avr 42.4
(-2.8) 		44.6
(-1.7) 		41.9
(-3) 		54.1
(3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 81
1 2 3 4 5 6 range
A 10.1
(-3)
A 		15.2
(-2.4)
A 		50.3
(1.5)
A 		15.3
(-2.4)
A 		63.6
(3)
A 		62.3
(2.9)
A 		54
B 50.9
(0.7)
A 		10.9
(-2.6)
A 		5.5
(-3)
A 		46.2
(0.3)
A 		57.8
(1.3)
A 		79.1
(3)
A 		74
C 20.7
(-1.9)
A 		28.4
(-1.4)
A 		6.8
(-3)
A 		17
(-2.2)
A 		9.7
(-2.8)
A 		86
(3)
A 		79
avr 27.2
(-2.1) 		18.2
(-3) 		20.9
(-2.7) 		26.2
(-2.2) 		43.7
(-0.3) 		75.8
(3) 		58
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 18.1
(1.9)
A 		19.5
(2.5)
A 		20.8
(3)
A 		6.5
(-3)
A 		14
B 11.5
(3)
A 		4.5
(-3)
A 		8
(0)
A 		9
(0.9)
A 		7
C 13.7
(3)
A 		7.4
(-0.9)
A 		4
(-3)
A 		12.8
(2.4)
A 		10
avr 14.4
(3) 		10.5
(-1.7) 		10.9
(-1.2) 		9.4
(-3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 1.9
(-3)
A 		5.4
(-1.8)
A 		11
(0.2)
A 		3.9
(-2.3)
A 		19.2
(3)
A 		15.2
(1.6)
A 		17
B 17.7
(0.5)
A 		3.6
(-2.5)
A 		1.3
(-3)
A 		12.2
(-0.7)
A 		23.5
(1.7)
A 		29.5
(3)
A 		28
C 7.7
(-1.7)
A 		8.7
(-1.5)
A 		1.7
(-3)
A 		3.4
(-2.6)
A 		3.1
(-2.7)
A 		28.8
(3)
A 		27
avr 9.1
(-1.7) 		5.9
(-2.6) 		4.7
(-3) 		6.5
(-2.5) 		15.3
(0.2) 		24.5
(3) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188722_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4724
Sequence Type Exemplar sequence
Representative Public ID F18A12.1 NCBI
Target Description F18A12.1 /REP_DB=WormBase Gene ID /WP=CE09454 /TR=O16795 /GB=AAB66080.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=peptidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062136(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062136 NCBI Caenorhabditis elegans F18A12.1 (peptidase) mRNA, complete cds. 11/11 None
GENEFINDER00000014590 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3416833:3420714:1 11/11 None
F18A12.1 ENSEMBL cdna:known chromosome:CEL140:II:3416833:3420651:1 gene:F18A12.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3416831-3420650(+) 99.59 100.0
Public Domain and Genome References
Gene Title peptidase
Ensembl F18A12.1
Entrez Gene 184628 Entrez gene
SwissProt O16795 EMBL-EBI
WormBase CE09454 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB66080 Hypothetical protein F18A12.1 [Caenorhabditis elegans] ref|NP_494537.1| F18A12.1 [Caenorhabditis elegans] 0.0
blast AAL02468 Hypothetical protein C49D10.10 [Caenorhabditis elegans] ref|NP_494679.1| C49D10.10 [Caenorhabditis elegans] 1.0E-168
blast T31991 hypothetical protein C49D10.10 - Caenorhabditis elegans 1.0E-166
blast AAL02468 Hypothetical protein C49D10.10 [Caenorhabditis elegans] ref|NP_494679.1| C49D10.10 [Caenorhabditis elegans] 1.0E-166
blast T31991 hypothetical protein C49D10.10 - Caenorhabditis elegans 1.0E-164
Protein Domains
Database ID Description E-Value
Pfam IPR008753 EMBL-EBI Peptidase M13 3.4E-6
Pfam IPR008753 EMBL-EBI Peptidase M13 1.3E-6
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.2E-103
Pfam IPR008753 EMBL-EBI Peptidase M13 1.3E-6
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.2E-103
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494537.1 1 7-29
F18A12.1 1 7-29
GENEFINDER00000014590 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:188722_AT
ccggttctcatgacgaacgcgttttatactgatcgaaatcatttgtctttccttgtcggg
tacatggatgatccgcttttcgacgccacctacccaaaatacgtaaatattgctctaacc
ggaaacattttggctcacgaaactagtcatggattcgatacagcaggtataaagagggac
gaaaacggagaagtcagaagttggatgaagccggaggatttgaaggagtatgggaagaaa
actcagtgcctggtagatcaatacaatgagtatgatgatccggtttttggcaaaaatctc
aacggaactacaactctcaacgagatggtggccgacatgattggagttacagttgcttgg
aaggtctttgaaaaactcgattcatcaaaagagcaaaaagtcattggatttgaaaatgaa
aatattgataagctctatttcagagcggctgctttggatttctgctcgttgcccccaaca
acaagcctggtagaagagctcaccagagtacacccgactggaaatttccgtgtcaatggg
atcttttccaatatgccacaatttgcgacagcattcaactgcccagaaggatcacctat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCGGTTCTCATGACGAACGCGTTTT 458 263 1564 Antisense
ATTTGTCTTTCCTTGTCGGGTACAT 22 15 1604 Antisense
GTACATGGATGATCCGCTTTTCGAC 689 459 1623 Antisense
TAACCGGAAACATTTTGGCTCACGA 91 633 1679 Antisense
TCAACGGAACTACAACTCTCAACGA 221 625 1862 Antisense
GAGCGGCTGCTTTGGATTTCTGCTC 368 387 2006 Antisense
GCCCCCAACAACAAGCCTGGTAGAA 615 279 2034 Antisense
AGAAGAGCTCACCAGAGTACACCCG 4 75 2055 Antisense
GAGTACACCCGACTGGAAATTTCCG 252 311 2069 Antisense
TTTGCGACAGCATTCAACTGCCCAG 298 669 2125 Antisense
TCAACTGCCCAGAAGGATCACCTAT 241 631 2138 Antisense
Sequence Source Affymetrix Proprietary Database