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[188721_at] MIP protein

Description C32C4.2 /REP_DB=WormBase Gene ID /WP=CE05345 /TR=Q18352 /GB=CAA98110.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=MIP protein
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 89      4grp_high_8_8 > 13      6grp_highest_nonorm > 91      6grp_total > 95      4grp_total > 02      6grp_cgc > 78      6grp_high1_sub > Cu      6grp_cgc_sub > In      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 504
1 2 3 4 range
A 85
(-3)
P 		105.3
(-2.7)
P 		83
(-3)
M 		554.1
(3)
P 		471
B 107.5
(-2.5)
P 		69.9
(-3)
P 		81.6
(-2.9)
P 		544.9
(3)
P 		475
C 118.1
(-2.2)
P 		98.1
(-2.4)
P 		50.4
(-3)
P 		533.7
(3)
P 		483
avr 103.5
(-2.6) 		91.1
(-2.8) 		71.7
(-3) 		544.2
(3) 		473

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 123
1 2 3 4 5 6 range
A 192.1
(1)
P 		134.3
(-3)
P 		137
(-2.8)
P 		207.7
(2)
P 		187.5
(0.7)
P 		221.6
(3)
P 		87
B 219.6
(1.4)
P 		186
(-0.2)
P 		128.3
(-3)
P 		204.3
(0.7)
P 		251.7
(3)
P 		243
(2.6)
P 		123
C 157.9
(-2.3)
P 		212.7
(1)
P 		147.1
(-3)
P 		242.4
(2.9)
P 		221.4
(1.6)
P 		244.4
(3)
P 		97
avr 189.9
(0.2) 		177.7
(-0.6) 		137.5
(-3) 		218.1
(1.9) 		220.2
(2) 		236.3
(3) 		99
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 31
(-2.1)
P 		22.9
(-2.9)
P 		21.7
(-3)
M 		81.1
(3)
P 		59
B 34.3
(-2)
P 		17.3
(-3)
P 		18.9
(-2.9)
P 		116.1
(3)
P 		99
C 39.1
(-1.2)
P 		28.2
(-2.1)
P 		17.6
(-3)
P 		90
(3)
P 		72
avr 34.8
(-1.8) 		22.8
(-2.7) 		19.4
(-3) 		95.7
(3) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 35.4
(-1.7)
P 		47.8
(1)
P 		29.8
(-3)
P 		52.7
(2.1)
P 		56.6
(3)
P 		53.9
(2.4)
P 		27
B 76.4
(0.8)
P 		61.9
(-0.4)
P 		31.2
(-3)
P 		54.1
(-1.1)
P 		102.3
(3)
P 		90.6
(2)
P 		71
C 58.5
(-0.1)
P 		64.9
(0.7)
P 		37.4
(-3)
P 		48.7
(-1.5)
P 		71.6
(1.6)
P 		81.8
(3)
P 		44
avr 56.8
(0.3) 		58.2
(0.5) 		32.8
(-3) 		51.8
(-0.4) 		76.8
(3) 		75.4
(2.8) 		44
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188721_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13285
Sequence Type Exemplar sequence
Representative Public ID C32C4.2 NCBI
Target Description C32C4.2 /REP_DB=WormBase Gene ID /WP=CE05345 /TR=Q18352 /GB=CAA98110.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=MIP protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073326(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073326 NCBI Caenorhabditis elegans AQuaPorin or aquaglyceroporin related family member (aqp-6) (aqp-6) mRNA, complete cds. 11/11 None
SNAP00000003073 ENSEMBL cdna:SNAP chromosome:CEL140:V:10653586:10654405:1 11/11 None
GENEFINDER00000003078 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10650359:10654405:1 11/11 None
C32C4.2 ENSEMBL cdna:known chromosome:CEL140:V:10653318:10654405:1 gene:C32C4.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10653317-10654405(+) 100.0 100.0
Public Domain and Genome References
Gene Title MIP protein
Gene Symbol aqp-6
Ensembl C32C4.2
Entrez Gene 183121 Entrez gene
SwissProt Q18352 EMBL-EBI
WormBase CE05345 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1586935_AT similar to aquaporin 8 cfa
CANINE_2:CFA.11607.1.S1_AT similar to aquaporin 8 cfa
CANINE_2:CFAAFFX.26743.1.S1_AT similar to aquaporin 8 cfa
CANINE_2:CFAAFFX.26743.1.S1_S_AT similar to aquaporin 8 cfa
DROSOPHILA_2:1629642_A_AT dm
DROSGENOME1:152683_AT dm
CHICKEN:GGA.6178.1.S1_AT similar to Aquaporin 8 gga
CHICKEN:GGAAFFX.3722.1.S1_AT similar to Aquaporin 8 gga
HG-U133_PLUS_2:206784_AT aquaporin 8 hs
HG-U133A:206784_AT aquaporin 8 hs
HG-FOCUS:206784_AT aquaporin 8 hs
HG-U133A_2:206784_AT aquaporin 8 hs
U133_X3P:G4502188_3P_AT aquaporin 8 hs
HG-U95AV2:33651_AT aquaporin 8 hs
MU11KSUBA:AF018952_S_AT aquaporin 8 mm
MG-U74AV2:102200_AT aquaporin 8 mm
MOE430A:1417828_AT aquaporin 8 mm
MOUSE430A_2:1417828_AT aquaporin 8 mm
MOUSE430_2:1417828_AT aquaporin 8 mm
MU11KSUBB:MSA.29936.0_S_AT Aquaporin 8, mRNA (cDNA clone MGC:13894 IMAGE:4224850) mm
MU19KSUBB:TC34045_S_AT Aquaporin 8, mRNA (cDNA clone MGC:13894 IMAGE:4224850) mm
RG-U34A:AB005547_AT aquaporin 8 rn
RAE230A:1368316_AT aquaporin 8 rn
RAT230_2:1368316_AT aquaporin 8 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98110 Hypothetical protein C32C4.2 [Caenorhabditis elegans] ref|NP_505727.1| AQuaPorin or aquaglyceroporin related family member (aqp-6) [Caenorhabditis elegans] 1.0E-126
blast CAE64865 Hypothetical protein CBG09664 [Caenorhabditis briggsae] 1.0E-111
blast CAA98110 Hypothetical protein C32C4.2 [Caenorhabditis elegans] ref|NP_505727.1| AQuaPorin or aquaglyceroporin related family member (aqp-6) [Caenorhabditis elegans] 1.0E-109
blast CAE64865 Hypothetical protein CBG09664 [Caenorhabditis briggsae] 2.0E-95
blast CAA94779 Hypothetical protein F40F9.9 [Caenorhabditis elegans] emb|CAA94770.2| Hypothetical protein F40F9.9 [Caenorhabditis elegans] ref|NP_505512.3| AQuaPorin or aquaglyceroporin related family member (aqp-4) [Caenorhabditis elegans] 2.0E-54
blast CAA94779 Hypothetical protein F40F9.9 [Caenorhabditis elegans] emb|CAA94770.2| Hypothetical protein F40F9.9 [Caenorhabditis elegans] ref|NP_505512.3| AQuaPorin or aquaglyceroporin related family member (aqp-4) [Caenorhabditis elegans] 3.0E-54
Protein Domains
Database ID Description E-Value
Pfam IPR000425 EMBL-EBI Major intrinsic protein 1.4E-64
Pfam IPR000425 EMBL-EBI Major intrinsic protein 1.0E-57
Pfam IPR000425 EMBL-EBI Major intrinsic protein 1.4E-64
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505727.1 5 15-37,44-66,81-103,159-181,214-236
C32C4.2 5 15-37,44-66,81-103,159-181,214-236
SNAP00000003073 5 15-37,57-79,104-123,130-152,185-207
GENEFINDER00000003078 6 77-94,104-126,146-168,192-211,218-240,273-295
Sequence
Target Sequence 
>C. ELEGANS:188721_AT
atggagtacttcatgcagctttcgcccatggagtagctatttttgtacttgcggcaactt
ttggaggagtcagtggagctcatatcaatcctgcagtaacttttggaattgctcttgttg
gacgtatcagcccaattcatgccgtctgctatgtggtctctcaactacttggaagtgtct
tcggtgccctccttgttcggatttcactcccttataaaatgtataatgtcattagcgctg
gcgccaccctgtgtggaaaaggttacaactggcaagagggactaactgctgaaattgtga
caacttatattcttgtacaaacagttcttctttgtgcagtggatactgataaaaatagat
tggctccattggctattggattttcgttgataattgaaattcttgctgctggagctatct
ctggagcttcaatgaatcctgctcgttcttttggaccaaatattatgggacaagtgttct
tgaagcctgagcatcttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGAGTACTTCATGCAGCTTTCGC 597 51 128 Antisense
GAGCTCATATCAATCCTGCAGTAAC 682 387 203 Antisense
TGGACGTATCAGCCCAATTCATGCC 522 547 246 Antisense
AATTCATGCCGTCTGCTATGTGGTC 62 175 261 Antisense
CTATGTGGTCTCTCAACTACTTGGA 298 225 276 Antisense
TTGTTCGGATTTCACTCCCTTATAA 133 703 320 Antisense
ATAATGTCATTAGCGCTGGCGCCAC 483 25 350 Antisense
CAGTTCTTCTTTGTGCAGTGGATAC 672 205 449 Antisense
TCTTGCTGCTGGAGCTATCTCTGGA 625 617 528 Antisense
CAATGAATCCTGCTCGTTCTTTTGG 686 181 557 Antisense
GTGTTCTTGAAGCCTGAGCATCTTG 387 485 601 Antisense
Sequence Source Affymetrix Proprietary Database