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[188719_at] G-protein

Description Y95B8A.5 /REP_DB=WormBase Gene ID /WP=CE24692 /TR=Q9N2V6 /GB=AAF60904.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=G-protein
Cluster 6grp_total > 45      4grp_total > 11      6grp_cgc > 35      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 148
1 2 3 4 range
A 46.2
(-3)
A 		103.3
(-0.4)
A 		72.7
(-1.8)
A 		179.9
(3)
A 		134
B 97
(3)
A 		72
(-3)
A 		77.6
(-1.7)
A 		74.6
(-2.4)
A 		25
C 79.2
(-0.5)
A 		55.4
(-1.8)
A 		32
(-3)
A 		146.8
(3)
P 		115
avr 74.1
(-1.9) 		76.9
(-1.7) 		60.8
(-3) 		133.8
(3) 		73

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 76
1 2 3 4 5 6 range
A 67.3
(3)
A 		48.7
(1)
A 		54.6
(1.6)
A 		26.5
(-1.4)
A 		11.8
(-3)
A 		17.3
(-2.4)
A 		56
B 44.6
(1)
A 		51.2
(2)
A 		43.9
(0.9)
A 		18.4
(-2.7)
A 		16.6
(-3)
A 		58.4
(3)
A 		42
C 69.1
(0.9)
A 		38.8
(-2.4)
A 		87.9
(3)
A 		39.3
(-2.4)
A 		33.7
(-3)
A 		72
(1.2)
A 		54
avr 60.3
(2.7) 		46.2
(0.7) 		62.1
(3) 		28.1
(-1.9) 		20.7
(-3) 		49.2
(1.1) 		41
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 16.8
(-3)
A 		22.4
(0.5)
A 		19
(-1.6)
A 		26.3
(3)
A 		10
B 30.9
(3)
A 		17.8
(-2.2)
A 		18
(-2.2)
A 		15.9
(-3)
A 		15
C 26.2
(3)
A 		15.9
(-1.1)
A 		11.1
(-3)
A 		24.8
(2.4)
P 		15
avr 24.6
(3) 		18.7
(-1.1) 		16
(-3) 		22.3
(1.4) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 12.4
(0.9)
A 		17.3
(3)
A 		11.9
(0.6)
A 		6.7
(-1.6)
A 		3.6
(-3)
A 		4.2
(-2.7)
A 		14
B 15.5
(0.8)
A 		17
(1.3)
A 		10.7
(-0.9)
A 		4.9
(-3)
A 		6.7
(-2.4)
A 		21.8
(3)
A 		17
C 25.6
(3)
A 		11.9
(-1.6)
A 		22.4
(1.9)
A 		7.9
(-3)
A 		10.9
(-2)
A 		24.1
(2.5)
A 		18
avr 17.8
(3) 		15.4
(1.7) 		15
(1.5) 		6.5
(-3) 		7.1
(-2.7) 		16.7
(2.4) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188719_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10
Sequence Type Exemplar sequence
Representative Public ID Y95B8A.5 NCBI
Target Description Y95B8A.5 /REP_DB=WormBase Gene ID /WP=CE24692 /TR=Q9N2V6 /GB=AAF60904.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=G-protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058389(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058389 NCBI Caenorhabditis elegans Y95B8A.5 (G-protein) mRNA, complete cds. 11/11 None
GENEFINDER00000034290 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:897292:905855:1 11/11 None
Y95B8A.5 ENSEMBL cdna:known chromosome:CEL140:I:897292:905855:1 gene:Y95B8A.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000043041 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:897291-905855(+) 100.0 100.0
Public Domain and Genome References
Gene Title G-protein
Gene Symbol gpa-16
Ensembl Y95B8A.5
Entrez Gene 171675 Entrez gene
SwissProt Q9N2V6 EMBL-EBI
WormBase CE24692 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.3177.1.S1_AT similar to guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 cfa
CANINE_2:CFAAFFX.25135.1.S1_S_AT similar to guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 cfa
DROSOPHILA_2:1627491_AT G protein i subunit 65A dm
DROSGENOME1:142678_AT G protein i subunit 65A dm
CHICKEN:GGA.18910.1.S1_AT guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 gga
CHICKEN:GGA.18910.1.S1_S_AT guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 gga
CHICKEN:GGA.2167.1.S1_AT guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 gga
HG-U133_PLUS_2:209576_AT guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 hs
HG-U133A_2:209576_AT guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 hs
HG-U133A:209576_AT guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 hs
HG-FOCUS:209576_AT guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 hs
HU35KSUBB:RC_Z98492_AT guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 hs
HUGENEFL:M17219_AT guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 hs
U133_X3P:G4884070_3P_AT guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 hs
U133_X3P:HS.6975.1.A1_3P_AT guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 hs
HG-U133_PLUS_2:227692_AT guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 hs
HG-U133B:227692_AT guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 hs
HG-U95AV2:33809_AT guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 hs
HG-U95B:44001_AT guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 hs
HG-U95C:49517_AT Guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 hs
HG-U95D:78643_AT Guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 hs
MOUSE430A_2:1454959_S_AT guanine nucleotide binding protein, alpha inhibiting 1 mm
MOE430A:1454959_S_AT guanine nucleotide binding protein, alpha inhibiting 1 mm
MOUSE430_2:1454959_S_AT guanine nucleotide binding protein, alpha inhibiting 1 mm
MOUSE430A_2:1434440_AT guanine nucleotide binding protein, alpha inhibiting 1 mm
MOE430A:1434440_AT guanine nucleotide binding protein, alpha inhibiting 1 mm
MOUSE430_2:1434440_AT guanine nucleotide binding protein, alpha inhibiting 1 mm
MOUSE430A_2:1427510_AT guanine nucleotide binding protein, alpha inhibiting 1 mm
MOE430A:1427510_AT guanine nucleotide binding protein, alpha inhibiting 1 mm
MOUSE430_2:1427510_AT guanine nucleotide binding protein, alpha inhibiting 1 mm
MU11KSUBA:AA500440_AT guanine nucleotide binding protein, alpha inhibiting 1 mm
MU11KSUBB:MSA.16081.0_AT guanine nucleotide binding protein, alpha inhibiting 1 mm
MU11KSUBB:MSA.2735.0_S_AT guanine nucleotide binding protein, alpha inhibiting 1 mm
MU11KSUBB:MSA.10410.0_F_AT guanine nucleotide binding protein, alpha inhibiting 1 mm
MG-U74AV2:104412_AT guanine nucleotide binding protein, alpha inhibiting 1 mm
MG-U74AV2:97195_AT guanine nucleotide binding protein, alpha inhibiting 1 mm
MU19KSUBC:TC40705_AT guanine nucleotide binding protein, alpha inhibiting 1 mm
MG-U74BV2:106807_I_AT Guanine nucleotide binding protein, alpha inhibiting 1 (Gnai1), mRNA mm
MG-U74BV2:106808_R_AT Guanine nucleotide binding protein, alpha inhibiting 1 (Gnai1), mRNA mm
RG-U34A:M17527_AT guanine nucleotide binding protein, alpha inhibiting 1 rn
RAE230A:1387505_AT guanine nucleotide binding protein, alpha inhibiting 1 rn
RAT230_2:1387505_AT guanine nucleotide binding protein, alpha inhibiting 1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5834 heterotrimeric G-protein complex inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4871 signal transducer activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
3924 GTPase activity inferred from sequence similarity QuickGO AmiGO
5525 GTP binding inferred from sequence similarity QuickGO AmiGO
19003 GDP binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF60904 G protein, alpha subunit protein 16 [Caenorhabditis elegans] ref|NP_490790.1| Y95B8A.5 [Caenorhabditis elegans] gb|AAG32091.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|Q9N2V6|GPA16_CAEEL Guanine nucleotide-binding protein alpha-16 subunit 0.0
blast AAW02900 gpa-16 [Caenorhabditis briggsae] sp|Q60XS3|GPA16_CAEBR Guanine nucleotide-binding protein alpha-16 subunit emb|CAE71591.1| Hypothetical protein CBG18548 [Caenorhabditis briggsae] 1.0E-159
blast AAW02900 gpa-16 [Caenorhabditis briggsae] sp|Q60XS3|GPA16_CAEBR Guanine nucleotide-binding protein alpha-16 subunit emb|CAE71591.1| Hypothetical protein CBG18548 [Caenorhabditis briggsae] 1.0E-157
blast NP_037277 guanine nucleotide binding protein, alpha inhibiting 1 [Rattus norvegicus] gb|AAZ23809.1| guanine nucleotide binding protein alpha inhibiting 1 [Rattus norvegicus] gb|AAZ23808.1| guanine nucleotide binding protein alpha inhibiting 1 [Rattus norvegicus] sp|P10824|GNAI1_RAT Guanine nucleotide-binding protein G(i), alpha-1 subunit (Adenylate cyclase-inhibiting G alpha protein) gb|AAA40825.1| GTP-binding protein 1.0E-133
blast NP_037277 guanine nucleotide binding protein, alpha inhibiting 1 [Rattus norvegicus] gb|AAZ23809.1| guanine nucleotide binding protein alpha inhibiting 1 [Rattus norvegicus] gb|AAZ23808.1| guanine nucleotide binding protein alpha inhibiting 1 [Rattus norvegicus] sp|P10824|GNAI1_RAT Guanine nucleotide-binding protein G(i), alpha-1 subunit (Adenylate cyclase-inhibiting G alpha protein) gb|AAA40825.1| GTP-binding protein 1.0E-130
Protein Domains
Database ID Description E-Value
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.0E-126
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:188719_AT
ttcggccggctactatctgagccaactcgacagaatttgtgctcctaattacattcccac
acaggatgacattctccgcacaagaatcaagacaacaggaatcgtggagacgcagtttgt
ctacaaagatcgcctatttttggttttcgacgttggcggacagcgatccgaacgaaaaaa
gtggattcattgcttcgaagacgtgacggcactcattttctgcgttgcactgtcagaata
tgatatggttcttgtcgaagattgtcagacgaatcgaatgcgagaatcgctaaaactgtt
cgactcgatttgcaacaataaatggtttgtcgagacgtcgattattctatttctcaacaa
aaaagacttattcgaagagaaaatcgttcgatctccactcacacactgctttccggaata
tacgggcgccaataattacgaagaagcttctgcgtacattcaacaacagtttgaggatat
gaataagaggactactggagagaaaaatcaagaaatctacacccaattcacatgtgccac
cgacactaacaacattcgattcgttttcgatgccgtcaccgacattatcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCGGCCGGCTACTATCTGAGCCAA 297 695 462 Antisense
TGTGCTCCTAATTACATTCCCACAC 386 557 499 Antisense
CACACAGGATGACATTCTCCGCACA 506 195 519 Antisense
TTGGTTTTCGACGTTGGCGGACAGC 680 699 601 Antisense
GAAGACGTGACGGCACTCATTTTCT 571 339 658 Antisense
GACGTCGATTATTCTATTTCTCAAC 704 377 795 Antisense
AGAAAATCGTTCGATCTCCACTCAC 435 73 839 Antisense
TCACACACTGCTTTCCGGAATATAC 189 629 860 Antisense
GAAGCTTCTGCGTACATTCAACAAC 23 343 904 Antisense
CTACACCCAATTCACATGTGCCACC 404 227 978 Antisense
TCGATGCCGTCACCGACATTATCAT 324 603 1028 Antisense
Sequence Source Affymetrix Proprietary Database