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[188715_at] Ras family

Description Y45F3A.2 /REP_DB=WormBase Gene ID /WP=CE19165 /TR=Q9XWZ3 /GB=CAA21489.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Ras family
Cluster 6grp_high1_10_10 > 08      6grp_total > 09      4grp_total > 62      6grp_cgc > 0      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 91
1 2 3 4 range
A 61.2
(1.2)
P 		75.2
(3)
A 		28.6
(-3)
A 		57.5
(0.7)
A 		47
B 78.2
(1.6)
A 		73.2
(1.3)
A 		99.2
(3)
A 		8.3
(-3)
A 		91
C 91.6
(2.8)
A 		30
(-3)
A 		94.2
(3)
P 		79.6
(1.6)
M 		64
avr 77
(3) 		59.5
(-0.7) 		74
(2.4) 		48.5
(-3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 117
1 2 3 4 5 6 range
A 49.9
(-0.7)
A 		11.1
(-3)
A 		84.4
(1.3)
P 		94.9
(2)
P 		112.3
(3)
P 		40.2
(-1.3)
A 		101
B 117.5
(3)
P 		82.8
(-0.8)
P 		99
(1)
P 		73.9
(-1.8)
P 		86.6
(-0.4)
P 		63.2
(-3)
P 		54
C 128.5
(3)
P 		73.7
(-1.2)
M 		50.5
(-3)
A 		125.5
(2.8)
P 		76.7
(-1)
A 		74.1
(-1.2)
M 		78
avr 98.6
(3) 		55.9
(-3) 		78
(0.1) 		98.1
(2.9) 		91.9
(2.1) 		59.2
(-2.5) 		43
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 22.3
(3)
P 		16.3
(0.6)
A 		7.5
(-3)
A 		8.4
(-2.6)
A 		15
B 24.9
(3)
A 		18.1
(1.2)
A 		23
(2.5)
A 		1.8
(-3)
A 		23
C 30.3
(2.4)
A 		8.6
(-3)
A 		32.8
(3)
P 		13.4
(-1.8)
M 		24
avr 25.8
(3) 		14.3
(-0.9) 		21.1
(1.4) 		7.9
(-3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 9.2
(-2)
A 		4
(-3)
A 		18.4
(-0.1)
P 		24.1
(1)
P 		33.9
(3)
P 		9.8
(-1.8)
A 		30
B 40.9
(3)
P 		27.5
(-0.8)
P 		24
(-1.8)
P 		19.6
(-3)
P 		35.2
(1.4)
P 		23.6
(-1.9)
P 		21
C 47.6
(3)
P 		22.5
(-1.3)
M 		12.9
(-3)
A 		25.2
(-0.9)
P 		24.8
(-0.9)
A 		24.8
(-0.9)
M 		35
avr 32.6
(3) 		18
(-3) 		18.4
(-2.8) 		23
(-0.9) 		31.3
(2.5) 		19.4
(-2.4) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188715_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7917
Sequence Type Exemplar sequence
Representative Public ID Y45F3A.2 NCBI
Target Description Y45F3A.2 /REP_DB=WormBase Gene ID /WP=CE19165 /TR=Q9XWZ3 /GB=CAA21489.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Ras family
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066927(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066927 NCBI Caenorhabditis elegans RAB family member (rab-30) (rab-30) mRNA, complete cds. 11/11 None
GENEFINDER00000002987 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10562918:10565158:1 11/11 None
Y45F3A.2 ENSEMBL cdna:known chromosome:CEL140:III:10562903:10565158:1 gene:Y45F3A.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000002979 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10562913-10565154(+) 100.0 100.0
Public Domain and Genome References
Gene Title Ras family
Gene Symbol rab-30
Ensembl Y45F3A.2
Entrez Gene 176476 Entrez gene
SwissProt Q9XWZ3 EMBL-EBI
WormBase CE19165 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.7872.1.S1_AT similar to RAB30, member RAS oncogene family cfa
DROSOPHILA_2:1632557_S_AT dm
DROSGENOME1:145938_AT dm
CHICKEN:GGAAFFX.25213.1.S1_AT similar to RAB30 gga
CHICKEN:GGAAFFX.25213.2.S1_S_AT similar to RAB30 gga
HG-U95AV2:33599_AT RAB30, member RAS oncogene family hs
HG-U95AV2:807_AT RAB30, member RAS oncogene family hs
HC-G110:807_AT RAB30, member RAS oncogene family hs
HG-U133_PLUS_2:206530_AT RAB30, member RAS oncogene family hs
HG-U133A:206530_AT RAB30, member RAS oncogene family hs
HG-FOCUS:206530_AT RAB30, member RAS oncogene family hs
HG-U133A_2:206530_AT RAB30, member RAS oncogene family hs
HU35KSUBC:RC_AA455976_AT RAB30, member RAS oncogene family hs
HU35KSUBD:RC_AA458668_AT RAB30, member RAS oncogene family hs
HUGENEFL:U57092_AT RAB30, member RAS oncogene family hs
U133_X3P:G7657493_3P_AT RAB30, member RAS oncogene family hs
U133_X3P:HS.95898.0.S1_3P_AT RAB30, member RAS oncogene family hs
HG-U133_PLUS_2:227842_AT RAB30, member RAS oncogene family hs
HG-U133B:227842_AT RAB30, member RAS oncogene family hs
HG-U133_PLUS_2:228003_AT RAB30, member RAS oncogene family hs
HG-U133B:228003_AT RAB30, member RAS oncogene family hs
HG-U95B:51028_AT RAB30, member RAS oncogene family hs
HG-U95B:58691_AT RAB30, member RAS oncogene family hs
HG-U95B:58693_G_AT RAB30, member RAS oncogene family hs
HG-U95B:44704_AT RAB30, member RAS oncogene family hs
HG-U95C:59005_AT RAB30, member RAS oncogene family hs
HG-U95E:92117_AT RAB30, member RAS oncogene family hs
HG-U95E:92120_G_AT RAB30, member RAS oncogene family hs
HU35KSUBC:RC_H99054_AT RAB30, member RAS oncogene family hs
U133_X3P:HS.95898.0.S2_3P_AT RAB30, member RAS oncogene family hs
HU35KSUBD:RC_AA458945_AT RAB30, member RAS oncogene family hs
MU11KSUBB:MSA.4871.0_S_AT RAB30, member RAS oncogene family mm
MG-U74BV2:111057_AT RAB30, member RAS oncogene family mm
MOE430A:1426452_A_AT RAB30, member RAS oncogene family mm
MOUSE430A_2:1426452_A_AT RAB30, member RAS oncogene family mm
MOUSE430_2:1426452_A_AT RAB30, member RAS oncogene family mm
RAE230B:1379606_AT RAB30, member RAS oncogene family (predicted) rn
RAT230_2:1379606_AT RAB30, member RAS oncogene family (predicted) rn
RG-U34B:RC_AI044871_AT RAB30, member RAS oncogene family (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA21489 Hypothetical protein Y45F3A.2 [Caenorhabditis elegans] ref|NP_499328.1| RAB family member (rab-30) [Caenorhabditis elegans] 1.0E-121
blast CAE71446 Hypothetical protein CBG18357 [Caenorhabditis briggsae] 1.0E-118
blast EAL34458 GA21542-PA [Drosophila pseudoobscura] 1.0E-69
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 4.7E-69
Sequence
Target Sequence 
>C. ELEGANS:188715_AT
gtggtacttgtgggaaatgcgggcgttgggaaaacatgtctagtcagaaagttcacacag
ggtatatttcctcctggtcaaagtgcaacaatcggcgtcgattttatgataaaaactgtt
aaagtagggaacgataaaattaagcttcaaatttgggatacggcaggtcaggagaggttt
cgatcgattacgcagagttattacagaagcgcccacgcaatagttctagtgtacgatgta
tcgtgtcaaccatcatttgattgtcttccagaatggcttggtgaaatcgaaagttatgca
aatcggcgagttttgaaaattcttgttggaaacaaagtcgataagggagacgaacgggaa
gttcccgaacgaattggaagagatttttctgatgtcaaccaattcgactattttcttgaa
acttcggcacttgacgcgacaaatgtagatcaattgttcgaacaagtcgccacaaggtta
acaaatgatatgaaactcactgatgaacgggtacaccaattccgagcagacgcgacgaac
tcctcttccagcacgggcggtcccatcaaattaatcgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGGTACTTGTGGGAAATGCGGGCG 526 487 40 Antisense
GATACGGCAGGTCAGGAGAGGTTTC 247 405 196 Antisense
AGAGTTATTACAGAAGCGCCCACGC 66 67 233 Antisense
AGCGCCCACGCAATAGTTCTAGTGT 394 83 247 Antisense
GTCAACCATCATTTGATTGTCTTCC 161 465 284 Antisense
GTCAACCAATTCGACTATTTTCTTG 361 463 433 Antisense
TGAAACTTCGGCACTTGACGCGACA 229 571 456 Antisense
TGTTCGAACAAGTCGCCACAAGGTT 440 561 494 Antisense
ACTCACTGATGAACGGGTACACCAA 687 99 534 Antisense
TACACCAATTCCGAGCAGACGCGAC 504 643 551 Antisense
GGGCGGTCCCATCAAATTAATCGAT 112 493 594 Antisense
Sequence Source Affymetrix Proprietary Database