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[188707_s_at] peptidase

Description F18A12.5 /REP_DB=WormBase Gene ID /WP=CE23668 /TR=O16789 /GB=AAB66077.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=peptidase
Cluster 6grp_total > 77      4grp_total > 10      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 2.6
(-3)
A 		6
(-2.7)
A 		5.6
(-2.7)
A 		69.5
(3)
P 		67
B 4
(-3)
A 		7.4
(-1.8)
A 		4.6
(-2.8)
A 		20.9
(3)
A 		17
C 6.3
(0.2)
A 		4.4
(-1.4)
A 		2.5
(-3)
A 		9.7
(3)
A 		7
avr 4.3
(-3) 		5.9
(-2.7) 		4.2
(-3) 		33.4
(3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 12.8
(-1.1)
A 		2.7
(-2.9)
A 		2.3
(-3)
A 		2.5
(-3)
A 		11.8
(-1.3)
A 		36.3
(3)
A 		34
B 4.9
(-2.2)
A 		3.2
(-2.7)
A 		2.4
(-3)
A 		2.7
(-2.9)
A 		20.2
(3)
A 		15.3
(1.3)
A 		18
C 2.8
(-3)
A 		4.1
(-2.8)
A 		28
(1.1)
A 		14.1
(-1.1)
A 		2.6
(-3)
A 		39.7
(3)
A 		37
avr 6.8
(-2.2) 		3.3
(-3) 		10.9
(-1.3) 		6.4
(-2.3) 		11.5
(-1.2) 		30.4
(3) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 1
(-3)
A 		1.3
(-2.8)
A 		1.5
(-2.7)
A 		10.2
(3)
P 		9
B 1.3
(-2.6)
A 		1.8
(-1.7)
A 		1.1
(-3)
A 		4.4
(3)
A 		3
C 2.1
(3)
A 		1.3
(-1)
A 		0.9
(-3)
A 		1.6
(0.5)
A 		1
avr 1.5
(-2.6) 		1.5
(-2.6) 		1.2
(-3) 		5.4
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 2.4
(-1.6)
A 		1
(-2.6)
A 		0.5
(-3)
A 		0.6
(-2.9)
A 		3.6
(-0.8)
A 		8.8
(3)
A 		8
B 1.7
(-2.1)
A 		1.1
(-2.6)
A 		0.6
(-3)
A 		0.7
(-2.9)
A 		8.2
(3)
A 		5.7
(1)
A 		8
C 1.1
(-2.9)
A 		1.2
(-2.8)
A 		7.1
(0)
A 		2.8
(-2)
A 		0.8
(-3)
A 		13.3
(3)
A 		13
avr 1.7
(-2.6) 		1.1
(-3) 		2.7
(-1.8) 		1.4
(-2.8) 		4.2
(-0.7) 		9.3
(3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188707_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5167
Sequence Type Exemplar sequence
Representative Public ID F18A12.5 NCBI
Target Description F18A12.5 /REP_DB=WormBase Gene ID /WP=CE23668 /TR=O16789 /GB=AAB66077.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=peptidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062130(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062130 NCBI Caenorhabditis elegans F18A12.5 (peptidase) mRNA, complete cds. 11/11 None
SNAP00000014572 ENSEMBL cdna:SNAP chromosome:CEL140:II:3395839:3399005:-1 11/11 None
GENEFINDER00000014589 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3395853:3402049:-1 11/11 None
F18A12.5 ENSEMBL cdna:known chromosome:CEL140:II:3395816:3399298:-1 gene:F18A12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3395837-3399255(-) 100.0 100.0
Public Domain and Genome References
Gene Title peptidase
Ensembl F18A12.5
Entrez Gene 173683 Entrez gene
SwissProt O16789 EMBL-EBI
WormBase CE35493 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB66077 Hypothetical protein F18A12.5 [Caenorhabditis elegans] ref|NP_494531.2| F18A12.5 [Caenorhabditis elegans] 0.0
blast AAB66123 Hypothetical protein T06D4.4 [Caenorhabditis elegans] ref|NP_494530.1| T06D4.4 [Caenorhabditis elegans] 0.0
blast AAB66078 Hypothetical protein F18A12.6 [Caenorhabditis elegans] ref|NP_494532.1| F18A12.6 [Caenorhabditis elegans] 0.0
blast AAB66080 Hypothetical protein F18A12.1 [Caenorhabditis elegans] ref|NP_494537.1| F18A12.1 [Caenorhabditis elegans] 5.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR008753 EMBL-EBI Peptidase M13 5.5E-5
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 7.3E-33
Pfam IPR008753 EMBL-EBI Peptidase M13 5.5E-5
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 5.2E-62
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.3E-29
Pfam IPR008753 EMBL-EBI Peptidase M13 5.5E-5
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 7.3E-33
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494531.2 1 31-53
F18A12.5 1 31-53
Sequence
Target Sequence 
>C. ELEGANS:188707_S_AT
tttgctccgatcatcgatggtccatattttgatacctcctaccctaactacgtgaaccag
ttcctcatgggattcatcctaggccgagaactgggccgctcgctagattcagtgaggatt
cggaaagatgagaagggccaaactgagaattggttgaagccggagagcttgaaaagattc
gatgagcacctgaaatgttgggctgaaaagtatgataaatctgcggatccgcttaagtcg
gaaagtaaaggtatccgccaaatcgaagggagatatatccaaaacgagatgtttgccgac
ttcatcggacatgaagcgtcttggagagcctttatgaagttggatgtaaagaacgaagag
gtgctcgagacgtttaaggttattaagctgagaaggatgttcttcaatgtgcaggcattg
tacttctgccgtccgccgagtggctcgactgattggctatcctaccaggagcatcatcaa
cattactcgttccgtgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTGCTCCGATCATCGATGGTCCAT 350 669 1549 Antisense
GGTCCATATTTTGATACCTCCTACC 162 503 1567 Antisense
GAACCAGTTCCTCATGGGATTCATC 524 343 1602 Antisense
TCCTAGGCCGAGAACTGGGCCGCTC 490 547 1625 Antisense
GCCGCTCGCTAGATTCAGTGAGGAT 594 285 1643 Antisense
GATGTTTGCCGACTTCATCGGACAT 540 409 1836 Antisense
GAAGCGTCTTGGAGAGCCTTTATGA 383 343 1861 Antisense
TGTGCAGGCATTGTACTTCTGCCGT 706 555 1956 Antisense
CGAGTGGCTCGACTGATTGGCTATC 243 249 1985 Antisense
GATTGGCTATCCTACCAGGAGCATC 482 435 1999 Antisense
TCATCAACATTACTCGTTCCGTGTC 526 619 2022 Antisense
Sequence Source Affymetrix Proprietary Database