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[188704_s_at]

Description F18A12.8 /REP_DB=WormBase Gene ID /WP=CE23669 /TR=O16796 /GB=AAB66079.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=peptidase
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 17      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 60      6grp_total > 16      4grp_total > 22      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 863
1 2 3 4 range
A 987.3
(1.8)
P 		458.6
(-2)
P 		311.4
(-3)
P 		1155.4
(3)
P 		844
B 1063.1
(3)
P 		355.3
(-2.8)
P 		330
(-3)
P 		968.5
(2.2)
P 		733
C 1106.8
(3)
P 		433.8
(-2)
P 		292.7
(-3)
P 		882.9
(1.3)
P 		814
avr 1052.4
(3) 		415.9
(-2.2) 		311.4
(-3) 		1002.3
(2.6) 		741

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 560
1 2 3 4 5 6 range
A 666.3
(3)
P 		443.2
(-0.3)
P 		349.2
(-1.7)
P 		259.4
(-3)
P 		304
(-2.3)
P 		306.9
(-2.3)
P 		407
B 790.3
(3)
P 		595.9
(0.6)
P 		322.7
(-2.8)
P 		303.6
(-3)
P 		324
(-2.7)
P 		332.7
(-2.6)
P 		487
C 717.2
(3)
P 		486.4
(0.2)
P 		318.3
(-1.9)
P 		269.1
(-2.5)
P 		293.6
(-2.2)
P 		230.2
(-3)
P 		487
avr 724.6
(3) 		508.5
(0.1) 		330.1
(-2.3) 		277.4
(-3) 		307.2
(-2.6) 		289.9
(-2.8) 		447
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 290
1 2 3 4 range
A 359.5
(3)
P 		99.6
(-2.6)
P 		81.4
(-3)
P 		169.1
(-1.1)
P 		278
B 339
(3)
P 		87.7
(-2.7)
P 		76.5
(-3)
P 		206.4
(-0)
P 		263
C 366.4
(3)
P 		124.7
(-2.5)
P 		102
(-3)
P 		148.9
(-1.9)
P 		264
avr 355
(3) 		104
(-2.6) 		86.6
(-3) 		174.8
(-1) 		268

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 221
1 2 3 4 5 6 range
A 122.8
(0.7)
P 		157.6
(3)
P 		76
(-2.3)
P 		65.8
(-3)
P 		91.7
(-1.3)
P 		74.7
(-2.4)
P 		92
B 275
(3)
P 		198.3
(0.7)
P 		78.4
(-3)
P 		80.4
(-2.9)
P 		131.6
(-1.4)
P 		124.1
(-1.6)
P 		197
C 265.8
(3)
P 		148.4
(-0.3)
P 		81
(-2.2)
P 		54.1
(-3)
P 		95
(-1.8)
P 		77.1
(-2.3)
P 		212
avr 221.2
(3) 		168.1
(0.9) 		78.5
(-2.5) 		66.8
(-3) 		106.1
(-1.5) 		92
(-2) 		154
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188704_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4913
Sequence Type Exemplar sequence
Representative Public ID F18A12.8 NCBI
Target Description F18A12.8 /REP_DB=WormBase Gene ID /WP=CE23669 /TR=O16796 /GB=AAB66079.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=peptidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182128(11), NM_062137(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182128 NCBI Caenorhabditis elegans F18A12.8b (peptidase) mRNA, complete cds. 11/11 None
NM_062137 NCBI Caenorhabditis elegans F18A12.8a (peptidase) mRNA, complete cds. 11/11 None
F18A12.8b ENSEMBL cdna:known chromosome:CEL140:II:3420661:3425527:-1 gene:F18A12.8 11/11 A 
F18A12.8a ENSEMBL cdna:known chromosome:CEL140:II:3420661:3425527:-1 gene:F18A12.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3420995-3425526(-) 100.0 100.0
Public Domain and Genome References
Ensembl F18A12.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN65322 Hypothetical protein F18A12.8b [Caenorhabditis elegans] ref|NP_871928.1| F18A12.8b [Caenorhabditis elegans] 0.0
blast AAB66079 Hypothetical protein F18A12.8a [Caenorhabditis elegans] ref|NP_494538.1| F18A12.8a [Caenorhabditis elegans] 0.0
blast CAE70962 Hypothetical protein CBG17774 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008753 EMBL-EBI Peptidase M13 8.9E-82
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 2.2E-65
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 4.1E-87
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_871928.1 1 75-97
NP_494538.1 1 75-97
F18A12.8b 1 75-97
F18A12.8a 1 75-97
Sequence
Target Sequence 
>C. ELEGANS:188704_S_AT
gttctactcccccgaaaagaatgcaatcacattcccagcgggcattctgcaacctccgtt
cttctccggcacatttcccaaggcagtgaattacggagcaattggagcagtgattgggca
cgagattactcatggattcgatgatcaaggatcacagtatgataaggacggaaatttgca
caattggtggagtgagagctcgttgaattcgtttgacacaaggaggaggtgtattgtcga
gcagtacgggaactatacagtgcccaagactaactttagggtgaacggaaaactgaccca
aggagagaacattgccgacaacggtggagtcaaagaagcgttccaagcctatcagaaata
tgtgaccgaaaatggagaagagcctaggttacccggtctccagcagtacacgaatgagca
gatcttctttgtcagctatgcacatttctggtgtggcaagaagaaagaagccgcagcgat
gcaacaagtgctcaccgatgagcattctccagaggtgttccgagtaattggtgtcctgtc
gaatatgcaagcttttgctgacgtctacaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCTACTCCCCCGAAAAGAATGCA 681 443 1938 Antisense
CCGGCACATTTCCCAAGGCAGTGAA 349 265 2003 Antisense
GAGCTCGTTGAATTCGTTTGACACA 282 387 2133 Antisense
AAAGAAGCGTTCCAAGCCTATCAGA 78 123 2269 Antisense
GAGAAGAGCCTAGGTTACCCGGTCT 536 389 2312 Antisense
GGTCTCCAGCAGTACACGAATGAGC 436 503 2332 Antisense
CAGATCTTCTTTGTCAGCTATGCAC 677 205 2356 Antisense
TGAGCATTCTCCAGAGGTGTTCCGA 565 569 2436 Antisense
GTGTTCCGAGTAATTGGTGTCCTGT 303 485 2452 Antisense
GGTGTCCTGTCGAATATGCAAGCTT 413 499 2467 Antisense
GCAAGCTTTTGCTGACGTCTACAAG 328 321 2484 Antisense
Sequence Source Affymetrix Proprietary Database