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[188702_at] luciferase

Description C25G6.3 /REP_DB=WormBase Gene ID /WP=CE04084 /TR=Q18176 /GB=AAA82354.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=luciferase
Cluster 6grp_total > 32      4grp_total > 50      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 3.5
(-3)
A 		81.1
(3)
A 		43.3
(0.1)
A 		34.3
(-0.6)
A 		78
B 62.5
(3)
A 		61.6
(2.9)
A 		46.5
(0.8)
A 		19.6
(-3)
A 		43
C 40.1
(-3)
A 		62.2
(3)
A 		60.4
(2.5)
A 		46.6
(-1.2)
P 		22
avr 35.4
(-2.7) 		68.3
(3) 		50.1
(-0.1) 		33.5
(-3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 38.9
(1.7)
A 		39.1
(1.7)
A 		48.5
(3)
A 		26.1
(-0.1)
A 		34.8
(1.1)
A 		5.6
(-3)
A 		43
B 18.9
(-0.8)
A 		16.7
(-1.2)
A 		40.1
(3)
A 		14.7
(-1.6)
A 		37.6
(2.5)
M 		6.8
(-3)
A 		33
C 22.9
(0.2)
A 		37
(3)
A 		22.6
(0.2)
A 		27.4
(1.1)
A 		6.5
(-3)
A 		11.6
(-2)
A 		31
avr 26.9
(0.9) 		30.9
(1.7) 		37.1
(3) 		22.7
(0) 		26.3
(0.8) 		8
(-3) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 1.3
(-3)
A 		17.6
(3)
A 		11.3
(0.7)
A 		5
(-1.6)
A 		16
B 19.9
(3)
A 		15.2
(1.2)
A 		10.8
(-0.5)
A 		4.2
(-3)
A 		16
C 13.3
(-0.5)
A 		17.9
(1.6)
A 		21
(3)
A 		7.9
(-3)
P 		13
avr 11.5
(0.1) 		16.9
(3) 		14.4
(1.7) 		5.7
(-3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 7.2
(-0.2)
A 		13.9
(3)
A 		10.6
(1.4)
A 		6.6
(-0.5)
A 		10.5
(1.4)
A 		1.4
(-3)
A 		13
B 6.6
(-1.1)
A 		5.5
(-1.6)
A 		9.7
(0.4)
A 		3.9
(-2.3)
A 		15.3
(3)
M 		2.5
(-3)
A 		13
C 8.5
(1.2)
A 		11.3
(3)
A 		5.7
(-0.7)
A 		5.5
(-0.8)
A 		2.1
(-3)
A 		3.9
(-1.8)
A 		9
avr 7.4
(0.8) 		10.2
(3) 		8.7
(1.8) 		5.3
(-0.9) 		9.3
(2.3) 		2.6
(-3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188702_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18380
Sequence Type Exemplar sequence
Representative Public ID C25G6.3 NCBI
Target Description C25G6.3 /REP_DB=WormBase Gene ID /WP=CE04084 /TR=Q18176 /GB=AAA82354.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=luciferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075830(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075830 NCBI Caenorhabditis elegans C25G6.3 (luciferase) mRNA, complete cds. 11/11 None
GENEFINDER00000031332 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:1233702:1238886:-1 11/11 None
SNAP00000031322 ENSEMBL cdna:SNAP chromosome:CEL140:X:1235457:1238886:-1 11/11 None
C25G6.3 ENSEMBL cdna:known chromosome:CEL140:X:1235023:1238886:-1 gene:C25G6.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:1235022-1238886(-) 100.0 100.0
Public Domain and Genome References
Gene Title luciferase
Ensembl C25G6.3
Entrez Gene 182919 Entrez gene
SwissProt Q18176 EMBL-EBI
WormBase CE04084 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA82354 Hypothetical protein C25G6.3 [Caenorhabditis elegans] ref|NP_508231.1| C25G6.3 [Caenorhabditis elegans] 0.0
blast CAE63563 Hypothetical protein CBG08050 [Caenorhabditis briggsae] 0.0
blast CAB01175 Hypothetical protein F38H4.1 [Caenorhabditis elegans] ref|NP_502239.1| F38H4.1 [Caenorhabditis elegans] 9.0E-15
Protein Families
Method ID Description E-Value
ec AC2L_HUMAN (Q9NUB1) Acetyl-coenzyme A synthetase 2-like, mitochondrial precursor (EC 6.2.1.1) (Acetate--CoA ligase 2) (Acetyl-CoA synthetase 2) 2.0E-73
ec AC2L_HUMAN (Q9NUB1) Acetyl-coenzyme A synthetase 2-like, mitochondrial precursor (EC 6.2.1.1) (Acetate--CoA ligase 2) (Acetyl-CoA synthetase 2) 1.0E-74
ec AC2L_HUMAN (Q9NUB1) Acetyl-coenzyme A synthetase 2-like, mitochondrial precursor (EC 6.2.1.1) (Acetate--CoA ligase 2) (Acetyl-CoA synthetase 2) 3.0E-75
Protein Domains
Database ID Description E-Value
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 3.4E-15
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 5.5E-9
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 2.3E-15
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 6.2E-8
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 4.4E-9
Sequence
Target Sequence 
>C. ELEGANS:188702_AT
gtatacttgcggagtcgacgatggatgtctggagagcgtctttattagatcgaatcgctg
ggattatcgagcaatataaggttaccctactcttctcaaactcccaactgctgaaatgtt
ttatcaaatatgaagttcacaaaatctacaacttatcatcactcgaaattgtagccaacc
atggggctgtggtatcagtggccactgccaaaaaagtcaagaaaatcttagacgtcaccc
ttatccaagcatactctgccgcagagtttggtgttgccagttttggagattttgatgaaa
cattggataagcagctcgtcaactgtgggcactcaattgatgatgtcaaaatcaaagtaa
tagcccatatggaaactgacaaggaaatggcaaaggggaagtgggggcaagttattttga
gtgggcggcagttgctcaaagag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTATACTTGCGGAGTCGACGATGGA 650 453 992 Antisense
GATGGATGTCTGGAGAGCGTCTTTA 633 407 1011 Antisense
TATAAGGTTACCCTACTCTTCTCAA 376 659 1066 Antisense
TTCTCAAACTCCCAACTGCTGAAAT 182 689 1084 Antisense
AATCTACAACTTATCATCACTCGAA 601 169 1134 Antisense
GGCTGTGGTATCAGTGGCCACTGCC 309 541 1176 Antisense
GAAAATCTTAGACGTCACCCTTATC 288 351 1212 Antisense
ATCCAAGCATACTCTGCCGCAGAGT 675 39 1234 Antisense
GCCGCAGAGTTTGGTGTTGCCAGTT 499 285 1249 Antisense
TAAGCAGCTCGTCAACTGTGGGCAC 710 635 1299 Antisense
GAGTGGGCGGCAGTTGCTCAAAGAG 691 401 1410 Antisense
Sequence Source Affymetrix Proprietary Database