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[188698_s_at] esterase

Description T28C12.4A /REP_DB=WormBase Gene ID /WP=CE14343 /TR=O16695 /GB=AAB66159.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=esterase
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 77      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 99      6grp_total > 77      4grp_total > 02      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5033
1 2 3 4 range
A 286.7
(-3)
P 		390.1
(-2.9)
P 		383.2
(-2.9)
P 		5311.6
(3)
P 		5025
B 278.7
(-3)
P 		387.5
(-2.9)
P 		385.1
(-2.9)
P 		5094.7
(3)
P 		4816
C 307.9
(-3)
P 		394
(-2.9)
P 		435.9
(-2.8)
P 		4881.9
(3)
P 		4574
avr 291.1
(-3) 		390.5
(-2.9) 		401.4
(-2.9) 		5096.1
(3) 		4805

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3438
1 2 3 4 5 6 range
A 547.4
(-3)
P 		733.3
(-2.7)
P 		615
(-2.9)
P 		814.4
(-2.5)
P 		917.7
(-2.3)
P 		3903.9
(3)
P 		3357
B 503.9
(-3)
P 		757.9
(-2.6)
P 		616.6
(-2.8)
P 		775
(-2.5)
P 		1013.6
(-2.1)
P 		3901.8
(3)
P 		3398
C 466.2
(-3)
P 		693.6
(-2.6)
P 		806.3
(-2.4)
P 		737.6
(-2.5)
P 		951.8
(-2.1)
P 		3821.5
(3)
P 		3355
avr 505.8
(-3) 		728.3
(-2.6) 		679.3
(-2.7) 		775.7
(-2.5) 		961
(-2.2) 		3875.7
(3) 		3370
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1001
1 2 3 4 range
A 104.4
(-2.8)
P 		84.7
(-3)
P 		100.2
(-2.9)
P 		777.5
(3)
P 		693
B 88.9
(-3)
P 		95.7
(-3)
P 		89.2
(-3)
P 		1085.7
(3)
P 		997
C 101.9
(-3)
P 		113.3
(-2.9)
P 		151.9
(-2.6)
P 		823.2
(3)
P 		721
avr 98.4
(-3) 		97.9
(-3) 		113.8
(-2.9) 		895.5
(3) 		798

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1354
1 2 3 4 5 6 range
A 100.9
(-3)
P 		260.8
(-1.9)
P 		133.9
(-2.8)
P 		206.5
(-2.3)
P 		276.9
(-1.8)
P 		950.5
(3)
P 		850
B 175.3
(-2.9)
P 		252.3
(-2.5)
P 		149.7
(-3)
P 		205.3
(-2.7)
P 		411.8
(-1.8)
P 		1455
(3)
P 		1305
C 172.8
(-2.9)
P 		211.6
(-2.7)
P 		205.2
(-2.7)
P 		148.3
(-3)
P 		308
(-2.2)
P 		1279.3
(3)
P 		1131
avr 149.7
(-3) 		241.6
(-2.5) 		162.9
(-2.9) 		186.7
(-2.8) 		332.2
(-2) 		1228.3
(3) 		1079
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188698_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12249
Sequence Type Exemplar sequence
Representative Public ID T28C12.4A NCBI
Target Description T28C12.4A /REP_DB=WormBase Gene ID /WP=CE14343 /TR=O16695 /GB=AAB66159.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=esterase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072211(11), NM_072213(11), NM_072212(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072211 NCBI Caenorhabditis elegans T28C12.5 (esterase) mRNA, complete cds. 11/11 A 
NM_072213 NCBI Caenorhabditis elegans T28C12.4b (esterase) mRNA, complete cds. 11/11 A 
NM_072212 NCBI Caenorhabditis elegans T28C12.4a (esterase) mRNA, complete cds. 11/11 A 
SNAP00000017143 ENSEMBL cdna:SNAP chromosome:CEL140:V:6316770:6319061:1 11/11 None
SNAP00000017145 ENSEMBL cdna:SNAP chromosome:CEL140:V:6326242:6328787:1 11/11 None
GENEFINDER00000017154 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6325876:6328787:1 11/11 None
GENEFINDER00000017155 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6316770:6319061:1 11/11 None
T28C12.5 ENSEMBL cdna:known chromosome:CEL140:V:6316770:6319061:1 gene:T28C12.5 11/11 A 
T28C12.4b ENSEMBL cdna:known chromosome:CEL140:V:6319526:6328787:1 gene:T28C12.4 11/11 A 
T28C12.4a ENSEMBL cdna:known chromosome:CEL140:V:6325831:6328984:1 gene:T28C12.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6325875-6328787(+) 100.0 100.0
Public Domain and Genome References
Gene Title esterase
Ensembl T28C12.5
T28C12.4
Entrez Gene 189039 Entrez gene
SwissProt O16694 EMBL-EBI
WormBase CE14347 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB66162 Hypothetical protein T28C12.5 [Caenorhabditis elegans] ref|NP_504612.1| T28C12.5 [Caenorhabditis elegans] 0.0
blast AAB66160 Hypothetical protein T28C12.4b [Caenorhabditis elegans] ref|NP_504614.1| T28C12.4b [Caenorhabditis elegans] pir||F89068 protein T28C12.4b [imported] - Caenorhabditis elegans 0.0
blast AAB66159 Hypothetical protein T28C12.4a [Caenorhabditis elegans] ref|NP_504613.1| T28C12.4a [Caenorhabditis elegans] pir||T32053 hypothetical protein T28C12.4a - Caenorhabditis elegans 0.0
blast CAE71863 Hypothetical protein CBG18911 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.9E-38
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.7E-64
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 7.4E-29
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.0E-126
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.0E-126
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 2.1E-37
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.6E-106
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 8.6E-39
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 3.9E-79
Sequence
Target Sequence 
>C. ELEGANS:188698_S_AT
ggtttatggtgattcgccagcactgagagcagactcgaaagccagaaaaatgttctttgt
gcagcttatctccgacattttcgccaactatggaaactatcggtttatgagagactgcca
acagcgaggtgtcgaatgctatggctattcatttgaccatcaaagtaaacaaatgtgggg
atggctacagcacgtggttccatttaccggtggaactcatacctctgaactttcttacct
tttcgactgcaattacatgtccgcgccacttggaatgaacaaaaccgacaaagtggttag
tggaatgaccgccgattacttcacaaacttcgtcaagtttggaacaccaaacggtccgaa
ttcccaattaccaaaatgggaacggatttcaccagacgatgagcacatgaaattgatcag
tataaaaccagaaccaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTTTATGGTGATTCGCCAGCACTG 492 505 1389 Antisense
GATTCGCCAGCACTGAGAGCAGACT 205 433 1399 Antisense
GGTTTATGAGAGACTGCCAACAGCG 491 505 1490 Antisense
GTGTCGAATGCTATGGCTATTCATT 562 487 1517 Antisense
TGCTATGGCTATTCATTTGACCATC 156 579 1525 Antisense
ATGTGGGGATGGCTACAGCACGTGG 408 49 1561 Antisense
TTACCGGTGGAACTCATACCTCTGA 549 669 1592 Antisense
GTTAGTGGAATGACCGCCGATTACT 557 445 1684 Antisense
AAATGGGAACGGATTTCACCAGACG 531 119 1762 Antisense
GGATTTCACCAGACGATGAGCACAT 612 509 1772 Antisense
TGATCAGTATAAAACCAGAACCAGA 239 565 1802 Antisense
Sequence Source Affymetrix Proprietary Database