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[188674_at]

Description F32A5.2 /REP_DB=WormBase Gene ID /WP=CE01934 /CHR=2 /FEA=Sanger Annotation /DEF=Peroxidase (ST.LOUIS) TR:Q19950 protein_id:AAC46666.1
Cluster 6grp_highest_10_10 > 43      6grp_high1_10_10 > 33      6grp_highest_nonorm > 52      6grp_total > 21      4grp_total > 59      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 877
1 2 3 4 range
A 622.6
(1.9)
P 		754.2
(3)
P 		648.9
(2.1)
P 		37.2
(-3)
A 		717
B 494.5
(0.9)
P 		739.7
(3)
P 		684.9
(2.5)
P 		53.5
(-3)
A 		686
C 599.7
(1.1)
P 		767.9
(2.2)
P 		881.5
(3)
P 		4.1
(-3)
A 		877
avr 572.3
(1.5) 		753.9
(3) 		738.4
(2.9) 		31.6
(-3) 		722

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 887
1 2 3 4 5 6 range
A 522.6
(-1.3)
P 		1102.4
(3)
P 		724.5
(0.2)
P 		708.9
(0)
P 		550.1
(-1.1)
P 		302.4
(-3)
P 		800
B 548.4
(-1)
P 		1146.4
(3)
P 		624.4
(-0.5)
P 		566.3
(-0.9)
P 		571.5
(-0.9)
P 		259.7
(-3)
P 		887
C 616.2
(-0.3)
P 		1032.2
(3)
P 		1022.9
(2.9)
P 		608.5
(-0.3)
P 		741.4
(0.7)
P 		272.3
(-3)
P 		760
avr 562.4
(-0.9) 		1093.7
(3) 		790.6
(0.8) 		627.9
(-0.4) 		621
(-0.5) 		278.1
(-3) 		816
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 307
1 2 3 4 range
A 226.7
(3)
P 		163.7
(1.3)
P 		169.6
(1.5)
P 		5.4
(-3)
A 		221
B 157.7
(2.1)
P 		182.7
(3)
P 		158.7
(2.2)
P 		11.4
(-3)
A 		171
C 198.5
(0.9)
P 		220.8
(1.3)
P 		307.2
(3)
P 		0.7
(-3)
A 		307
avr 194.3
(2.5) 		189.1
(2.3) 		211.8
(3) 		5.8
(-3) 		206

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 319
1 2 3 4 5 6 range
A 96.3
(-2.6)
P 		392.1
(3)
P 		157.8
(-1.4)
P 		179.8
(-1)
P 		166
(-1.3)
P 		73.6
(-3)
P 		319
B 190.8
(-1)
P 		381.6
(3)
P 		151.6
(-1.8)
P 		150
(-1.9)
P 		232.2
(-0.1)
P 		96.9
(-3)
P 		285
C 228.4
(0.7)
P 		315
(3)
P 		260.4
(1.5)
P 		122.4
(-2.2)
P 		239.9
(1)
P 		91.1
(-3)
P 		224
avr 171.8
(-1.2) 		362.9
(3) 		189.9
(-0.8) 		150.7
(-1.6) 		212.7
(-0.3) 		87.2
(-3) 		276
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188674_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5303
Sequence Type Exemplar sequence
Representative Public ID F32A5.2 NCBI
Target Description F32A5.2 /REP_DB=WormBase Gene ID /WP=CE01934 /CHR=2 /FEA=Sanger Annotation /DEF=Peroxidase (ST.LOUIS) TR:Q19950 protein_id:AAC46666.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063110(11), NM_063111(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063110 NCBI Caenorhabditis elegans F32A5.2a (Peroxidase) mRNA, complete cds. 11/11 None
NM_063111 NCBI Caenorhabditis elegans F32A5.2b (Peroxidase) mRNA, complete cds. 11/11 None
SNAP00000015813 ENSEMBL cdna:SNAP chromosome:CEL140:II:7245849:7249909:1 11/11 None
GENEFINDER00000015826 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7245834:7249909:1 11/11 None
F32A5.2a ENSEMBL cdna:known chromosome:CEL140:II:7245846:7250105:1 gene:F32A5.2 11/11 A 
F32A5.2b ENSEMBL cdna:known chromosome:CEL140:II:7245849:7249909:1 gene:F32A5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7245844-7249786(+) 94.61 100.0
Public Domain and Genome References
Ensembl F32A5.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68356 Hypothetical protein F32A5.2a [Caenorhabditis elegans] ref|NP_495511.1| F32A5.2a [Caenorhabditis elegans] 0.0
blast T16232 hypothetical protein F32A5.2 - Caenorhabditis elegans 0.0
blast AAK68357 Hypothetical protein F32A5.2b [Caenorhabditis elegans] ref|NP_495512.1| F32A5.2b [Caenorhabditis elegans] 0.0
blast CAE67580 Hypothetical protein CBG13113 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 5.1E-12
Pfam IPR002007 EMBL-EBI Animal haem peroxidase 1.0E-126
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 5.1E-12
Pfam IPR002007 EMBL-EBI Animal haem peroxidase 1.0E-126
Pfam IPR002007 EMBL-EBI Animal haem peroxidase 1.0E-126
Pfam IPR002007 EMBL-EBI Animal haem peroxidase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:188674_AT
acgtcttgataattcattccgtaatatttcatttggtgcattgccattccaaaaaggaac
tcttcattctgatgttcttgtaaacgaaggaggagtcgatccattgatcagaggaatgtt
ttcccagaatgtgaaaagaccacaaagagtaacaactacagtaactgaaaacatgtttgg
atcaactgatctgtcaacaatcaatattcaaagaggaagagatcatggacatccagcata
tgttaaatacagagaattatgtggaatgggaactgcattcaactttgaacatttatcacg
agaaattttgaatactggaacaagaaacaagctgcaagaaatatatggatcagttgacaa
gattgatttatgggttggtgcactgcttgaggatccaatcattcgaggcttggttggacc
aactgttgcttgtataatcggcccacaattcaaaagaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGTCTTGATAATTCATTCCGTAAT 77 93 2343 Antisense
TTTCATTTGGTGCATTGCCATTCCA 143 671 2369 Antisense
AACTCTTCATTCTGATGTTCTTGTA 247 141 2400 Antisense
GGAGGAGTCGATCCATTGATCAGAG 630 515 2431 Antisense
GGATCAACTGATCTGTCAACAATCA 598 511 2521 Antisense
GATTTATGGGTTGGTGCACTGCTTG 311 429 2707 Antisense
GTGCACTGCTTGAGGATCCAATCAT 302 481 2720 Antisense
AATCATTCGAGGCTTGGTTGGACCA 98 169 2739 Antisense
GGTTGGACCAACTGTTGCTTGTATA 57 507 2754 Antisense
CTGTTGCTTGTATAATCGGCCCACA 368 239 2765 Antisense
TAATCGGCCCACAATTCAAAAGAAC 16 635 2777 Antisense
Sequence Source Affymetrix Proprietary Database