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[188656_at] peptidase

Description F18A12.4 /REP_DB=WormBase Gene ID /WP=CE09457 /TR=O16791 /GB=AAB66076.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=peptidase
Cluster 6grp_total > 67      4grp_total > 10      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 5
(-2.5)
A 		6
(-2.1)
A 		3.8
(-3)
A 		17.7
(3)
A 		14
B 2.3
(-3)
A 		3.6
(-2.7)
A 		3.4
(-2.7)
A 		27.2
(3)
A 		25
C 2.3
(-3)
A 		3.5
(-2.7)
A 		2.5
(-3)
A 		30.9
(3)
A 		29
avr 3.2
(-3) 		4.4
(-2.7) 		3.2
(-3) 		25.3
(3) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 4.4
(-2.9)
A 		4.4
(-2.9)
A 		5.9
(-2.7)
A 		3.8
(-3)
A 		8.1
(-2.3)
A 		43
(3)
A 		39
B 4.2
(-2.7)
A 		2
(-3)
A 		3.1
(-2.8)
A 		6.3
(-2.4)
A 		7.5
(-2.2)
A 		43.7
(3)
A 		42
C 3.9
(-3)
A 		3.6
(-3)
A 		4.9
(-2.8)
A 		7.1
(-2.5)
A 		6.6
(-2.6)
A 		45.8
(3)
P 		42
avr 4.2
(-2.9) 		3.3
(-3) 		4.6
(-2.8) 		5.7
(-2.6) 		7.4
(-2.4) 		44.2
(3) 		41
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 1.8
(0)
A 		1.3
(-1.9)
A 		1
(-3)
A 		2.6
(3)
A 		2
B 0.7
(-3)
A 		0.9
(-2.8)
A 		0.8
(-2.9)
A 		5.8
(3)
A 		5
C 0.8
(-3)
A 		1
(-2.7)
A 		0.9
(-2.9)
A 		5.2
(3)
A 		4
avr 1.1
(-2.7) 		1.1
(-2.7) 		0.9
(-3) 		4.5
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 0.8
(-3)
A 		1.6
(-2.5)
A 		1.3
(-2.7)
A 		1
(-2.9)
A 		2.4
(-2)
A 		10.5
(3)
A 		10
B 1.5
(-2.7)
A 		0.7
(-3)
A 		0.7
(-3)
A 		1.7
(-2.6)
A 		3
(-2.1)
A 		16.3
(3)
A 		16
C 1.4
(-2.9)
A 		1.1
(-3)
A 		1.2
(-3)
A 		1.4
(-2.9)
A 		2.1
(-2.6)
A 		15.3
(3)
P 		14
avr 1.2
(-3) 		1.1
(-3) 		1.1
(-3) 		1.4
(-2.9) 		2.5
(-2.3) 		14
(3) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188656_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5928
Sequence Type Exemplar sequence
Representative Public ID F18A12.4 NCBI
Target Description F18A12.4 /REP_DB=WormBase Gene ID /WP=CE09457 /TR=O16791 /GB=AAB66076.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=peptidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062132(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062132 NCBI Caenorhabditis elegans F18A12.4 (peptidase) mRNA, complete cds. 11/11 None
SNAP00000014575 ENSEMBL cdna:SNAP chromosome:CEL140:II:3403914:3406783:1 11/11 None
GENEFINDER00000014585 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3403914:3406783:1 11/11 None
F18A12.4 ENSEMBL cdna:known chromosome:CEL140:II:3403914:3406783:1 gene:F18A12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3403450-3406782(+) 100.0 100.0
Public Domain and Genome References
Gene Title peptidase
Ensembl F18A12.4
Entrez Gene 184631 Entrez gene
SwissProt O16791 EMBL-EBI
WormBase CE36827 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB66076 Hypothetical protein F18A12.4 [Caenorhabditis elegans] ref|NP_494533.2| F18A12.4 [Caenorhabditis elegans] 0.0
blast AAB66078 Hypothetical protein F18A12.6 [Caenorhabditis elegans] ref|NP_494532.1| F18A12.6 [Caenorhabditis elegans] 1.0E-175
blast AAB66078 Hypothetical protein F18A12.6 [Caenorhabditis elegans] ref|NP_494532.1| F18A12.6 [Caenorhabditis elegans] 1.0E-173
blast AAB66075 Hypothetical protein F18A12.3 [Caenorhabditis elegans] ref|NP_494534.2| F18A12.3 [Caenorhabditis elegans] 1.0E-153
blast AAB66078 Hypothetical protein F18A12.6 [Caenorhabditis elegans] ref|NP_494532.1| F18A12.6 [Caenorhabditis elegans] 1.0E-148
blast AAB66080 Hypothetical protein F18A12.1 [Caenorhabditis elegans] ref|NP_494537.1| F18A12.1 [Caenorhabditis elegans] 1.0E-134
Protein Domains
Database ID Description E-Value
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.6E-41
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.6E-41
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.6E-41
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494533.2 1 13-35
F18A12.4 1 13-35
SNAP00000014575 1 13-35
GENEFINDER00000014585 1 13-35
Sequence
Target Sequence 
>C. ELEGANS:188656_AT
aaaaacgctctaactgtctttgctccattcctcgacgatccattcttcgacatcacgtat
cccgactatgtgaacctctttttctccggcttccttattgcgcatgaaataggacattcc
gttgacgcaaatggacttggctatgatgaaaatggaaaacctatggatgggattagactc
gaacatgtaactgagtatgggaaacaaggtcaatgtctagtagatcaatatgataactat
gatgatccggtttacggcaaaaaaatgaatggaacacattcgataaacgagatcattgct
gattgggttgggcacgaagtaacctggagggcttataagaagatggatacttcaaaaatg
ctgtcgctcattggccttaaggatatcaatatggatcagctttatttcagagtccagtca
ctgttattctgtcgagaacgtgatacgataagcttggaagaaactctcaaagatgtatat
ccaacagacaatttccgtgtcaatggagcatatgccagagtttgcggaggcgttcaattg
tc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAACGCTCTAACTGTCTTTGCTC 90 131 1639 Antisense
GACATCACGTATCCCGACTATGTGA 534 369 1687 Antisense
CCGACTATGTGAACCTCTTTTTCTC 9 265 1700 Antisense
CGGCTTCCTTATTGCGCATGAAATA 138 243 1725 Antisense
GGACATTCCGTTGACGCAAATGGAC 202 525 1750 Antisense
GGGATTAGACTCGAACATGTAACTG 114 495 1807 Antisense
TGGGCACGAAGTAACCTGGAGGGCT 14 549 1947 Antisense
AAAAATGCTGTCGCTCATTGGCCTT 554 129 1992 Antisense
GTCCAGTCACTGTTATTCTGTCGAG 140 477 2050 Antisense
GTATATCCAACAGACAATTTCCGTG 351 451 2113 Antisense
GAGTTTGCGGAGGCGTTCAATTGTC 295 397 2156 Antisense
Sequence Source Affymetrix Proprietary Database