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[188654_at] histone h1

Description F22F1.1 /REP_DB=WormBase Gene ID /WP=CE04443 /TR=Q19743 /GB=AAA83282.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=histone h1
Cluster 6grp_highest_10_10 > 83      6grp_high1_10_10 > 38      4grp_highest_8_8 > 15      4grp_high_8_8 > 14      6grp_highest_nonorm > 02      6grp_total > 38      4grp_total > 10      6grp_highest_sub > Cu      6grp_cgc > 07      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8027
1 2 3 4 range
A 2200.9
(-3)
P 		2425.9
(-2.8)
P 		2723
(-2.6)
P 		9689.2
(3)
P 		7488
B 2081.3
(-3)
P 		2565.4
(-2.6)
P 		2630.6
(-2.6)
P 		10000.5
(3)
P 		7919
C 1974
(-3)
P 		2785
(-2.4)
P 		2389.6
(-2.7)
P 		9877.9
(3)
P 		7904
avr 2085.4
(-3) 		2592.1
(-2.6) 		2581.1
(-2.6) 		9855.9
(3) 		7771

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4551
1 2 3 4 5 6 range
A 5424.2
(3)
P 		2665.3
(-0.6)
P 		886.1
(-3)
P 		1093.8
(-2.7)
P 		1263
(-2.5)
P 		5260
(2.8)
P 		4538
B 5143.7
(3)
P 		2441.4
(-0.8)
P 		954
(-3)
P 		924.7
(-3)
P 		1155.9
(-2.7)
P 		3579.5
(0.8)
P 		4219
C 5410.9
(3)
P 		2250.8
(-1.2)
P 		1375.9
(-2.3)
P 		873.2
(-3)
P 		1710.4
(-1.9)
P 		3009.4
(-0.2)
P 		4538
avr 5326.3
(3) 		2452.5
(-1) 		1072
(-2.9) 		963.9
(-3) 		1376.4
(-2.4) 		3949.6
(1.1) 		4362
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1605
1 2 3 4 range
A 801.3
(-1.2)
P 		526.6
(-3)
P 		711.8
(-1.8)
P 		1418.3
(3)
P 		892
B 663.7
(-2.8)
P 		633.6
(-2.9)
P 		609.6
(-3)
P 		2131.1
(3)
P 		1522
C 653.5
(-3)
P 		800.7
(-2.1)
P 		832.9
(-1.9)
P 		1665.6
(3)
P 		1012
avr 706.2
(-2.7) 		653.6
(-3) 		718.1
(-2.6) 		1738.3
(3) 		1085

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1830
1 2 3 4 5 6 range
A 999.9
(1.5)
P 		947.9
(1.2)
P 		193
(-3)
P 		277.4
(-2.5)
P 		381.1
(-2)
P 		1280.6
(3)
P 		1088
B 1789.8
(3)
P 		812.6
(-0.8)
P 		231.7
(-3)
P 		244.9
(-2.9)
P 		469.6
(-2.1)
P 		1334.8
(1.2)
P 		1558
C 2005.6
(3)
P 		686.8
(-1.3)
P 		350.2
(-2.4)
P 		175.6
(-3)
P 		553.4
(-1.8)
P 		1007.4
(-0.3)
P 		1830
avr 1598.4
(3) 		815.8
(-0.4) 		258.3
(-2.9) 		232.6
(-3) 		468
(-2) 		1207.6
(1.3) 		1366
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188654_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17033
Sequence Type Exemplar sequence
Representative Public ID F22F1.1 NCBI
Target Description F22F1.1 /REP_DB=WormBase Gene ID /WP=CE04443 /TR=Q19743 /GB=AAA83282.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=histone h1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076974(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076974 NCBI Caenorhabditis elegans HIstone H1 Like family member (hil-3) (hil-3) mRNA, complete cds. 11/11 None
GENEFINDER00000034837 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8158280:8159817:1 11/11 None
SNAP00000034834 ENSEMBL cdna:SNAP chromosome:CEL140:X:8159136:8159817:1 11/11 None
F22F1.1 ENSEMBL cdna:known chromosome:CEL140:X:8159094:8160024:1 gene:F22F1.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8159134-8159816(+) 100.0 100.0
Public Domain and Genome References
Gene Title histone h1
Gene Symbol hil-3
Ensembl F22F1.1
Entrez Gene 181073 Entrez gene
SwissProt Q19743 EMBL-EBI
WormBase CE04443 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1631321_S_AT dm
DROSOPHILA_2:1629740_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6334 nucleosome assembly inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
786 nucleosome inferred from electronic annotation QuickGO AmiGO
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAM44399 Histone h1 like protein 2, isoform a [Caenorhabditis elegans] ref|NP_741417.3| HIstone H1 Like family member (hil-2) [Caenorhabditis elegans] 7.0E-37
blast AAA83282 Histone h1 like protein 3 [Caenorhabditis elegans] ref|NP_509375.1| HIstone H1 Like family member (hil-3) [Caenorhabditis elegans] sp|Q19743|H13_CAEEL Histone H1.3 (Histone H1-like protein 3) gb|AAB66471.1| histone H1.3 [Caenorhabditis elegans] 5.0E-36
blast AAA83282 Histone h1 like protein 3 [Caenorhabditis elegans] ref|NP_509375.1| HIstone H1 Like family member (hil-3) [Caenorhabditis elegans] sp|Q19743|H13_CAEEL Histone H1.3 (Histone H1-like protein 3) gb|AAB66471.1| histone H1.3 [Caenorhabditis elegans] 1.0E-35
blast AAM44399 Histone h1 like protein 2, isoform a [Caenorhabditis elegans] ref|NP_741417.3| HIstone H1 Like family member (hil-2) [Caenorhabditis elegans] 3.0E-25
blast P15796 Histone H1.2 (Histone H1-like protein 2) gb|AAB70666.1| histone H1.2 [Caenorhabditis elegans] gb|AAB70559.1| histone H1.2 [Caenorhabditis elegans] 3.0E-25
Protein Domains
Database ID Description E-Value
scop a.4.5.Histone H1/H5 All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Histone H1/H5 9.0000003502848E-15
scop a.4.5.Histone H1/H5 All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Histone H1/H5 7.00000021597487E-14
Pfam IPR005818 EMBL-EBI Histone H1/H5 1.8E-32
Pfam IPR005818 EMBL-EBI Histone H1/H5 1.8E-32
Sequence
Target Sequence 
>C. ELEGANS:188654_AT
gcagttcaggctccagccaagactgtcaagtctccaaaggccgccaagaccaccaaggtt
ccaaaagccaagaagccagttgcccatccaccatacattaacatggtcactgccgccatc
aatggtcttaaggaacgcaaaggatcttccaagatcgccatcctcaagtacatcaccaag
aactacaatgttggagatcaaatcatcaagattaatgctcgtctccgtgacactctcaac
aaaggagtcgtcagcaaggcactcgttcaatccgtcggaactggagccagtggacgcttc
cgtgttactgagaagaaggccgccgccgcaaagaagccagtagccaagaaggccgccacc
ggagagaagaaagcaaagaagccagttgcccagaaggccgccaccggagaaaagaaggcc
aagaagaccactgccacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAGTTCAGGCTCCAGCCAAGACTG 33 313 40 Antisense
AGACTGTCAAGTCTCCAAAGGCCGC 635 67 59 Antisense
GACCACCAAGGTTCCAAAAGCCAAG 499 383 87 Antisense
TGCCCATCCACCATACATTAACATG 565 583 120 Antisense
TCACTGCCGCCATCAATGGTCTTAA 189 627 146 Antisense
CCATCCTCAAGTACATCACCAAGAA 9 273 197 Antisense
TCAAGATTAATGCTCGTCTCCGTGA 417 627 245 Antisense
TCTCCGTGACACTCTCAACAAAGGA 391 611 261 Antisense
TCGTCAGCAAGGCACTCGTTCAATC 16 605 287 Antisense
TGGACGCTTCCGTGTTACTGAGAAG 304 549 330 Antisense
GAAGGCCAAGAAGACCACTGCCACC 380 333 453 Antisense
Sequence Source Affymetrix Proprietary Database