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[188649_at]

Description F31E8.6 /REP_DB=WormBase Gene ID /WP=CE01933 /GB=AAA98024.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=DNA topoisomerase II
Cluster 6grp_total > 43      4grp_total > 20      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 173
1 2 3 4 range
A 86.1
(-0.6)
A 		44.2
(-2)
A 		15.7
(-3)
A 		188.5
(3)
A 		173
B 62.1
(-3)
A 		82.4
(-0.4)
A 		91.8
(0.8)
A 		108.6
(3)
A 		47
C 78.8
(0.1)
A 		119
(3)
A 		34.4
(-3)
A 		94.6
(1.3)
A 		85
avr 75.7
(-1) 		81.9
(-0.5) 		47.3
(-3) 		130.6
(3) 		83

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 85.4
(1.5)
A 		89.1
(1.7)
A 		107.6
(3)
A 		19.1
(-3)
A 		21.8
(-2.8)
A 		32.1
(-2.1)
A 		89
B 61.1
(0.8)
A 		12.2
(-3)
A 		71.9
(1.7)
A 		89.2
(3)
A 		85.9
(2.7)
A 		34.2
(-1.3)
A 		77
C 84
(2.2)
A 		16.5
(-2.9)
A 		40.3
(-1.1)
A 		14.6
(-3)
A 		94
(3)
A 		50.8
(-0.3)
A 		79
avr 76.8
(3) 		39.3
(-3) 		73.3
(2.4) 		41
(-2.7) 		67.2
(1.5) 		39
(-3) 		38
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 31.3
(3)
A 		9.6
(-1.8)
A 		4.1
(-3)
A 		27.6
(2.2)
A 		27
B 19.8
(-3)
A 		20.3
(-2.1)
A 		21.3
(-0.3)
A 		23.1
(3)
A 		3
C 26.1
(0.8)
A 		34.2
(3)
A 		12
(-3)
A 		15.9
(-1.9)
A 		22
avr 25.7
(3) 		21.4
(1) 		12.5
(-3) 		22.2
(1.4) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 15.7
(-0.6)
A 		31.7
(3)
A 		23.4
(1.1)
A 		4.8
(-3)
A 		6.6
(-2.6)
A 		7.8
(-2.3)
A 		27
B 21.3
(0.4)
A 		4.1
(-3)
A 		17.5
(-0.4)
A 		23.6
(0.8)
A 		34.9
(3)
A 		12.8
(-1.3)
A 		31
C 31.1
(3)
A 		5
(-2.6)
A 		10.3
(-1.4)
A 		2.9
(-3)
A 		30.4
(2.9)
A 		17
(-0)
A 		28
avr 22.7
(2.4) 		13.6
(-1.6) 		17.1
(-0) 		10.4
(-3) 		24
(3) 		12.5
(-2.1) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188649_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5275
Sequence Type Exemplar sequence
Representative Public ID F31E8.6 NCBI
Target Description F31E8.6 /REP_DB=WormBase Gene ID /WP=CE01933 /GB=AAA98024.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=DNA topoisomerase II
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062995(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062995 NCBI Caenorhabditis elegans F31E8.6 (F31E8.6) mRNA, complete cds. 11/11 None
F31E8.6 ENSEMBL cdna:known chromosome:CEL140:II:6817680:6820418:-1 gene:F31E8.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000019080 8/11 Cross Hyb Matching Probes None
SNAP00000019081 2/11 Cross Hyb Matching Probes None
GENEFINDER00000019087 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6817674-6820413(-) 100.0 100.0
Public Domain and Genome References
Ensembl F31E8.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_495396 F31E8.6 [Caenorhabditis elegans] pir||T16227 hypothetical protein F31E8.6 - Caenorhabditis elegans 1.0E-152
blast CAA94177 Hypothetical protein K12D12.1 [Caenorhabditis elegans] emb|CAA88867.1| Hypothetical protein K12D12.1 [Caenorhabditis elegans] ref|NP_496536.1| K12D12.1 [Caenorhabditis elegans] sp|Q23670|TOP2_CAEEL Probable DNA topoisomerase 2 (DNA topoisomerase II) 3.0E-82
blast CAE59389 Hypothetical protein CBG02746 [Caenorhabditis briggsae] 9.0E-73
Protein Domains
Database ID Description E-Value
Pfam IPR001241 EMBL-EBI DNA topoisomerase II 1.1E-12
Sequence
Target Sequence 
>C. ELEGANS:188649_AT
gttcaccatggagacgtcttttcgtgactacaaaagtgcattcaagcaaacatggatcaa
caacatgacacgtgatgaggagcccaaaattgttgagagcacggagaaagacttcacgaa
gatcacgttctcgccggatctgtcaaagttcaagatgaaggagctggacgatctgacatc
tgatggctcgtcgtgcctatcgttggcaggtggcgttggcctttcggagaaaggttccca
gcaagtttccttcgtgaatagtattgcgaccacaaaaggaggacgtcatgtggattacgt
cgccgatcagatggtcacaaaattcatcgactccatcaagaagaaacttaccaagacgtc
gacgaatatcaagccgttccagaagttttccaaggcggcatcaagggttggaatcaccga
cgctgttatgacttcggttcgcttcaaacgaatggacgacctcaacaaaatgtgctccaa
gccggagacttccatcatgaaggacatctcaaggctggtggacgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCACCATGGAGACGTCTTTTCGT 131 445 255 Antisense
TGACATCTGATGGCTCGTCGTGCCT 369 571 428 Antisense
GTCGTGCCTATCGTTGGCAGGTGGC 110 473 444 Antisense
TTCCCAGCAAGTTTCCTTCGTGAAT 709 695 489 Antisense
TTACGTCGCCGATCAGATGGTCACA 679 679 549 Antisense
GTCGACGAATATCAAGCCGTTCCAG 585 473 612 Antisense
GAAGTTTTCCAAGGCGGCATCAAGG 215 337 636 Antisense
GAATCACCGACGCTGTTATGACTTC 620 327 665 Antisense
GTTATGACTTCGGTTCGCTTCAAAC 685 445 679 Antisense
TCCAAGCCGGAGACTTCCATCATGA 423 587 730 Antisense
GGACATCTCAAGGCTGGTGGACGCA 97 525 756 Antisense
Sequence Source Affymetrix Proprietary Database